Difference between revisions of "MitoEAGLE data: muscle"
From Bioblast
Line 74: | Line 74: | ||
=== TG2.1 Skeletal === | === TG2.1 Skeletal === | ||
:: TG leaders: [[Garcia-Roves Pablo M]] / [[Votion Dominique-Marie]] / [[Coen Paul M]] | :: TG leaders: [[Garcia-Roves Pablo M]] / [[Votion Dominique-Marie]] / [[Coen Paul M]] | ||
:: TG participants: [[Boyle John P]], [[Chabi Beatrice]], [[Garcia-Roves Pablo M]], [[Lehti Maarit]], [[Rustan Arild]], [[Schlattner Uwe]], [[Wuest Rob C]] | :: TG participants: [[Boyle John P]], [[Chabi Beatrice]], [[Garcia-Roves Pablo M]], [[Lehti Maarit]], [[Mars Tomaz]], [[Pirkmajer Sergej]], [[Rustan Arild]], [[Schlattner Uwe]], [[Wuest Rob C]] | ||
Revision as of 11:08, 16 September 2016
News and Events | Working Groups | Short-Term Scientific Missions | Management Committee | Members |
COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: muscle
WG2
- MITOEAGLE data repository in muscle and other tissues
WG2 Project application
Description
- During the past 10 years, a dramatically increasing number of studies report respirometric data on human muscle tissue (e.g. Christiansen 2015 Am J Physiol). Muscle mitochondrial function is particularly studied in genetically engineered mouse models of human diseases (e.g. Jelenik 2014 Diabetes). However, despite the many published data, lack of consistency often precludes quantitative comparison among data sets with permeabilized or homogenized muscle fibres or isolated mitochondria, normalized to either tissue wet weight, dry weight, protein content, citrate synthase activity or other mitochondrial markers. In the absence of validated conversion factors, these data remain largely unconnected and can only be qualitatively linked.
- The aim of WG2 will be to provide access to complete data sets for post-study statistical analysis and use harmonization tools (WG1) for standardized documentation, to initiate a data repository on muscle tissue from humans and model organisms in health and disease. Data sets shall be obtained from already published and new studies, prompting participating labs to report experimental details beyond those already published as far as possible (using tools such as PubMed Commons). As a result, specific protocols will be summarized for handling muscle tissue. Depending on specific interests, WG2 may decide to extend their focus to other tissue types.
Management
Tasks
- Call for participating labs to deliver relevant experimental data on already published studies and to report them in a standardized format.
- Collection and indexing of the data sets as required for statistical analysis.
- Discussion of procedures, protocols and general guidelines.
- Draft a comprehensive review on mt-function in muscle tissue (registration at PROSPERO, link with EQUATOR).
- Opening of the database to the research public for data search and moderated entry of new data sets.
Milestones
- Call for data report delivered.
- Data sets entered in standardized format.
- Summary, interpretation and discussion of data for review.
Deliverables
- A MITOEAGLE database on muscle tissue from humans and model organisms.
- A set of guidelines for future studies on mitochondrial bioenergetics in muscle tissue.
- Draft of review manuscript.
WG2 Action
WG Leader
Participants
Country | City | Contact |
---|---|---|
Australia | Melbourne | Bishop David J |
Austria | Innsbruck | Doerrier Carolina |
Austria | Innsbruck | Laner Verena |
Austria | Innsbruck | Gnaiger Erich |
Austria | Salzburg | Mayr JA |
Belgium | Liege | Votion Dominique-Marie |
Belgium | Leuven | Bird Matthew |
Brazil | Jaboticabal, SP | Garcia Geovana S |
Brazil | São Paulo | Ferreira Julio Cesar B |
Canada | Rimouski | Vandermeeren E |
Canada | Rimouski | Blier Pierre U |
Canada | Vancouver | Boushel Robert C |
Canada | Edmonton (Alberta) | Lemieux Helene |
Canada | Antigonish | Kane Daniel A |
Croatia | Zagreb | Saric A |
Croatia | Split | Mihanovic I |
Czech Republic | Pilsen | Kuncova J |
Czech Republic | Hradec Králové | Waldauf P |
Czech Republic | Prague | Elkalaf Moustafa |
Czech Republic | Pilsen | Markova Michaela |
Czech Republic | Prague 10 | Krajcova Adela |
Denmark | Hvidovre | Nehlin Jan O |
Denmark | Copenhagen | Gonzalez-Franquesa Alba |
Denmark | Malov | Hey-Mogensen M |
Denmark | Copenhagen | Dela Flemming |
Denmark | Copenhagen N | Mortensen OH |
Denmark | Copenhagen | Larsen Steen |
Estonia | Tallinn | Puurand Marju |
Estonia | Tallinn | Truu Laura |
Estonia | Tallinn | Aid-Vanakova Jekaterina |
Estonia | Tallinn | Kaambre Tuuli |
Estonia | Tallinn | Karro N |
Estonia | Tallinn | Vendelin Marko |
Estonia | Tallinn | Tepp Kersti |
Estonia | Tartu | Paju Kalju |
Finland | Helsinki | Pirinen E |
Finland | Jyvaeskylae | Lehti Maarit |
France | Paris | Armand Anne-Sophie |
France | Paris | Petit Patrice X |
France | Bordeaux cedex | Mourier A |
France | Grenoble cedex 9 | Dubouchaud Herve |
France | Grenoble Cedex 9 | Schlattner Uwe |
France | Montpellier Cedex 1 | Chabi Beatrice |
France | Montpellier | Cortade F |
France | Paris | Ventura-Clapier R |
Germany | Munich | Jastroch M |
Germany | Bochum | Platen P |
Germany | Köln | Kimoloi Sammy |
Germany | Düsseldorf | Granata Cesare |
Germany | Duesseldorf | Jelenik T |
Germany | Bremerhaven | Mark Felix Christopher |
Germany | Duesseldorf | Roden Michael |
Germany | Leipzig | Ost Mario |
Germany | Rostock | Haider F |
Germany | Cologne | Pesta Dominik |
Germany | Nuthetal | Klaus S |
Greece | Heraklio | Sidiropoulou K |
Greece | Athens | Trougakos Ioannis P |
Greece | Heraklion | Tavernarakis Nektarios |
India | Haryana | Sharma Vipin |
Ireland | Dublin | Porter Richard K |
Ireland | Sligo | Murphy J |
Ireland | Dublin | O'Gorman Donal |
Israel | Rishon Le Zion | Hachmo Yafit |
Israel | Zrifin | Sova Marina |
Israel | Jerusalem | Saada Reisch Ann |
Italia | Udine | Salvadego Desy |
Italy | Milan | De Palma Clara |
Italy | Milan | Giovarelli Matteo |
Italy | Bologna | Genova Maria Luisa |
Italy | Pisa | Gastaldelli Amalia |
Italy | Novara | Reano Simone |
Italy | Rome | Scatena Roberto |
Japan | Sapporo | Shirakawa R |
Japan | Sapporo | Nambu H |
Japan | Sapporo | Maekawa S |
Latvia | Riga | Liepins Edgars |
Latvia | Riga | Kuka Janis |
Latvia | Riga | Zvejniece Liga |
Latvia | Riga | Inashkina I |
Luxembourg | Strassen | Devaux Yvan |
Malta | Msida | Vella Joanna |
Netherlands | Amsterdam | De Goede Paul |
Netherlands | BT Amsterdam | Jaspers Richard T |
Netherlands | Maastricht | Grevendonk L |
Netherlands | Maastricht | Hoeks Joris |
Netherlands | Maastricht | Van Polanen N |
Netherlands | Wageningen | Keijer Jaap |
Niederlande | ER Masstricht | De Wit-Verheggen V |
Niederlande | ER Masstricht | De Theije C |
Norway | Tromsoe | Larsen Terje S |
Norway | Tromsoe | Aasum E |
Norway | Oslo | Torp May-Kristin |
Norway | Oslo | Nikolic N |
Norway | Tromsoe | Jansen Kirsten M |
Norway | Oslo | Rustan AC |
Norway | Bergen | Tronstad Karl Johan |
Norway | Tromsoe | Schots Pauke C |
Norway | Ullevål | Heiestad Christina |
Norway | Oslo | Eide L |
Norway | Oslo | Lund Jenny |
Poland | Warsaw | Dymkowska Dorota |
Poland | Lodz | Watala Cezary |
Poland | Lodz | Labieniec-Watala Magdalena |
Poland | Warsaw | Sek Aleksandra |
Poland | Warsaw | Kampa Rafal Pawel |
Portugal | Coimbra | Pereira Susana P |
Portugal | Coimbra | Carvalho Eugenia |
Portugal | Porto | Beleza Jorge |
Portugal | Cantanhede | Martins Joao D |
Portugal | Cantanhede | Ferreira LL |
Portugal | Coimbra | Martins Marques T |
Portugal | Porto | Coxito Pedro |
Portugal | Coimbra | Palmeira Carlos |
Portugal | Porto | Magalhaes Jose |
Romania | Timisoara | Muntean Danina M |
Romania | Timisoara | Lighezan R |
Singapore | Singapore | Hausenloy Derek J |
Slovakia | Bratislava | Nemec Michal |
Slovakia | Bratislava | Ukropec J |
Slovakia | Bratislava | Sumbalova Zuzana |
Slovakia | Bratislava | Ukropcova B |
Slovakia | Bratislava | Maderova D |
Slovenia | Ljubljana | Dolinar K |
Slovenia | Ljubljana | Mars T |
Slovenia | Ljubljana | Pirkmajer Sergej |
South Africa | Smith James | |
Spain | Barcelona | Batlle M |
Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
Spain | Barcelona | Catalan M |
Spain | Barcelona | Zorzano Antonio |
Spain | Barcelona | Garcia-Roves Pablo Miguel |
Spain | Armilla (Granada) | Acuna-Castroviejo Dario |
Spain | Barcelona | Gama Perez Pau |
Spain | A Coruña | Blanco FJ |
Spain | Barcelona | Guitart M |
Spain | Armilla, Granada | Escames Germaine |
Spain | Granada | Lopez Garcia Luis Carlos |
Spain | Madrid | Moran M |
Spain | Barcelona | De la Torre Lara J |
Spain | Sevilla | Bustos Matilde |
Spain | Barcelona | Sebastian D |
Spain | Madrid | Cadenas S |
Sweden | Linköping | Fontes-Oliveira CC |
Sweden | Stockholm | Cardinale Daniele A |
Switzerland | Bern | Nuoffer Jean-Marc |
Switzerland | Lausanne | Morato Fornaguera L |
Switzerland | Lausanne | Sandi Carmen |
Switzerland | Lausanne | Weger M |
Switzerland | Lausanne | Zalachoras I |
Switzerland | Lausanne | Gebara E |
The Netherlands | Wageningen | Van der Ende Miranda |
The Netherlands | BT Amsterdam | Wuest Rob CI |
Turkey | Ankara | Tuncay Erkan |
USA | Baltimore | Fang Evandro F |
USA | Colorado Springs | Jacobs RA |
USA | Boca Raton | Khamoui Andy V |
USA | Norfolk | Lai Nicola |
USA | Ann Arbor | Beard Daniel A |
USA | Gainesville | Hepple Russell T |
USA | Baton Rouge | Axelrod Christopher L |
USA | Orlando | Distefano Giovanna |
USA | St Louis | Welch R |
USA | Gainesville | Neyroud D |
USA | College Station | Latham C |
USA | Chicago | Dayanidhi Sudarshan |
USA | Little Rock | Porter Craig |
USA | Fairbanks | O'Brien Kristin |
USA | Gainesville | Wohlgemuth Stephanie E |
USA | Baltimore | Gonzalez-Freire Marta |
USA | Orlando | Coen Paul M |
USA | College Station | White-Springer Sarah H |
USA | Torrance | Rossiter Harry B |
USA | Baton Rouge | Irving Brian A |
USA | Fort Collins | Chicco Adam J |
United Kingdom | Birmingham | Ashcroft S |
United Kingdom | Leeds | Boyle JP |
United Kingdom | Oxford | Makrecka-Kuka Marina |
United Kingdom | Birmingham | Joanisse S |
United Kingdom | Manchester | Giakoumaki I |
United Kingdom | Loughborough | Ebanks Brad |
United Kingdom | Plymouth, Devon | McKenna Helen T |
United Kingdom | London | Szabadkai Gyorgy |
United Kingdom | Cambridge | Shiroma Jonathan G |
United Kingdom | Brighton | Moore Anthony L |
United Kingdom | Birmingham | Thakker A |
United Kingdom | Birmingham | Garten Antje |
United Kingdom | Cambridge | Murray Andrew James |
United Kingdom | Nottingham | Chakrabarti Lisa |
United States | Providence | Siamwala J |
Task groups
- TG leaders: suggestions discussed at the MitoFit Science Camp 2016 Kuehtai AT.
- TG participants: listed as a summary from the feedback received.
TG2.1 Skeletal
- TG leaders: Garcia-Roves Pablo M / Votion Dominique-Marie / Coen Paul M
- TG participants: Boyle John P, Chabi Beatrice, Garcia-Roves Pablo M, Lehti Maarit, Mars Tomaz, Pirkmajer Sergej, Rustan Arild, Schlattner Uwe, Wuest Rob C
TG2.2 Cardiac
- TG leaders: Larsen Terje S / Makrecka-Kuka Marina
- TG participants: Muntean Danina M, Schlattner Uwe, Vendelin Marko, Wuest Rob C
Comments and discussion
- Skeletal muscle is the main tissue we are studying in the lab. - Beatrice Chabi (2016).
- For the kind of work group, our expertise is certainly most on muscle tissue (skeletal and heart), although we also worked on all kinds of permeabilized cells (primary cells and cell lines). For all these, we have extended data sets, mainly rat, but also human, from diverse experimental setups (nutritional regimen, exercise, drugs ...). - Uwe Schlattner (2016).
- In my working group we are mainly interested in muscle, especially focusing on aging and sarcopenia (using animal models). I would be happy if new cooperations could be formed through the COST Action. I could imagine working on a joint review article. Next to examining muscle from mouse models, we are studying biopsies from human heart muscle and liver, but there is no published data on these studies yet. - Susanne Klaus (2016). Translated by Sandra Fleischmann
- I am working in the field of mitochondrial biochemistry since many years. In detail my coworkers and I are carrying out studies on the regulation of ATP synthase and respiratory complexes in swine heart mitochondria as biological model of mammalian mitochondria. Some comparative aspects of mitochondrial bioenergetics and ATP synthase responses are also investigated in bivalve mollusk mitochondria. - Alessandra Pagliarani (2016).
- We are mostly interested in two WG: (1) Since we are working mostly with heart and muscles, we are interested in WG 2 - MITOEAGLE data repository in muscle and other tissues. (2) WG 1 - Standard operating procedures and user requirement document: Protocols, terminology, documentation. Since we have an expertise in fatty acid metabolism, we are specially interested in experimental protocols for the evaluation of mitochondrial capacities regarding fatty acid metabolism. Of course our parts of the WG are also in our interest. - Marina Makrecka-Kuka, Edgars Liepinsh (2016).
- My role in Mito-EAGLE would be mainly in WG 2 and 4, with a focus on skeletal and cardiac muscle and cultured cardiomyocytes and endothelial cells. I would particularly like to bridge the gap between the group leaders and young investigators, trying to represent the young people who are the active users of the OROBOROS equipment. This will fit in nicely with the goals in WG1. ... Major congratulations on obtaining this beautiful collaborative grant! Very glad to see this network getting the recognition it deserves! Looking forward to being part of this! - Rob C Wuest (2016).
- We mostly work with cultured human skeletal muscle cells, from different donor groups such as lean, obese, type 2 diabetics, as well as muscle cells from athletes. We are interested in collaboration with our cells and measurements on energy metabolism and mitochondral function. - Arild Rustan (2016).
- Please, find enclosed my declaration to participate with my team in the following workgroups: WG1, WG2, WG3, WG4. We are now involved and have been in not a distant past – in studying of the following cell /tissue types:
- Blood cells, with particular emphasis on platelets and lymphocytes – isolated from animals and humans.
- Endothelial cells, fibroblasts, neurocytes (neuroblastoma), astrocytes, cancer cell lines – predominantly cultured.
- Hepatocytes, cardiomyocytes, cell isolated from brain – isolated from lab animals. - Cezary Watala (2016).
- I have already contacted the portuguese CNC to nominate me for the MC of theaction. Concerning my active participation, taking into account the type of work we do in my lab I could be included in all WGs. As you know we work with mitochondria from liver, muscle and fat, as well as cultured cells from different origins (WG2-4). The elaboration of SOPs (WG1) we can certainly contribute (see the book I edited on Mitochondrial Bioenergetics and in which you contribute a chapter). I believe that everybody can be part of the WG5, but as the coordinators of the action, you and Sandra should decide what is best for the success of the action. - Carlos Palmeira (2016).
- As you know, I have received the MC nomination from our CNC in Bucharest (Ms Elena Dinu) for my colleague, Rodica Lighezan and myself. My expertise is most on muscle tissue (in particular, heart) and also, liver - so I will go for WG2 if this is fine with you. However, Both my colleague and I are mostly interested in performing respirometry in blood cells (and this is what we will start to try in the near future); thus, you decide whether we can fit both of us in WG4, or only her. Also, in order to extend the Network in other Romanian cities with powerful state medical universities, Cluj-Napoca (as you know) and Tg Mures - 2 young men, Bela Kiss and Ovidiu Cotoi have been proposed as substitute members (for gender balance too). In particular, we want to jointly ”explore” the exciting fields of mitochondrial ”hidden toxicity” and dynamics, respectively since the former colleague is a toxicologist and the latter a pathologist. I wish you good luck and I am indeed happy and proud to be part of this growing network. - Danina M Muntean (2016).
- I took a look to the MoU and the different working groups. You know well my research focus and having this into account I am interested in actions related to Working group 1 (Respirometric reference protocols, Interlaboratory proficiency test, Instrumental platforms). Working group 2 and 3: I have generated multiple mitochondrial respiration data sets in different mouse tissues (mainly in skeletal muscle, hypothalamus, liver and white adipose tissue). Currently we are also performing these assays in white adipose tissue from human patients. Hence I am interested in all milestones of WG 2 and 3. WG 5 is related to WG 1 and as you know I have interest in training, dissemination and also together with my collaborators (if needed) we could help with data sets and a data interpretation. Overall, I have a general interest on this Action and my active role in MITOEAGLE could be discussed. I could be of help participating in some of the tasks where my expertise and interest fit best. We will be forming and interdisciplinary research group with four group leaders: JC Perales, R Guimera, M Sales-Pardo and PM Garcia-Roves with two different affiliations, University of Barcelona (JCP and PMG-R - Energy metabolism) and University Rovira I Virgili (RG and MS – Data base, statistics, data mining). - Pablo M Garcia-Roves (2016).
- Congratulations on the considerable progress with MITOEAGLE! I am keen to participate in this exciting project and I’m sure I can make a valuable contribution to it's success. I have extensive experience performing respirometry assays in human muscle biopsies, myoblasts/tubes and mouse tissues in the contexts of aging, obesity, exercise and inactivity. I feel that I could contribute to any of the five working groups, but particularly to WG2, WG4, and WG5. I am also happy and willing to serve on the Management Committee if needed. Again, congratulations on organizing such an exciting project. - Paul M Coen (2016).
- In particular, my team can participate with studies about the mitochondrial function in relation to the supramolecular organization of the respiratory chain where the dynamic presence of the respiratory supercomplexes is a way to shift between the main metabolic fluxes of NAD- and FAD-dependent substrates in different metabolic conditions of the cell. Besides my basic studies on the enzyme interactions with the Coenzyme Q pool, I recently joined an Italian consortium of University laboratories whose target is to shed light on the molecular mechanisms underpinning muscle weakness and reduced muscle mass (sarcopenia) in ageing and attenuated response to exercise training stimuli in the elderly; both animal models and human subjects will be analyzed. - Maria Luisa Genova (2016).
- I would be interested to be involved with WG1 and WG2. In particular, I am interested in contributing to developing protocols for multicenter quality control among labs, and for permeabilized muscle bundle respirometry. - Harry B Rossiter (2016).
- Our main research subject is the heart muscle cells. So, we could contribute in datasets for several animals (rat, trout, mice [WT and KOs]). In collaboration with WG1, it would be possible to report data in standardized format. - Marko Vendelin (2016)
Projects in progress
- » MiPMap