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Difference between revisions of "O2k-Virtual Support self-study material"

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== General information ==  
== General information ==  
:::: The materials for self-study, with the names and links to materials, are recommended to be studied before the respective training sessions of the [[Virtual O2k-Workshops|Virtual O2k-Workshop]].
:::: To benefit optimally from the tutored training sessions of the Virtual O2k-Workshop, please make yourself familiar with '''Materials for self-study''' in advance. These materials are either Open Access (OA) or will be sent upon registration (R).


== O2k-Basic ==
== O2k-Basic ==
=== Part 1.1: OroboPOS service and O2k instrumental ===
=== Part 1.1: OroboPOS service and O2k instrumental ===
:::* [[O2k-FluoRespirometer|O2k-Manual: O2k-FluoRespirometer]]
:::* [[O2k-FluoRespirometer|O2k-Manual: O2k-FluoRespirometer]] (OA)
:::* [[MiPNet19.18B POS-service|O2k-Manual: POS Service]]
:::* [[MiPNet19.18B POS-service|O2k-Manual: POS Service]] (OA)
:::* [https://www.youtube.com/watch?v=wMDcSypG3O4&feature=youtu.be O2k-Videosupport: OroboPOS]
:::* [https://www.youtube.com/watch?v=wMDcSypG3O4&feature=youtu.be O2k-Videosupport: OroboPOS] (OA)
:::* [https://www.youtube.com/watch?v=HC6MLXO08k8&feature=youtu.be O2k-Videosupport: Cathode cleaning]
:::* [https://www.youtube.com/watch?v=HC6MLXO08k8&feature=youtu.be O2k-Videosupport: Cathode cleaning] (OA)
:::* [https://www.youtube.com/watch?v=sDoU69-SLq0&feature=youtu.be O2k-Videosupport: Anode cleaning]
:::* [https://www.youtube.com/watch?v=sDoU69-SLq0&feature=youtu.be O2k-Videosupport: Anode cleaning] (OA)
:::* [https://www.youtube.com/watch?v=iGafaPa4OyA&feature=youtu.be O2k-Videosupport: Membrane mounting]
:::* [https://www.youtube.com/watch?v=iGafaPa4OyA&feature=youtu.be O2k-Videosupport: Membrane mounting] (OA)
:::* [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 O2k-Videosupport: Start DatLab 7]
:::* [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 O2k-Videosupport: Start DatLab 7] (OA)
:::* [https://www.youtube.com/watch?v=pGzb0KfYwTQ&feature=youtu.be O2k-Videosupport: Insert OroboPOS]
:::* [https://www.youtube.com/watch?v=pGzb0KfYwTQ&feature=youtu.be O2k-Videosupport: Insert OroboPOS] (OA)
:::* [https://www.youtube.com/watch?v=1MSsI1ePMu8&feature=youtu.be O2k-Videosupport: Insert O2k Chamber]
:::* [https://www.youtube.com/watch?v=1MSsI1ePMu8&feature=youtu.be O2k-Videosupport: Insert O2k Chamber] (OA)
:::* [https://www.youtube.com/watch?v=DuaEJc7FBjk&feature=youtu.be O2k-Videosupport: O2k Chamber volume calibration]
:::* [https://www.youtube.com/watch?v=DuaEJc7FBjk&feature=youtu.be O2k-Videosupport: O2k Chamber volume calibration] (OA)


=== Part 1.2: DatLab ===
=== Part 1.2: DatLab ===
:::* [[MitoPedia: DatLab]]
:::* [[MitoPedia: DatLab]] (OA)
:::* [[Run DL-Protocol/Set O2 limit|DL-Protocols]]
:::* [[Run DL-Protocol/Set O2 limit|DL-Protocols]] (OA)


=== Part 1.3: O<sub>2</sub>calibration and instrumental background  ===
=== Part 1.3: O<sub>2</sub> calibration and instrumental background  ===
:::* [[Gnaiger 2008 POS]]
:::* [[Gnaiger 2008 POS]] (OA)
:::* [[MiPNet19.03 O2k-cleaning and ISS|SOP: O2k-cleaning and ISS]]
:::* [[MiPNet19.03 O2k-cleaning and ISS|SOP: O2k-cleaning and ISS]] (OA)
:::* [[MiPNet06.03 POS-calibration-SOP|SOP: O<sub>2</sub>-calibration]]
:::* [[MiPNet06.03 POS-calibration-SOP|SOP: O<sub>2</sub>-calibration]] (OA)
:::* O2k-Videosupport: QC1: POS calibration*
:::* O2k-Videosupport: QC1: POS calibration (R)
:::* [[MiPNet14.06 Instrumental O2 background|SOP: O<sub>2</sub> background]]
:::* [[MiPNet14.06 Instrumental O2 background|SOP: O<sub>2</sub> background]] (OA)


=== Part 2.1: Biological samples and experimental design ===
=== Part 2.1: Biological samples and experimental design ===
'''Pathway control and coupling control of mitochondrial respiration:'''
'''Pathway control and coupling control of mitochondrial respiration:'''
:::* [[Gnaiger 2020 MitoPathways|Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis]]
:::* [[Gnaiger 2020 MitoPathways|Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis]] (OA)
:::* [[BEC 2020.1 doi10.26124bec2020-0001.v1|BEC 2020.1 Mitochondrial Physiology]]
:::* [[BEC 2020.1 doi10.26124bec2020-0001.v1|BEC 2020.1 Mitochondrial Physiology]] (OA)


'''Sample types,mitochondrial preparations and sample addition:'''
'''Sample types,mitochondrial preparations and sample addition:'''
:::* [[Sample]]
:::* [[Sample]] (OA)
:::* [[Mitochondrial preparations]]
:::* [[Mitochondrial preparations]] (OA)
:::* [[MiPNet17.03 Shredder vs Fibres]]
:::* [[MiPNet17.03 Shredder vs Fibres]] (OA)
:::* [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be O2k-Videosupport: Shredder tissue homogenate]
:::* [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be O2k-Videosupport: Shredder tissue homogenate] (OA)
:::* [https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be O2k-Videosupport: Fiber permeabilization]
:::* [https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be O2k-Videosupport: Fiber permeabilization] (OA)
:::* [[Cell count and normalization in HRR]]
:::* [[Cell count and normalization in HRR]] (OA)
:::* O2k-Videosupport: Addition of biological sample to the respirometer chamber*
:::* O2k-Videosupport: Addition of biological sample to the respirometer chamber (R)


'''SUIT protocols, SUITbrowser, DL-Protocols:'''
'''SUIT protocols, SUITbrowser, DL-Protocols:'''
:::* [[MitoPedia: SUIT]]
:::* [[MitoPedia: SUIT]] (OA)
:::* [https://suitbrowser.oroboros.at/ Oroboros SUITbrowser]
:::* [https://suitbrowser.oroboros.at/ Oroboros SUITbrowser] (OA)
:::* [https://www.youtube.com/watch?v=8T33sp9KkJk O2k-Videosupport: How to find a DL-Protocol (DLP)]
:::* [https://www.youtube.com/watch?v=8T33sp9KkJk O2k-Videosupport: How to find a DL-Protocol (DLP)] (OA)
:::* [https://www.youtube.com/watch?v=Vd66dHx-MyI O2k-Videosupport: Export DL-Protocol User (*.DLPU)]
:::* [https://www.youtube.com/watch?v=Vd66dHx-MyI O2k-Videosupport: Export DL-Protocol User (*.DLPU)] (OA)


=== Part 2.2: Biological experiment and data analysis ===  
=== Part 2.2: Biological experiment and data analysis ===  
:::* [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4|Oxygen_flux_analysis]]
:::* [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4|Oxygen_flux_analysis]] (OA)
:::* [https://www.youtube.com/watch?v=HRAGvmaK01I O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4]
:::* [https://www.youtube.com/watch?v=HRAGvmaK01I O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4] (OA)




== O2k-Advanced ==
== O2k-Advanced ==
''' O2k-Applications: Simultaneous  determination  of  O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> fluxes'''  
''' O2k-Applications: Simultaneous  determination  of  O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> fluxes'''  
:::* [[Amplex UltraRed]]
:::* [[Amplex UltraRed]] (OA)
:::* O2k-Videosupport: Smart Fluo-Sensor assembly*
:::* O2k-Videosupport: Smart Fluo-Sensor assembly (R)
:::* O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor*
:::* O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor (R)
:::* [[Amplex_UltraRed#Calibration_with_H2O2|Calibration with H<sub>2</sub>O<sub>2</sub>]]
:::* [[Amplex_UltraRed#Calibration_with_H2O2|Calibration with H<sub>2</sub>O<sub>2</sub>]] (OA)
:::* [[MiPNet24.10 H2O2 flux analysis|<sub>2</sub>O<sub>2</sub> flux analysis]]
:::* [[MiPNet24.10 H2O2 flux analysis|H<sub>2</sub>O<sub>2</sub> flux analysis]] (OA)
:::* O2k-Videosupport: H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4*
:::* O2k-Videosupport: H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4 (R)




== NextGen-O2k KOL Training ==
== NextGen-O2k KOL Training ==
:::* O2k-Videosupport: SUIT-030_PB_ce_D069 demo experiment *
:::* PB-Module Manual (R)
:::* O2k-Videosupport: SUIT-030_PB_ce_D070 demo experiment*
:::* O2k-Videosupport: SUIT-030_PB_ce_D069 demo experiment (R)


* ''exclusive materials and links will be sent upon registration ''
:::* DL-Protocol: SUIT-030_PB_ce_D069 (R)
:::* O2k-Videosupport: SUIT-030_PB_ce_D070 demo experiment (R)
 
:::* DL-Protocol: SUIT-030_PB_ce_D070 (R)

Revision as of 13:36, 21 October 2020


                  


O2k-Open Support

O2k-Virtual Support self-study material



Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



COST Action MitoEAGLE

General information

To benefit optimally from the tutored training sessions of the Virtual O2k-Workshop, please make yourself familiar with Materials for self-study in advance. These materials are either Open Access (OA) or will be sent upon registration (R).

O2k-Basic

Part 1.1: OroboPOS service and O2k instrumental

Part 1.2: DatLab

Part 1.3: O2 calibration and instrumental background

Part 2.1: Biological samples and experimental design

Pathway control and coupling control of mitochondrial respiration:

Sample types,mitochondrial preparations and sample addition:

SUIT protocols, SUITbrowser, DL-Protocols:

Part 2.2: Biological experiment and data analysis


O2k-Advanced

O2k-Applications: Simultaneous determination of O2 and H2O2 fluxes


NextGen-O2k KOL Training

  • PB-Module Manual (R)
  • O2k-Videosupport: SUIT-030_PB_ce_D069 demo experiment (R)
  • DL-Protocol: SUIT-030_PB_ce_D069 (R)
  • O2k-Videosupport: SUIT-030_PB_ce_D070 demo experiment (R)
  • DL-Protocol: SUIT-030_PB_ce_D070 (R)