Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Warburg effect"

From Bioblast
Line 7: Line 7:
}}
}}
{{MitoPedia topics}}
{{MitoPedia topics}}
== MultiSensor-O2k: Oxygen and pH - Warburg versus Crabtree Effect ==
== Oxygen and pH - Warburg versus Crabtree Effect ==


'''Q:'''  For quantification of aerobic glycolysis in intact cells, the  measurement of proton production can be used as an indirect but  continuous record of lactate production and corresponding acidification  of the medium, while simultaneously monitoring oxygen concentration and  oxygen consumption ([[O2k-MultiSensor|MultiSensor-O2k]]). Is this related to the Warburg or Crabtree effect?  
:::: '''Q:'''  For quantification of aerobic glycolysis in intact cells, the  measurement of proton production can be used as an indirect but  continuous record of lactate production and corresponding acidification  of the medium, while simultaneously monitoring oxygen concentration and  oxygen consumption ([[O2k-MultiSensor|MultiSensor-O2k]]). Is this related to the Warburg or Crabtree effect?  


'''A:'''  Under various metabolic conditions, lactic acid production is the  dominant mechanism causing acidification, hence the pH measurement is a  good indirect indicator of aerobic glycolysis. An early paper  summarizing the literature in this field states:  
:::: '''A:'''  Under various metabolic conditions, lactic acid production is the  dominant mechanism causing acidification, hence the pH measurement is a  good indirect indicator of aerobic glycolysis. An early paper  summarizing the literature in this field states:  


"At  high fructose concen­trations, respiration is inhibited while glycolytic  end products accumulate, a phenomenon known as the Crabtree effect. It  is commonly believed that this effect is restric­ted to microbial and  tumour cells with uniquely high glycolytic capaci­ties (Sussman et al,  1980). How­ever, inhibition of respiration and increase of lactate  production are observed under aerobic condi­tions in beating rat heart  cell cultures (Frelin et al, 1974) and in isolated rat lung cells  (Ayuso-Parrilla et al, 1978). Thus, the same general mechanisms  respon­sible for the integra­tion of respiration and glycolysis in  tumour cells (Sussman et al, 1980) appear to be operating to some extent  in several isolated mammalian cells." [[Gnaiger 1990 Biochim Biophys  Acta]]
:::: "At  high fructose concen­trations, respiration is inhibited while glycolytic  end products accumulate, a phenomenon known as the Crabtree effect. It  is commonly believed that this effect is restric­ted to microbial and  tumour cells with uniquely high glycolytic capaci­ties (Sussman et al,  1980). How­ever, inhibition of respiration and increase of lactate  production are observed under aerobic condi­tions in beating rat heart  cell cultures (Frelin et al, 1974) and in isolated rat lung cells  (Ayuso-Parrilla et al, 1978). Thus, the same general mechanisms  respon­sible for the integra­tion of respiration and glycolysis in  tumour cells (Sussman et al, 1980) appear to be operating to some extent  in several isolated mammalian cells." [[Gnaiger 1990 Biochim Biophys  Acta]]




== From the literature ==
== From the literature ==
* Warburg O (1956) On the origin of cancer cells. Science 123:309–14.
::::* Warburg O (1956) On the origin of cancer cells. Science 123:309–14.
* [[Fang 2010 Cell|Fang M, Shen Z, Huang S, Zhao L, Chen S, Mak TW, Wang X (2010) The ER UDPase ENTPD5 promotes protein N-glycosylation, the Warburg effect, and proliferation in the PTEN pathway. Cell 143:711-24]].  
::::* [[Fang 2010 Cell|Fang M, Shen Z, Huang S, Zhao L, Chen S, Mak TW, Wang X (2010) The ER UDPase ENTPD5 promotes protein N-glycosylation, the Warburg effect, and proliferation in the PTEN pathway. Cell 143:711-24]].  
::Warburg effect: "elevation of aerobic glycolysis seen in tumor cells"; "elevated lactate production under aerobic conditions".
:::: Warburg effect: "elevation of aerobic glycolysis seen in tumor cells"; "elevated lactate production under aerobic conditions".


* [[Vaupel MiP2010 |Vaupel P, Mayer A (2010) Evidence against a mitochondrial dysfunction in cancer cells as a hallmark of malignant growth. Mitochondr Physiol Network 15.06:79.]]
::::* [[Vaupel MiP2010 |Vaupel P, Mayer A (2010) Evidence against a mitochondrial dysfunction in cancer cells as a hallmark of malignant growth. Mitochondr Physiol Network 15.06:79.]]


* [[Van Beek MiP2010 |van Beek JHGM (2010) Computational model predictions of metabolic fluxes connecting cytosol and mitochondrial matrix under ‘Warburg effect’ conditions. Mitochondr Physiol Network 15.06:80.]]
::::* [[Van Beek MiP2010 |van Beek JHGM (2010) Computational model predictions of metabolic fluxes connecting cytosol and mitochondrial matrix under ‘Warburg effect’ conditions. Mitochondr Physiol Network 15.06:80.]]

Revision as of 20:57, 8 September 2018


high-resolution terminology - matching measurements at high-resolution


Warburg effect

Description

Requires definition


Reference: http://en.wikipedia.org/wiki/Warburg_effect


MitoPedia methods: Respirometry 



Oxygen and pH - Warburg versus Crabtree Effect

Q: For quantification of aerobic glycolysis in intact cells, the measurement of proton production can be used as an indirect but continuous record of lactate production and corresponding acidification of the medium, while simultaneously monitoring oxygen concentration and oxygen consumption (MultiSensor-O2k). Is this related to the Warburg or Crabtree effect?
A: Under various metabolic conditions, lactic acid production is the dominant mechanism causing acidification, hence the pH measurement is a good indirect indicator of aerobic glycolysis. An early paper summarizing the literature in this field states:
"At high fructose concen­trations, respiration is inhibited while glycolytic end products accumulate, a phenomenon known as the Crabtree effect. It is commonly believed that this effect is restric­ted to microbial and tumour cells with uniquely high glycolytic capaci­ties (Sussman et al, 1980). How­ever, inhibition of respiration and increase of lactate production are observed under aerobic condi­tions in beating rat heart cell cultures (Frelin et al, 1974) and in isolated rat lung cells (Ayuso-Parrilla et al, 1978). Thus, the same general mechanisms respon­sible for the integra­tion of respiration and glycolysis in tumour cells (Sussman et al, 1980) appear to be operating to some extent in several isolated mammalian cells." Gnaiger 1990 Biochim Biophys Acta


From the literature

Warburg effect: "elevation of aerobic glycolysis seen in tumor cells"; "elevated lactate production under aerobic conditions".