Difference between revisions of "MitoEAGLE protocols, terminology, documentation"
From Bioblast
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=== TG1.1 Coupling states and coupling control ratios: Consensus statements === | === TG1.1 Coupling states and coupling control ratios: Consensus statements === | ||
:: TG leaders: [[Renner-Sattler Kathrin]] / [[Gnaiger Erich]] / [[Hoppel Charles L]] | :: TG leaders: [[Renner-Sattler Kathrin]] DE / [[Gnaiger Erich]] AT / [[Hoppel Charles L]] US | ||
:: TG participants: | :: TG participants: | ||
=== TG1.2 SUIT protocols: nomenclature consistent with concept of database === | === TG1.2 SUIT protocols: nomenclature consistent with concept of database === | ||
:: TG leader: [[Gnaiger Erich]] | :: TG leader: [[Doerrier Velasco Carolina A]] AT, [[Gnaiger Erich]] AT | ||
:: TG participants: [[Attimonelli Marcella]], [[Krako Nina]] | :: TG participants: [[Attimonelli Marcella]] IT, [[Krako Nina]] RS | ||
=== TG1.3 Experimental details in SUIT === | === TG1.3 Experimental details in SUIT === | ||
:: TG leaders: [[Chicco Adam J]] / [[Hickey Anthony J]] / [[Doerrier Velasco Carolina A]] | :: TG leaders: [[Chicco Adam J]] US/ [[Hickey Anthony J]] NZ/ [[Doerrier Velasco Carolina A]] AT | ||
:: TG participants: [[Casado Pinna Marta]], [[Krako Nina]] | :: TG participants: [[Casado Pinna Marta]] ES, [[Krako Nina]] RS | ||
=== TG1.4 Instrumental platforms: comparisons === | === TG1.4 Instrumental platforms: comparisons === | ||
:: TG leaders: [[Molina Anthony JA]] | :: TG leaders: [[Elmer Eskil]] SE (suggested), [[Molina Anthony JA]] US | ||
:: TG participants: [[Canto Carles]] (suggested), [[Genova Maria Luisa]], [[Tronstad Karl Johan]] (suggested), [[Zorzano Antonio]] (suggested) | :: TG participants: [[Canto Carles]] CH (suggested), [[Genova Maria Luisa]] IT, [[Tronstad Karl Johan]] NO (suggested), [[Zorzano Antonio]] ES (suggested) | ||
=== TG1.5 Knowledge management system === | === TG1.5 Knowledge management system === | ||
:: TG leaders: [[Drinnan Michael]] / [[Plattner Christina]] | :: TG leaders: [[Drinnan Michael]] UK/ [[Plattner Christina]] AT | ||
:: TG participants: | :: TG participants: | ||
=== TG1.6 Proficiency test === | === TG1.6 Proficiency test === | ||
:: TG leaders: [[Gnaiger Erich]] / [[Doerrier Velasco Carolina A]] | :: TG leaders: [[Gnaiger Erich]] AT/ [[Doerrier Velasco Carolina A]] AT | ||
:: TG participants: [[Genova Maria Luisa]], [[Plattner Christina]] | :: TG participants: [[Genova Maria Luisa]] IT, [[Plattner Christina]] AT | ||
=== TG1.7 Documentation === | === TG1.7 Documentation === | ||
:: TG leaders: [[Arnould Thierry]] / [[Votion Dominique-Marie]] | :: TG leaders: [[Arnould Thierry]] BE/ [[Votion Dominique-Marie]] BE | ||
:: TG participants: | :: TG participants: | ||
=== TG1.8 Translation to community in general === | === TG1.8 Translation to community in general === | ||
:: TG leader: [[Irving Brian A]] | :: TG leader: [[Irving Brian A]] US | ||
:: TG participants: [[Krako Nina]] | :: TG participants: [[Krako Nina]] RS | ||
=== TG1.9 Dissemination Scientific community (formally WG5) === | === TG1.9 Dissemination Scientific community (formally WG5) === | ||
:: TG leaders: [[ | :: TG leaders: [[Makrecka-Kuka Marina]] LV / [[Gnaiger Erich]] AT | ||
:: TG participants: [[Ali Sameh S]], [[Cervinkova Zuzana]], [[Chicco Adam J]], [[Coen Paul M]], [[Eide Lars]], [[Garcia-Roves Pablo M]], [[Gasparre Giuseppe]], [[Genova Maria Luisa]], [[Haendeler Judith]], [[Iyer Shilpa]], [[Krako Nina]], [[Mayan Maria D]], [[Mishmar Dan]], [[Nehlin Jan]], [[Palmeira Carlos]], [[Paterlini Serena]], [[Porcelli Anna Maria]], [[Rao Raj]], [[Schaefer Patrick]], [[Tronstad Karl Johan]], [[Trougakos Ioannis]], [[Volani Chiara]], [[Votion Dominique-Marie]] | :: TG participants: [[Ali Sameh S]] EG, [[Cervinkova Zuzana]] CZ, [[Chicco Adam J]] US, [[Coen Paul M]] US, [[Eide Lars]] NO, [[Garcia-Roves Pablo M]] ES, [[Gasparre Giuseppe]] IT, [[Genova Maria Luisa]] IT, [[Haendeler Judith]] DE, [[Iyer Shilpa]] US, [[Krako Nina]] RS, [[Mayan Maria D]] ES, [[Mishmar Dan]] IL, [[Nehlin Jan]] DK, [[Palmeira Carlos]] ES, [[Paterlini Serena]] IT, [[Porcelli Anna Maria]] IT, [[Rao Raj]] US, [[Schaefer Patrick]] DE, [[Tronstad Karl Johan]] NO, [[Trougakos Ioannis]] GR, [[Volani Chiara]] AT, [[Votion Dominique-Marie]] BE | ||
=== TG1.10 Training, Short Term Scientific Missions (formally WG5) === | === TG1.10 Training, Short Term Scientific Missions (formally WG5) === | ||
:: TG leader: [[Klaus Susanne]] (suggested), [[Labieniec-Watala Magdalena]] (suggested) | :: TG leader: [[Klaus Susanne]] DE (suggested), [[Labieniec-Watala Magdalena]] PL (suggested) | ||
:: TG participants: [[Ali Sameh S]], [[Cervinkova Zuzana]], [[Chicco Adam J]], [[Coen Paul M]], [[Eide Lars]], [[Garcia-Roves Pablo M]], [[Gasparre Giuseppe]], [[Genova Maria Luisa]], [[Haendeler Judith]], [[Iyer Shilpa]], [[Krako Nina]], [[Mayan Maria D]], [[Mishmar Dan]], [[Nehlin Jan]], [[Palmeira Carlos]], [[Paterlini Serena]], [[Porcelli Anna Maria]], [[Rao Raj]], [[Schaefer Patrick]], [[Tronstad Karl Johan]], [[Trougakos Ioannis]], [[Volani Chiara]], [[Votion Dominique-Marie]] | :: TG participants: [[Ali Sameh S]] EG, [[Cervinkova Zuzana]] CZ, [[Chicco Adam J]] US, [[Coen Paul M]] US, [[Eide Lars]] NO, [[Garcia-Roves Pablo M]] ES, [[Gasparre Giuseppe]] IT, [[Genova Maria Luisa]] IT, [[Haendeler Judith]] DE, [[Iyer Shilpa]] US, [[Krako Nina]] RS, [[Mayan Maria D]] ES, [[Mishmar Dan]] IL, [[Nehlin Jan]] DK, [[Palmeira Carlos]] ES, [[Paterlini Serena]] IT, [[Porcelli Anna Maria]] IT, [[Rao Raj]] US, [[Schaefer Patrick]] DE, [[Tronstad Karl Johan]] NO, [[Trougakos Ioannis]] GR, [[Volani Chiara]] AT, [[Votion Dominique-Marie]] BE | ||
Revision as of 08:46, 10 September 2016
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News and Events |  |
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Working Groups | Short-Term Scientific Missions | Management Committee | Members |  |
COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE protocols, terminology, documentation
WG1
- Standard operating procedures and user requirement document: Protocols, terminology, documentation
WG1 Project application
Description
- The quality management system, scope of SOPs to be elaborated and the outline of the data repositories will be defined in a user requirement document.
Respirometric reference protocols
- A ‘library of protocols’ applied in mitochondrial respiratory physiology will be collected in a standard format, delineating the diversity of experimental approaches to assess bioenergetic function. A set of reference protocols will rigorously document (Maelstrom Research program) the state-of-the-art standards in designing, conducting, reporting, interpreting, and validating such bioenergetic tests (see also figure on the right).
Interlaboratory proficiency test
- Inter-laboratory ring tests are a basic requirement for quality management. Such proficiency tests require a reference sample which must be homogenous, stable, representative of the diagnostic target, amenable for distribution, and economical for large-scale production. For respiratory OXPHOS analysis a reference sample of structurally and functionally intact mitochondria is not yet available to perform corresponding ring tests (in contrast to enzymatic OXPHOS ring tests). Recently, the widely applied human cell line HEK 293T was found to be potentially suitable for cryopreservation. Upon thawing the cells can be used immediately for respirometry of both intact and permeabilized cells. Thus interlaboratory proficiency testing may now be feasible as a world-wide innovation in the field of mitochondrial respiratory physiology. Participating labs may implement the test for intra- and interlaboratory validation and longitudinal performance monitoring, using a reference OXPHOS protocol (Fig. 3). The organization of the proficiency test should follow the requirements of the ISO 17043 and 13528 standards. A corresponding MITOEAGLE proficiency training shall be developed and implemented. Feedback from the participants will lead to a final adjustment of the SOPs in the proficiency test, which will be made generally available.
Instrumental platforms
- Comparison of results between and within instrumental platforms: The reference protocols and proficiency test will not define the instrumental platform, but will allow a quantitative comparison of results obtained with specific equipment available in the participating laboratories.
Nomenclature
- Harmonization of nomenclature on mitochondrial respiratory states and control parameters: The logistics of development of a database requires application of strictly defined terms for all included variables. There is no general reference available upon which a consistent terminology on mitochondrial physiology and bioenergetics can be based. The MITOEAGLE consortium, therefore, has to accomplish the ambitious goal to unify and simplify the terminology in the field for the purpose of the QMS, which will lead to the development of recommendations for the use of a common terminology in mitochondrial physiological research. A publication will be prepared as an Open Access article which will be a milestone towards a unification of concepts and nomenclature.
Knowledge management system
- With an increase in data volume and complexity comes along the requirement for standardized report formats and digital accessibility of the data. Since such a database is still missing for mitochondrial physiology, the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. Databases will be established combining biological and clinical data comprising proprietary and publicly available datasets into one integrated solution. Data import and export functionalities will allow data upload and mapping to corresponding objects in the database, in this way generating valuable data and allowing for project-specific as well as cross-project data mining.
- The ability to formulate meta-studies in our approach is an important success factor: The platform can aggregate the project’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases. In addition to functional data, other data types such as nutritional or environmental factors associated with disease prevention can be identified and aggregated into the MITOEAGLE Knowledge Management System.
- Querying the heterogeneous data types and result representation are the most important requirements of the knowledge platform besides to data integration and modelling. The application will feature different types of visualization techniques for all integrated data types. The database will be developed using the concept of multi-tier client-server architecture. Individual components are separated corresponding to their functionality. An upstream web server handles user requests, an application server performing business logic, and a back-end database system guaranties persistent data storage. All components are additionally separated by means of network security incorporating firewalls and are hosted in a secure environment. The platform administration ensures privacy regarding proprietary data by user rights policies.
- It will be the task of WG1 to continuously collect the input from all other WGs, compare, discuss and align SOPs as far as possible, and to finally develop recommendations for quality control, data reporting and data sharing beyond the published record. In addition, WG1 will develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation. Finally, it will provide a summary on strategic dissemination and an education programme for MITOEAGLE. The education programme will ensure that not only presently participating groups will adhere to the set standards but that also groups joining in at later points may smoothly fit into the community and help to further broaden and deepen our understanding on mitochondrial physiology in health and disease.
Management
Tasks
- Opening of the database to the research public for data search and moderated entry of new datasets.
- Development of interlaboratory proficiency ring test, of recommendation/certification reports and of a MITOEAGLE proficiency training module.
- Sample distribution, ring test experiments (MITOEAGLE-proficiency training on two different points of time, 6 months apart), data analysis by experimenters and MITOEAGLE-proficiency test manager.
- Reports and publication: Reports with specific results in comparison with an anonymous data summary, and joint publication. Individual reports may be complemented by recommendations, and are summarized in an informal certification report.
- Instrumental platform comparison with reference sample applying comparable measurements.
- Draft, dissemination, collection and final formulation of a manuscript on concepts and terminology of mitochondrial physiology.
- Collect and discuss procedures and experimental protocols for the evaluation of mitochondrial capacities and create a library of protocols: A database comprising reference SUIT protocols, standard experimental media, and detailed instructions for OXPHOS analysis is assembled.
- MITOEAGLE data management system (DMS) development: The DMS for further data preprocessing. The DMS will enable management and analysis of instrument-specific and project-specific data.
- MITOEAGLE database (DB) development: DB will facilitate high-level project specific analysis as well as meta-analyses across projects, visualization of project specific data as well as integrative analyses of datasets for various proprietary and/or publicly available datasets.
- Testing of developed software components regarding functionality and usability, modification and implementation. Deployment for routine testing in the labs of our strategic partners.
- Data input: Upload of datasets generated through WG 2-4 in internal lab developments, results of the proficiency ring test, and during application module developments. In addition, the database will be populated with available proprietary and public datasets.
- Develop recommendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation.
- Provide a summary on strategic dissemination and an education programme for MITOEAGLE.
Milestones
- Data sets entered in standardized format and database opened for the public
- Protocols for ring-tests and standard analysis and report scheme defined
- MITOEAGLE proficiency training workshop finished
- Analysis is completed and joint publication submitted
- Publication of paper on concepts and terminology
- Library of protocols online
- Publication of recommended protocols and procedures
- Guidelines for acquisition, evaluation and data documentation available
- MITOEAGLE data management system finished
- MITOEAGLE database ready for use
- Software modifications incorporated following extensive tests
- Publication of MITOEAGLE-KMP
- Recommendations on data acquisition, evaluation and management published
- Concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation published
Deliverables
- Guidelines for future research and recommendations for the evaluation of respiratory characteristics in human respirometric samples
- A database on mt fitness evaluated with human cells and tissues
- Training of researchers towards improved reproducibility of sample preparation and respirometric evaluation
- Qualitative and quantitative evaluation of results obtained in ongoing studies by comparison with a reference sample
- An expanded MITOEAGLE database
- A joint publication documenting the results of the MITOEAGLE-PT
- A multi-authored publication towards a unification of concepts and nomenclature in mitochondrial physiology
- A library of protocols for sample preparation and examination by respirometry
- A multi-authored publication with recommendations of comparable standard protocols and procedures.
- Guidelines as to the optimum use of a reference sample for determination of respiration and related parameters of interest.
- The MITOEAGLE data management system.
- A continuously updated MITOEAGLE database for the use as reference data and guideline towards application of optimized protocols, ultimately leading towards standardization.
- A joint publication on the use of the MITOEAGLE database and management system.
- An education programme for the use of MITOEAGLE database and management system.
WG1 Action
Participants
| Country | City | Contact |
|---|---|---|
| Armenia | Yerevan | Trchounian K |
| Australia | Nedlands | Filipovska Aleksandra |
| Australia | Melobourne | Botella Javier |
| Australia | Southport | Vidimce Josif |
| Australia | Melbourne | Whitfield Jamie |
| Australia | Melbourne, VIC | Muccini Anna Maria |
| Australia | Melbourne | Saner Nicholas |
| Australia | Queensland | Radenkovic Filip |
| Australia | Melbourne | Stepto Nigel K |
| Australia | Newtown, Sydney | Stocker Roland |
| Australia | Geelong, Victoria | McKenzie Matthew |
| Australia | Clayton | Wolff Jonci Nikolai |
| Australia | Syndey | Ton Riccardo |
| Australia | Victoria | Winwood-Smith Hugh |
| Australia | Melbourne | Hewakapuge Sudinna |
| Australia | Queensland | Dong Lan-Feng |
| Australia | Clayton Victoria | Alton Lesley |
| Australia | Melbourne | Ferri Alessandra |
| Australia | Nedlands | Perks Kara L |
| Australia | Victoria | Van Bergen Nicole J |
| Australia | Melbourne | Lionett S |
| Australia | Melbourne | Coughlan Melinda T |
| Australia | Sydney | Hoehn Kyle L |
| Australia | Melbourne | Cassar Samantha |
| Australia | Melbourne | Genders Amanda J |
| Australia | Burwood | Trewin Adam J |
| Australia | Melbourne | Kuang Jujiao |
| Australia | Sydney | Krycer James R |
| Australia | Parkville | Hardee Justin P |
| Australia | Queensland | Fisher Joshua J |
| Australia | Queensland | Holland Olivia J |
| Austria | Innsbruck | Plangger Mario |
| Austria | Innsbruck | Hoppel Florian |
| Austria | Innsbruck | Thapa Maheshwor |
| Austria | Innsbruck | Doerrier Carolina |
| Austria | Innsbruck | Schartner Melanie |
| Austria | Innsbruck | Nindl Veronika |
| Austria | Innsbruck | Goebel G |
| Austria | Innsbruck | Sobotka Ondrej |
| Austria | Innsbruck | Bergmeister Lisa |
| Austria | Innsbruck | Di Marcello Marco |
| Austria | Innsbruck | Antunes Diana |
| Austria | Kapferer Werner | |
| Austria | Innsbruck | Klocker Helmut |
| Austria | Innsbruck | Garcia-Souza Luiz F |
| Austria | Innsbruck | Gallee Leon |
| Austria | Innsbruck | Karabatsiakis Alexander |
| Austria | Innsbruck | Cardoso Luiza HD |
| Austria | Innsbruck | Perez Valencia Juan Alberto |
| Austria | Innsbruck | Schmitt Sabine |
| Austria | Innsbruck | Schwarzer Christoph |
| Austria | Aldrans | Haider Markus |
| Austria | Innsbruck | Laner Verena |
| Austria | Innsbruck | Cecatto Cristiane |
| Austria | Innsbruck | Gnaiger Erich |
| Austria | Klosterneuburg | Sazanov Leonid A |
| Austria | Innsbruck | Meszaros Andras T |
| Austria | Innsbruck | Passrugger Manuela |
| Austria | Salzburg | Kofler B |
| Austria | Vienna | Kozlov Andrey V |
| Austria | Innsbruck | Burtscher Martin |
| Austria | Innsbruck | Keller Markus A |
| Belgium | Namur | Arnould Thierry |
| Belgium | Leuven, Flanders | Baekelandt V |
| Belgium | Brussels | Sonveaux P |
| Belgium | Liege | Votion Dominique-Marie |
| Belguim | Sart Tilman (Liège) | Kruse C |
| Brasil | Porto Alegre | Haas Clarissa |
| Brasilia | Limeira | Ropelle Eduardo R |
| Brazil | Sao Paulo | Vercesi Anibal E |
| Brazil | Rio de Janeiro | Galina Antonio |
| Brazil | Santa Maria - RS | Goncalves Debora Farina |
| Brazil | Sao Paulo | Silber Ariel Mariano |
| Brazil | Santa Maria - Rio Grande do Sul | Soares Felix Alexandre Antunes |
| Brazil | São Paulo | Kowaltowski Alicia J |
| Brazil | Jaboticabal | Oliveira Marcos Tulio |
| Brazil | Rio de Janeiro | Vieyra Adalberto |
| Brazil | Rio de Janeiro | Oliveira Marcus F |
| Brazil | São Paulo | Crispim Marcell |
| Canada | Moncton, NB | Pichaud Nicolas |
| Canada | Saint John | Pulinilkunnil Thomas |
| Canada | Edmonton | Han Woo Hyun |
| Canada | Vancouver, BC | Tausan Daniel |
| Canada | Edmonton (Alberta) | Lemieux Helene |
| Canada | Quebec | Joseph Vincent |
| Canada | Edmonton | Zaugg Michael |
| Canada | Rimouski | Munro Daniel |
| Canada | Charlottetown | Adiele Reginald C |
| Canada | Rimouski | Blier Pierre U |
| Canada | Edmonton | Lucchinetti Eliana |
| Canada | Hamilton | Tarnopolsky Mark A |
| Canada | Antigonish | Weir M |
| Canada | Guelph | Holloway Graham P |
| Canada | Vancouver | Bovard Josh |
| Canada | Ottawa | Darveau Charles-A |
| Canada | Winnipeg | Chowdhury Subir Roy |
| Canada | Montréal | Breton Sophie |
| Canada | Hamilton | Scott Graham R |
| Canada | Montreal, Quebec | Bergdahl Andreas |
| Canada | Ottawa | Harper Mary-Ellen |
| Chile | Coyhaique | Jana Prado Fabian |
| China | Xi`an | Liu Jiankang |
| China | Tianjin | Zhang Yong |
| China | Pukou, Nanjing, | Gan Zhenji |
| China | Fuzhou, Fujian Province | Chen Qi |
| Croatia | Split | Ljubkovic M |
| Croatia | Zagreb | Saric A |
| Croatia | Zagreb | Curik I |
| Croatia | Zagreb | Rubelj Ivica |
| Czech Republic | Hradec Kralove | Kucera Otto |
| Czech Republic | Ceske Budejovice | Zikova Alena |
| Czech Republic | Pardubice | Handl Jiri |
| Czech Republic | Praha 2 | Danhelovska Tereza |
| Czech Republic | Vestec | Ezrova Zuzana |
| Czech Republic | Praha 2 | Zdrazilova Lucie |
| Czech Republic | Vestec | Rohlena Jakub |
| Czech Republic | Hradec Kralove | Stankova Pavla |
| Czech Republic | Hradec Kralove | Cervinkova Zuzana |
| Czech Republic | Vestec | Davidova Eliska |
| Czech Republic | Pardubice | Rousar Tomas |
| Czech Republic | Prague | Drahota Zdenek |
| Czech Republic | Prague 10 | Urban Tomas |
| Czech Republic | Prague | Krizova J |
| Czech Republic | Prague | Houstek Josef |
| Czech Republic | Hradec Kralove | Endlicher Rene |
| Czech Republic | Plzen -Severni predmesti | Jedlicka Jan |
| Czech Republic | Pardubice | Majtnerova Pavlina |
| Czech rep | Hradec Kralove | Falaye T |
| Denmark | Copenhagen | Dela Flemming |
| Denmark | Aarhus N | Boetker Hans Erik |
| Denmark | Copenhagen | Nielsen B |
| Denmark | Hvidovre | Nehlin Jan O |
| Denmark | Copenhagen N | Mortensen OH |
| Denmark | Copenhagen | Larsen Steen |
| Denmark | Copenhagen | Soendergaard SD |
| Denmark | Copenhagen N | Maise Chroeis K |
| Denmark | Copenhagen N | Scheibye-Knudsen Morten |
| Denmark | Copenhagen | Gonzalez-Franquesa Alba |
| Denmark | Copenhagen | Kraunsoee R |
| Denmark | Roskilde | Dalgaard Louise T |
| Denmark | Aarhus N | Jespersen Nichlas Riise |
| Egypt | Cairo | Abdel-Rahman Engy Ali |
| Egypt | Cairo | Ali Sameh S |
| Estonia | Tallinn | Puurand Marju |
| Estonia | Tallinn | Klepinin Aleksandr |
| Estonia | Tallinn | Laasmaa Martin |
| Estonia | Tartu | Paju Kalju |
| Estonia | Tallinn | Kaambre Tuuli |
| Estonia | Tallinn | Karro N |
| Estonia | Tallinn | Shevchuk Igor |
| Estonia | Tallinn | Truu Laura |
| Estonia | Tallinn | Tepp Kersti |
| Estonia | Tallinn | Klepinina Lyudmila |
| Estonia | Tallinn | Vendelin Marko |
| Finland | Turku | Stier Antoine |
| Finland | Helsinki | Jackson Christopher Benjamin |
| Finland | Helsinki | Suomalainen Wartiovaara Anu |
| Finland | Jyvaeskylae | Kainulainen Heikki |
| France | Maisons-Alfort Cedex | Prola Alexandre |
| France | Martinique | Neviere Remi |
| France | Paris | Armand Anne-Sophie |
| France | Paris | Bouillaud Frederic |
| France | Tours | Dumas Jean-Francois |
| France | Plouzané | Salin Karine |
| France | Nouzilly | Collin-Chenot Anne |
| France | Angers | Spinazzi Marco |
| France | Montpellier | Wrutniak-Cabello Chantal |
| France | Lille | Montaigne David |
| France | Martinique | Cano Sanchez Maria Consolacion |
| France | Bordeaux cedex | Mourier A |
| France | Bordeaux | Amoedo Nivea Dias |
| France | Angers | Procaccio Vincent |
| France | Bordeaux cedex | Rossignol Rodrigue |
| France | Gueguen Naig | |
| France | Bordeaux | Sarlak Saharnaz |
| Germany | Ulm | Schaefer PM |
| Germany | Hannover | Das Anibh Martin |
| Germany | Mainz | Methner Axel |
| Germany | Wilhelmshaven | Salmon Pablo |
| Germany | Jena | Szibor Marten |
| Germany | Cologne | Maciej Sarah |
| Germany | Giessen | Sommer Natascha |
| Germany | Munich | Zischka Hans |
| Germany | Duesseldorf | Jelenik T |
| Germany | Frankfurt | Hamann Andrea |
| Germany | Magdeburg | Schoenfeld Peter |
| Germany | Marburg | Vogt Sebastian |
| Germany | Cologne | Wiesner Rudolf J |
| Germany | Wickert Anika | |
| Germany | Cologne | Pesta Dominik |
| Germany | Gießen | Silaidos Carmina |
| Germany | Marburg | Ramzan Rabia |
| Germany | Freiburg | Schuele R |
| Germany | Duesseldorf | Piel Sarah |
| Germany | Cologne | Trifunovic Aleksandra |
| Germany | Duesseldorf | Haendeler Judith |
| Germany | Doermann Niklas | |
| Germany | Frankfurt am Main | Viel Christian |
| Germany | Rostock | Sokolova Inna |
| Germany | Regensburg | Renner-Sattler Kathrin |
| Germany | Bremerhaven | Mark Felix Christopher |
| Germany | Mainz | Maull Felicia |
| Germany | Frankfurt | Osiewacz Heinz D |
| Germany | Neuherberg | Einer C |
| Germany | Biberach an der Riß | Nold V |
| Germany | Frankfurt | Warnsmann Verena |
| Germany | Gießen | Grewal Rekha |
| Germany | Tuebingen | Kappler Lisa |
| Germany | Düsseldorf | Goy C |
| Germany | Giessen | Schulz Rainer |
| Germany | Duesseldorf | Roden Michael |
| Germany | Cologne | Ho Dieu Hien |
| Greece | Athens | Trougakos Ioannis P |
| Greece | Thessaloniki | Lazou Antigone |
| Greece | Heraklion | Tavernarakis Nektarios |
| Greece | Athens | Gumeni Sentiljana |
| Greece | 15771 | Andreadou Ioanna |
| Hungary | Szeged | Ferdinandy Peter |
| Hungary | Budapest | Chinopoulos Christos |
| Hungary | Budapest | Tretter Laszlo |
| Hungary | Budapest | Komlodi Timea |
| Hungary | Szeged | Boros Mihaly |
| Hungary | Budapest | Horvath G |
| Hungary | Debrecen | Verebne Tar K |
| India | Hyderabad | Jha Rajan Kumar |
| India | Lucknow | Gayen Jiaur |
| India | Haryana | Chakrabarti Sasanka |
| India | Varanasi | Sonkar Vijay K |
| India | New Delhi | Thakkar Himani |
| India | Kollam | Suravajhala Prashanth |
| India | New Delhi | Vincent Vinnyfred |
| Iran | Tehran | Safaei Zahra |
| Iran | Tehran | Shirazi Reza |
| Ireland | Dublin | Porter Richard K |
| Ireland | Dublin | O'Gorman Donal |
| Israel | Zrifin | Sova Marina |
| Israel | Jerusalem | Saada Reisch Ann |
| Israel | Beer Sheva | Mishmar Dan |
| Israel | Rishon Le Zion | Hachmo Yafit |
| Italia | Udine | Salvadego Desy |
| Italien | Padova PD | Szabo Ildiko |
| Italy | Catania | Messina Angela |
| Italy | Rome | Scatena Roberto |
| Italy | Milano | Bottoni P |
| Italy | Ancona | Battino Maurizio |
| Italy | Bologna | Paterlini S |
| Italy | Catania | De Pinto Vito |
| Italy | Bologna | Porcelli AM |
| Italy | Padova | Fernandez-Vizarra Erika |
| Italy | St. Lorenzen | Harrison David K |
| Italy | Padova | Viscomi Carlo |
| Italy | Udine | Grassi B |
| Italy | Bolzano | Zanon Alessandra |
| Italy | Padova | Bernardi Paolo |
| Italy | Bologna | Gasparre G |
| Italy | Milan | Clementi Emilio |
| Italy | Bolzano/Bozen | Volani Chiara |
| Italy | Monserrato | Isola Raffaella |
| Italy | Bari | Attimonelli M |
| Italy | Catania | Magri Andrea |
| Italy | Campobasso | Pallotta Maria Luigia |
| Italy | Bologna | Genova Maria Luisa |
| Japan | Itabashi-ku Tokyo | Tanaka Masashi |
| Japan | Sapporo | Matsumoto J |
| Kitzbühel | Innsbruck | Fischer Michael J |
| Korea | Seoul | Pak Youngmi Kim |
| Korea | Incheon | Kwak Hyo Bum |
| Korea (South) | Seoul | Lee Hong Kyu |
| Latvia | Riga | Svalbe Baiba |
| Latvia | Riga | Zvejniece Liga |
| Latvia | Riga | Stelfa Gundega |
| Latvia | Riga | Liepins Edgars |
| Latvia | Riga | Volska Kristine |
| Latvia | Riga | Inashkina I |
| Latvia | Riga | Jansone Baiba |
| Latvia | Riga | Dambrova Maija |
| Latvia | Riga | Vilks Karlis |
| Luxembourg | Strassen | Devaux Yvan |
| Malaysia | Kuala Lumpur | Hassan Hazirah |
| Malta | Msida | Vella Joanna |
| Mexico | Tlalpan | Rodriguez-Enriquez Sara |
| Mexico | Mexico City | Aparicio Trejo OE |
| México | San Pedro Garza García, Nuevo Leon | Garcia-Rivas Gerardo |
| Netherlands | Maastricht | Grevendonk L |
| Netherlands | GA Wageningen | Grefte Sander |
| New Zealand | Auckland | Hickey Anthony J |
| New Zealand | Auckland | Kaur Sarbjot |
| New Zealand | Auckland | Ward Marie Louise |
| New Zealand | Wellington | Berridge Michael V |
| Norway | Trondheim | Rognmo O |
| Norway | Bergen | Pettersen Nitschke Ina Katrine |
| Norway | Bergen | Hoel Fredrik |
| Norway | Bergen | Tronstad Karl Johan |
| Norway | Tromsoe | Boardman Neoma T |
| Norway | Trondheim | Grill V |
| Norway | Bergen | Blindheim Dan Filip |
| Norway | Bergen | Roesland Gro Vatne |
| Norway | Bergen | Berge Rolf K |
| Norway | Bergen | Dyrstad Sissel E |
| Norway | Oslo | Eide L |
| Norway | Bergen | Haavik Jan |
| Norway | Trondheim | Lerfall Joergen |
| Norway | Trondheim | Smenes Benedikte Therese |
| Poland | Gdańsk | Kaczor Jan Jacek |
| Poland | Warsaw | Dymkowska Dorota |
| Poland | Warsaw | Wieckowski Mariusz R |
| Poland | Lodz | Labieniec-Watala Magdalena |
| Poland | Warsaw | Sek Aleksandra |
| Poland | Warsaw | Karkucinska-Wieckowska Agnieszka |
| Poland | Warsaw | Wasilewski M |
| Poland | Warsaw | Szewczyk Adam |
| Poland | Warsaw | Bednarczyk Piotr |
| Poland | Warsaw | Kampa Rafal Pawel |
| Poland | Warszawa | Jaskiewicz Anna |
| Poland | Krakow | Dembinska-Kiec Aldona |
| Poland | Warsaw | Zablocki Krzysztof |
| Poland | Lodz | Watala Cezary |
| Poland | Poznan | Jarmuszkiewicz Wieslawa |
| Portugal | Cantanhede | Amorim Ricardo |
| Portugal | Aveiro | Vitorino Rui Miguel Pinheiro |
| Portugal | Coimbra | Dias Candida |
| Portugal | Porto | Beleza Jorge |
| Portugal | Coimbra | Duarte Filipe Valente |
| Portugal | Porto | Alves Marco G |
| Portugal | Cantanhede | Teodoro Joao Soeiro |
| Portugal | Coimbra | Grilo Luis |
| Portugal | Coimbra | Oliveira Paulo J |
| Portugal | Coimbra | Santos Diana |
| Portugal | Coimbra | Minuzzi Luciele M |
| Portugal | Lisboa | Macedo Maria Paula |
| Portugal | Aveiro | Ferreira Rita Maria P |
| Portugal | Porto | Oliveira Jorge |
| Portugal | Porto | Oliveira Pedro Fontes |
| Portugal | Coimbra | Palmeira Carlos |
| Portugal | Cantanhede | Rodrigues Ana Sofia |
| Portugal | Porto | Crisostomo Luis |
| Portugal | Porto | Moreira Bruno P |
| Portugal | Porto | Silva Ana Maria |
| Portugal | Coimbra | Rolo Anabela Pinto |
| Qatar | Doha | Guarch Meritxell Espino |
| Republic of Ghana | Cape Coast | Tei BN |
| Republic of Korea | Busan | Han Jin |
| Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
| Republic of Korea | Daejeon | Park SH |
| Republic of Korea | Busan | Kim Hyoung Kyu |
| Republic of Serbia | Novi Sad | Andric Silvana |
| Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
| Republic of Serbia | Belgrade | Lalic Nebojsa M |
| Russia | Moscow | Vinogradov Andrey D |
| Russia | Moscow | Pavlova Nadia |
| Russia | Yoshkar-Ola | Dubinin M |
| Serbia | Belgrade | Mandic M |
| Serbia | Belgrade | Djordjevic M |
| Serbia | Belgrade | Misirkic Marjanovic Maja |
| Serbia | Belgrade | Savkovic U |
| Singapore | Singapore | Singh Brijesh Kumar |
| Singapore | Singapore | Hausenloy Derek J |
| Slovakia | Košice | Cizmarova Beata |
| Slovakia | Bratislava | Sumbalova Zuzana |
| Slovakia | Bratislava 45 | Ferko Miroslav |
| Spain | Sevilla | Bustos Matilde |
| Spain | A Coruña | Blanco FJ |
| Spain | Granada | Hidalgo-Gutierrez A |
| Spain | Madrid | Moran M |
| Spain | Valencia | Casado Pinna Marta |
| Spain | Barcelona | Gil J |
| Spain | Barcelona | Gama Perez Pau |
| Spain | Granada | Quiles JL |
| Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
| Spain | Barcelona | Garcia-Roves Pablo Miguel |
| Spain | Barcelona | Perales Jose Carles |
| Spain | Madrid | Hernansanz-Agustin Pablo |
| Spain | Barcelona | De la Torre Lara J |
| Spain | A Coruña | Mayan MD |
| Spain | Barcelona | Zorzano Antonio |
| Spain | Salamanca | Almeida Angeles |
| Spain | Madrid | Rial Eduardo |
| Spain | Madrid | Aragones Lopez J |
| Spain | Las Palmas de Gran Canaria | Calbet Jose AL |
| Spain | Barcelona | Goncalo Teixeira da Silva Rui |
| Spain | Barcelona | Bravo-Sagua Roberto |
| Sweden | Stockholm | Nedergaard J |
| Sweden | Stockholm | Shabalina Irina G |
| Sweden | Stockholm | Larsen Filip J |
| Sweden | Gothenburg | Bhuvanachandran NS |
| Sweden | Lund | Ehinger Johannes K |
| Sweden | Stockholm | Cardinale Daniele A |
| Switzerland | Bern | Wyss RK |
| Switzerland | Basel | Bouitbir Jamal |
| Switzerland | Lausanne | Lagarrigue Sylviane |
| Switzerland | Basel | Fornaro Mara |
| Switzerland | Zurich | Gorr Thomas A |
| Switzerland | Lausanne | Morato Fornaguera L |
| Switzerland | Lausanne | Donnelly Chris |
| Switzerland | Zürich | Zaugg Kathrin |
| Switzerland | Lausanne | Weger M |
| Switzerland | Lausanne | Zanou Nadege |
| Switzerland | Lausanne | Zalachoras I |
| Switzerland | Lausanne | Amati Francesca |
| Switzerland | Lausanne | Canto Alvarez Carles |
| Switzerland | Lausanne | Burtscher Johannes |
| Switzerland | Lausanne | Sandi Carmen |
| Switzerland | Bern | Nuoffer Jean-Marc |
| Switzerland | Lausanne | Gebara E |
| Switzerland | Lausanne | Place Nicolas |
| Taiwan | Changhua City | Wei Yau-Huei |
| The Netherlands | Maastricht | Schrauwen Patrick |
| The Netherlands | Maastricht | Nabben Miranda |
| The Netherlands | BT Amsterdam | Wuest Rob CI |
| The Netherlands | Delft | McMillan Duncan GG |
| The Netherlands | Groningen | Bakker Barbara M |
| UK | Cambridge | Brown Guy C |
| UK | Canterbury, Kent | Gourlay Campbell W |
| UK | London | Lane Nick |
| UK | London | Bettinazzi Stefano |
| UK | London | Rodriguez Enrique |
| USA | Little Rock | Parajuli Nirmala |
| USA | Louisville | Menze Michael A |
| USA | Kansas City | Thyfault John P |
| USA | New York | Galkin Alexander |
| USA | Corvallis | Newsom Sean A |
| USA | Warminster | Orynbayeva Zulfiya |
| USA | Indianapolis | Brozinick Joseph T |
| USA | Columbus | Sims Carrie A |
| USA | Winston-Salem | Gonzalez-Armenta Jenny L |
| USA | Iowa City | Chaurasia Bhagirath |
| USA | Fairbanks | O'Brien Kristin |
| USA | Atlanta | Jang Young Charles |
| USA | Cambridge | Wohlwend Martin |
| USA | Needham | Brown David A |
| USA | St. Louis | Abumrad Nada A |
| USA | Baltimore | Gonzalez-Freire Marta |
| USA | Puskarich Michael A | |
| USA | Miami | Fontanesi F |
| USA | Winston-Salem | Ahn Bumsoo |
| USA | Cleveland | Tandler Bernard |
| USA | Boston | Kristal Bruce S |
| USA | San Diego | Patel Hemal H |
| USA | Stillwater | Davis Michael S |
| USA | Birmingham | Moellering Douglas R |
| USA | Berkeley | Williams Caroline M |
| USA | Akron | Cohen Bruce H |
| USA | Fort Collins | Chicco Adam J |
| USA | East Lansing | Zhang Yizhu |
| USA | Davis | Hellgren Kim T |
| USA | Philadelphia | Ganetzky Rebecca |
| USA | Torrance | Rossiter Harry B |
| USA | Pittsburgh | Jurczak Michael J |
| USA | San Diego; La Jolla | Molina Anthony JA |
| USA | La Jolla | Valentine Joseph Marco |
| USA | Cleveland | Hoppel Charles L |
| USA | St. Louis | Pietka Terri A |
| USA | Orlando | Sparks Lauren M |
| USA | Philadelphia | Jang David H |
| USA | Los Angeles | Kwast Kurt E |
| USA | Rochester | Nair K Sreekumaran |
| USA | Davis | Adams Sean H |
| USA | Bethesda | Fessel Joshua Patrick |
| USA | Provo | Hancock Chad R |
| USA | Pittsburgh | Prochownik Edward V |
| USA | Galveston | Durham William J |
| USA | Louisville | Skolik Robert A |
| USA | Gainesville | Leeuwenburgh Christiaan |
| USA | Aurora | Ford Ellen |
| USA | Ann Arbor | Tyrrell Daniel J |
| USA | San Diego | Cannon Daniel T |
| USA | Milwaukee | Dash Ranjan K |
| USA | Seattle | Marcinek DJ |
| USA | Fort Collins | Li Puma Lance C |
| USA | Philadelphia | Towheed Atif |
| USA | Wakefield | Varricchio Frederick |
| USA | Norfolk | Lai Nicola |
| USA | Omaha | Kim Julian KS |
| USA | Bethesda | Sharma Pushpa |
| USA | Denton | Dzialowski Edward M |
| USA | Portland | Garlid Keith D |
| USA | Orlando | Coen Paul M |
| USA | Winston-Salem | Tomar Dhanendra |
| USA | Rochester | Lanza Ian R |
| USA | East Lansing | Bazil Jason N |
| USA | Chicago | Dayanidhi Sudarshan |
| USA | Denver | Van Hove Johan |
| USA | Kansas City | Swerdlow Russell H |
| USA | Orlando | Goodpaster Bret H |
| USA | Colorado Springs | Jacobs RA |
| USA | Corvallis | Batterson Philip M |
| USA | Stanford | Stary Creed |
| USA | Durham | Li Pingan Andy |
| USA | Aurora | Sparagna Genevieve C |
| USA | El Paso | Bajpeyi Sudip |
| USA | Fayetteville | Iyer Shilpa |
| USA | Fairbanks | Coker Robert H |
| USA | Baton Rouge | Irving Brian A |
| USA | Corvallis | Robinson Matthew M |
| USA | Little Rock | MacMillan-Crow Lee Ann |
| USA | Gainesville | Neyroud D |
| USA | Orlando | Pino Maria F |
| USA | Fayetteville | Rao RR |
| USA | Baton Rouge | Hand Steven C |
| USA | Little Rock | Borsheim Elisabet |
| ... further results | ||
Task groups
- TG leaders: suggestions discussed at the MitoFit Science Camp 2016 Kuehtai AT.
- TG participants: listed as a summary from the feedback received.
TG1.1 Coupling states and coupling control ratios: Consensus statements
- TG leaders: Renner-Sattler Kathrin DE / Gnaiger Erich AT / Hoppel Charles L US
- TG participants:
TG1.2 SUIT protocols: nomenclature consistent with concept of database
- TG leader: Doerrier Velasco Carolina A AT, Gnaiger Erich AT
- TG participants: Attimonelli Marcella IT, Krako Nina RS
TG1.3 Experimental details in SUIT
- TG leaders: Chicco Adam J US/ Hickey Anthony J NZ/ Doerrier Velasco Carolina A AT
- TG participants: Casado Pinna Marta ES, Krako Nina RS
TG1.4 Instrumental platforms: comparisons
- TG leaders: Elmer Eskil SE (suggested), Molina Anthony JA US
- TG participants: Canto Carles CH (suggested), Genova Maria Luisa IT, Tronstad Karl Johan NO (suggested), Zorzano Antonio ES (suggested)
TG1.5 Knowledge management system
- TG leaders: Drinnan Michael UK/ Plattner Christina AT
- TG participants:
TG1.6 Proficiency test
- TG leaders: Gnaiger Erich AT/ Doerrier Velasco Carolina A AT
- TG participants: Genova Maria Luisa IT, Plattner Christina AT
TG1.7 Documentation
- TG leaders: Arnould Thierry BE/ Votion Dominique-Marie BE
- TG participants:
TG1.8 Translation to community in general
- TG leader: Irving Brian A US
- TG participants: Krako Nina RS
TG1.9 Dissemination Scientific community (formally WG5)
- TG leaders: Makrecka-Kuka Marina LV / Gnaiger Erich AT
- TG participants: Ali Sameh S EG, Cervinkova Zuzana CZ, Chicco Adam J US, Coen Paul M US, Eide Lars NO, Garcia-Roves Pablo M ES, Gasparre Giuseppe IT, Genova Maria Luisa IT, Haendeler Judith DE, Iyer Shilpa US, Krako Nina RS, Mayan Maria D ES, Mishmar Dan IL, Nehlin Jan DK, Palmeira Carlos ES, Paterlini Serena IT, Porcelli Anna Maria IT, Rao Raj US, Schaefer Patrick DE, Tronstad Karl Johan NO, Trougakos Ioannis GR, Volani Chiara AT, Votion Dominique-Marie BE
TG1.10 Training, Short Term Scientific Missions (formally WG5)
- TG leader: Klaus Susanne DE (suggested), Labieniec-Watala Magdalena PL (suggested)
- TG participants: Ali Sameh S EG, Cervinkova Zuzana CZ, Chicco Adam J US, Coen Paul M US, Eide Lars NO, Garcia-Roves Pablo M ES, Gasparre Giuseppe IT, Genova Maria Luisa IT, Haendeler Judith DE, Iyer Shilpa US, Krako Nina RS, Mayan Maria D ES, Mishmar Dan IL, Nehlin Jan DK, Palmeira Carlos ES, Paterlini Serena IT, Porcelli Anna Maria IT, Rao Raj US, Schaefer Patrick DE, Tronstad Karl Johan NO, Trougakos Ioannis GR, Volani Chiara AT, Votion Dominique-Marie BE
Comments and discussion
- We are mostly interested in two WG: (1) Since we are working mostly with heart and muscles, we are interested in WG 2 - MITOEAGLE data repository in muscle and other tissues. (2) WG 1 - Standard operating procedures and user requirement document: Protocols, terminology, documentation. Since we have an expertise in fatty acid metabolism, we are specially interested in experimental protocols for the evaluation of mitochondrial capacities regarding fatty acid metabolism. Of course our parts of the WG are also in our interest. - Marina Makrecka-Kuka, Edgars Liepinsh (2016).
- My role in Mito-EAGLE would be mainly in WG 2 and 4, with a focus on skeletal and cardiac muscle and cultured cardiomyocytes and endothelial cells. I would particularly like to bridge the gap between the group leaders and young investigators, trying to represent the young people who are the active users of the OROBOROS equipment. This will fit in nicely with the goals in WG1. ... Major congratulations on obtaining this beautiful collaborative grant! Very glad to see this network getting the recognition it deserves! Looking forward to being part of this! - Rob C Wuest (2016).
- Please, find enclosed my declaration to participate with my team in the following workgroups: WG1, WG2, WG3, WG4. We are now involved and have been in not a distant past – in studying of the following cell /tissue types:
- Blood cells, with particular emphasis on platelets and lymphocytes – isolated from animals and humans.
- Endothelial cells, fibroblasts, neurocytes (neuroblastoma), astrocytes, cancer cell lines – predominantly cultured.
- Hepatocytes, cardiomyocytes, cell isolated from brain – isolated from lab animals. - Cezary Watala (2016).
- I have already contacted the portuguese CNC to nominate me for the MC of theaction. Concerning my active participation, taking into account the type of work we do in my lab I could be included in all WGs. As you know we work with mitochondria from liver, muscle and fat, as well as cultured cells from different origins (WG2-4). The elaboration of SOPs (WG1) we can certainly contribute (see the book I edited on Mitochondrial Bioenergetics and in which you contribute a chapter). I believe that everybody can be part of the WG5, but as the coordinators of the action, you and Sandra should decide what is best for the success of the action. - Carlos Palmeira (2016).
- I would propose my participation in the WG1: Standard operating procedures and user requirement document: Protocols, terminology, documentation, considering that the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. I could give my active contribution in the phase of making platform that will aggregate the Action’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases in particular tasks:
- Develop reccomendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalised service for data management, data mining and health-care conforming standards of data interpretation
- Provide a summary on strategic dissemination and an education programme for MITOEAGLE.
- Considering that we are in process of buying the oxygraph, thus broading capacities of my lab, I would be happy to open the door of my new lab for the innovative inter-laboratory ring test experiments and together with my team contribute in that phase of the MITOEAGLE Action. I look forward to the establishment of MitoGlobal EAGLE network where, as stated in the MoU, researchers will collaborate on mapping mitochondrial physiology and medicine. The creation of such a unique well-coordinated network of senior researchers and young investigators will definitely put an added value to existing data and publications, will increase quality and validity of the research, will bring new possibilities of data sharing and data mining and will hopefully bring us to the era of more economical research by saving resources through standardised and harmnonised protocols, where the measuremnts will not be wasted due to lack of comparability. - Nebojsa Lalic (2016).
- Our research interest is oriented to reproductive endocrinology; especially to mechanisms that govern steroidogenesis of testicular Leydig cells in disturbed homeostasis. We use several in vivo models:
- stress -/+ systemic or intratesticular blockade of different stress receptors (alpha1ADR; betaADR; GR)
- hypogonadal-hypogonadism -/+ testosterone-replacement therapy
- androgens anabolics treatment -/+ blockade od AR
- aged rats -/+ Viagra-treatment
- biological clock - Silvana Andric (2016).
- I took a look to the MoU and the different working groups. You know well my research focus and having this into account I am interested in actions related to Working group 1 (Respirometric reference protocols, Interlaboratory proficiency test, Instrumental platforms). Working group 2 and 3: I have generated multiple mitochondrial respiration data sets in different mouse tissues (mainly in skeletal muscle, hypothalamus, liver and white adipose tissue). Currently we are also performing these assays in white adipose tissue from human patients. Hence I am interested in all milestones of WG 2 and 3. WG 5 is related to WG 1 and as you know I have interest in training, dissemination and also together with my collaborators (if needed) we could help with data sets and a data interpretation. Overall, I have a general interest on this Action and my active role in MITOEAGLE could be discussed. I could be of help participating in some of the tasks where my expertise and interest fit best. We will be forming and interdisciplinary research group with four group leaders: JC Perales, R Guimera, M Sales-Pardo and PM Garcia-Roves with two different affiliations, University of Barcelona (JCP and PMG-R - Energy metabolism) and University Rovira I Virgili (RG and MS – Data base, statistics, data mining). - Pablo M Garcia-Roves (2016).
- I will be pleased to contribute to the tasks of WG1 by developing interlaboratory proficiency tests and instrumental platform comparison. Moreover, as a (potential) MC member of MitoEAGLE I can also offer visibility to the Action itself (i.e. WG5: MITOEAGLE dissemination and training, according to mitoglobal.org webpages) by establishing new interactions with the International Coenzyme Q10 Association (ICQA) where I act as a member of the Executive Committee. ICQA organizes biennal conferences on new highlights of Coenzyme Q research. In 2015, we held the conference in Bologna (http://conference2015.wix.com/icqaconference) and we included in the program also some scientific sessions dedicated to mitochondrial bioenergetics and diseases. I will be pleased to enforce cooperation between ICQA and MitoEAGLE. - Maria Luisa Genova (2016).
- We found the scientific content and objectives of the call very interesting and compatible with our wide expertise on oxysterol analysis in food and biological systems. Our group has a large experience on lipid composition and their degradation products (volatile and non-volatile), as well as on sensory analysis. Moreover, we are currently involved in the H2020-GERI-2014-1 project "PLOTINA" ("Promoting gender balance and inclusion in research, innovation and training") with Prof. Gallina Toschi (in cc) as coordinator; the project actually includes gendered case studies where gender and sex variables are considered. You can find further information about our expertises, publications and the PLOTINA project at the following webpages: https://www.unibo.it/sitoweb/maria.rodriguez/, https://www.unibo.it/sitoweb/tullia.gallinatoschi/, http://www.unibo.it/en/research/projects-and-initiatives/research-projects-horizon-2020-1/plotina - Maria Teresa Rodriguez Estrada (2016).
- As you know, our current/developing interests are with "evolution" (species comparisons) and "environment" (hypoxia and cold exposure), but have also experience and interest in assessing effects of "lifestyle" (diet, exercise) and "aging" as well (in rodents). - Adam J Chicco (2016).
- Congratulations on the considerable progress with MITOEAGLE! I am keen to participate in this exciting project and I’m sure I can make a valuable contribution to it's success. I have extensive experience performing respirometry assays in human muscle biopsies, myoblasts/tubes and mouse tissues in the contexts of aging, obesity, exercise and inactivity. I feel that I could contribute to any of the five working groups, but particularly to WG2, WG4, and WG5. I am also happy and willing to serve on the Management Committee if needed. Again, congratulations on organizing such an exciting project. - Paul M Coen (2016).
- I would be interested to be involved with WG1 and WG2. In particular, I am interested in contributing to developing protocols for multicenter quality control among labs, and for permeabilized muscle bundle respirometry. - Harry B Rossiter (2016).
Projects in progress
- Interlaboratory proficiency ring test
- Testing of software components
- Concepts and terminology on mitochondrial physiology
- Procedures and experimental protocols for the evaluation of mitochondrial capacities, library of protocols
- Data management system (DMS)
- Database
Next steps
- Best practices for implementation of analytical and process standards.
References
- General requirements for the competence of testing and calibration laboratories » ISO/IEC 17025:2005
