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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE protocols, terminology, documentation
WG1
- Standard operating procedures and user requirement document: Protocols, terminology, documentation
WG1 Project application
Description
- The quality management system, scope of SOPs to be elaborated and the outline of the data repositories will be defined in a user requirement document.
Respirometric reference protocols
- A ‘library of protocols’ applied in mitochondrial respiratory physiology will be collected in a standard format, delineating the diversity of experimental approaches to assess bioenergetic function. A set of reference protocols will rigorously document (Maelstrom Research program) the state-of-the-art standards in designing, conducting, reporting, interpreting, and validating such bioenergetic tests (see also figure on the right).
Interlaboratory proficiency test
- Inter-laboratory ring tests are a basic requirement for quality management. Such proficiency tests require a reference sample which must be homogenous, stable, representative of the diagnostic target, amenable for distribution, and economical for large-scale production. For respiratory OXPHOS analysis a reference sample of structurally and functionally intact mitochondria is not yet available to perform corresponding ring tests (in contrast to enzymatic OXPHOS ring tests). Recently, the widely applied human cell line HEK 293T was found to be potentially suitable for cryopreservation. Upon thawing the cells can be used immediately for respirometry of both intact and permeabilized cells. Thus interlaboratory proficiency testing may now be feasible as a world-wide innovation in the field of mitochondrial respiratory physiology. Participating labs may implement the test for intra- and interlaboratory validation and longitudinal performance monitoring, using a reference OXPHOS protocol (Fig. 3). The organization of the proficiency test should follow the requirements of the ISO 17043 and 13528 standards. A corresponding MITOEAGLE proficiency training shall be developed and implemented. Feedback from the participants will lead to a final adjustment of the SOPs in the proficiency test, which will be made generally available.
Instrumental platforms
- Comparison of results between and within instrumental platforms: The reference protocols and proficiency test will not define the instrumental platform, but will allow a quantitative comparison of results obtained with specific equipment available in the participating laboratories.
Nomenclature
- Harmonization of nomenclature on mitochondrial respiratory states and control parameters: The logistics of development of a database requires application of strictly defined terms for all included variables. There is no general reference available upon which a consistent terminology on mitochondrial physiology and bioenergetics can be based. The MITOEAGLE consortium, therefore, has to accomplish the ambitious goal to unify and simplify the terminology in the field for the purpose of the QMS, which will lead to the development of recommendations for the use of a common terminology in mitochondrial physiological research. A publication will be prepared as an Open Access article which will be a milestone towards a unification of concepts and nomenclature.
Knowledge management system
- With an increase in data volume and complexity comes along the requirement for standardized report formats and digital accessibility of the data. Since such a database is still missing for mitochondrial physiology, the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. Databases will be established combining biological and clinical data comprising proprietary and publicly available datasets into one integrated solution. Data import and export functionalities will allow data upload and mapping to corresponding objects in the database, in this way generating valuable data and allowing for project-specific as well as cross-project data mining.
- The ability to formulate meta-studies in our approach is an important success factor: The platform can aggregate the project’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases. In addition to functional data, other data types such as nutritional or environmental factors associated with disease prevention can be identified and aggregated into the MITOEAGLE Knowledge Management System.
- Querying the heterogeneous data types and result representation are the most important requirements of the knowledge platform besides to data integration and modelling. The application will feature different types of visualization techniques for all integrated data types. The database will be developed using the concept of multi-tier client-server architecture. Individual components are separated corresponding to their functionality. An upstream web server handles user requests, an application server performing business logic, and a back-end database system guaranties persistent data storage. All components are additionally separated by means of network security incorporating firewalls and are hosted in a secure environment. The platform administration ensures privacy regarding proprietary data by user rights policies.
- It will be the task of WG1 to continuously collect the input from all other WGs, compare, discuss and align SOPs as far as possible, and to finally develop recommendations for quality control, data reporting and data sharing beyond the published record. In addition, WG1 will develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation. Finally, it will provide a summary on strategic dissemination and an education programme for MITOEAGLE. The education programme will ensure that not only presently participating groups will adhere to the set standards but that also groups joining in at later points may smoothly fit into the community and help to further broaden and deepen our understanding on mitochondrial physiology in health and disease.
Management
Tasks
- Opening of the database to the research public for data search and moderated entry of new datasets.
- Development of interlaboratory proficiency ring test, of recommendation/certification reports and of a MITOEAGLE proficiency training module.
- Sample distribution, ring test experiments (MITOEAGLE-proficiency training on two different points of time, 6 months apart), data analysis by experimenters and MITOEAGLE-proficiency test manager.
- Reports and publication: Reports with specific results in comparison with an anonymous data summary, and joint publication. Individual reports may be complemented by recommendations, and are summarized in an informal certification report.
- Instrumental platform comparison with reference sample applying comparable measurements.
- Draft, dissemination, collection and final formulation of a manuscript on concepts and terminology of mitochondrial physiology.
- Collect and discuss procedures and experimental protocols for the evaluation of mitochondrial capacities and create a library of protocols: A database comprising reference SUIT protocols, standard experimental media, and detailed instructions for OXPHOS analysis is assembled.
- MITOEAGLE data management system (DMS) development: The DMS for further data preprocessing. The DMS will enable management and analysis of instrument-specific and project-specific data.
- MITOEAGLE database (DB) development: DB will facilitate high-level project specific analysis as well as meta-analyses across projects, visualization of project specific data as well as integrative analyses of datasets for various proprietary and/or publicly available datasets.
- Testing of developed software components regarding functionality and usability, modification and implementation. Deployment for routine testing in the labs of our strategic partners.
- Data input: Upload of datasets generated through WG 2-4 in internal lab developments, results of the proficiency ring test, and during application module developments. In addition, the database will be populated with available proprietary and public datasets.
- Develop recommendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation.
- Provide a summary on strategic dissemination and an education programme for MITOEAGLE.
Milestones
- Data sets entered in standardized format and database opened for the public
- Protocols for ring-tests and standard analysis and report scheme defined
- MITOEAGLE proficiency training workshop finished
- Analysis is completed and joint publication submitted
- Publication of paper on concepts and terminology
- Library of protocols online
- Publication of recommended protocols and procedures
- Guidelines for acquisition, evaluation and data documentation available
- MITOEAGLE data management system finished
- MITOEAGLE database ready for use
- Software modifications incorporated following extensive tests
- Publication of MITOEAGLE-KMP
- Recommendations on data acquisition, evaluation and management published
- Concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation published
Deliverables
- Guidelines for future research and recommendations for the evaluation of respiratory characteristics in human respirometric samples
- A database on mt fitness evaluated with human cells and tissues
- Training of researchers towards improved reproducibility of sample preparation and respirometric evaluation
- Qualitative and quantitative evaluation of results obtained in ongoing studies by comparison with a reference sample
- An expanded MITOEAGLE database
- A joint publication documenting the results of the MITOEAGLE-PT
- A multi-authored publication towards a unification of concepts and nomenclature in mitochondrial physiology
- A library of protocols for sample preparation and examination by respirometry
- A multi-authored publication with recommendations of comparable standard protocols and procedures.
- Guidelines as to the optimum use of a reference sample for determination of respiration and related parameters of interest.
- The MITOEAGLE data management system.
- A continuously updated MITOEAGLE database for the use as reference data and guideline towards application of optimized protocols, ultimately leading towards standardization.
- A joint publication on the use of the MITOEAGLE database and management system.
- An education programme for the use of MITOEAGLE database and management system.
WG1 Action
- Many analyses lack statistical power within a single study. Statistical combination of different studies is often difficult due to lack of methodological standardization and harmonization.
WG Leader
- Kathrin Renner-Sattler, Mag. Dr. - [email protected]
WG Co-Leader
- Marko Vendelin, Dr. - [email protected]
Participants
| Country | City | Contact |
|---|---|---|
| Armenia | Yerevan | Trchounian K |
| Australia | Queensland | Dong Lan-Feng |
| Australia | Sydney | Krycer James R |
| Australia | Queensland | Fisher Joshua J |
| Australia | Parkville | Hardee Justin P |
| Australia | Queensland | Holland Olivia J |
| Australia | Victoria | Van Bergen Nicole J |
| Australia | Melbourne | Ferri Alessandra |
| Australia | Melbourne | Saner Nicholas |
| Australia | Melobourne | Botella Javier |
| Australia | Southport | Vidimce Josif |
| Australia | Melbourne | Kuang Jujiao |
| Australia | Melbourne | Whitfield Jamie |
| Australia | Nedlands | Filipovska Aleksandra |
| Australia | Melbourne, VIC | Muccini Anna Maria |
| Australia | Queensland | Radenkovic Filip |
| Australia | Clayton | Wolff Jonci Nikolai |
| Australia | Syndey | Ton Riccardo |
| Australia | Victoria | Winwood-Smith Hugh |
| Australia | Melbourne | Hewakapuge Sudinna |
| Australia | Nedlands | Perks Kara L |
| Australia | Melbourne | Lionett S |
| Australia | Clayton Victoria | Alton Lesley |
| Australia | Melbourne | Coughlan Melinda T |
| Australia | Newtown, Sydney | Stocker Roland |
| Australia | Sydney | Hoehn Kyle L |
| Australia | Geelong, Victoria | McKenzie Matthew |
| Australia | Melbourne | Cassar Samantha |
| Australia | Melbourne | Stepto Nigel K |
| Australia | Burwood | Trewin Adam J |
| Australia | Melbourne | Genders Amanda J |
| Austria | Innsbruck | Keller Markus A |
| Austria | Innsbruck | Passrugger Manuela |
| Austria | Innsbruck | Klocker Helmut |
| Austria | Salzburg | Kofler B |
| Austria | Innsbruck | Plangger Mario |
| Austria | Vienna | Kozlov Andrey V |
| Austria | Innsbruck | Thapa Maheshwor |
| Austria | Innsbruck | Karabatsiakis Alexander |
| Austria | Innsbruck | Schmitt Sabine |
| Austria | Innsbruck | Schartner Melanie |
| Austria | Innsbruck | Burtscher Martin |
| Austria | Innsbruck | Nindl Veronika |
| Austria | Innsbruck | Cecatto Cristiane |
| Austria | Innsbruck | Bergmeister Lisa |
| Austria | Innsbruck | Di Marcello Marco |
| Austria | Innsbruck | Antunes Diana |
| Austria | Innsbruck | Hoppel Florian |
| Austria | Kapferer Werner | |
| Austria | Innsbruck | Doerrier Carolina |
| Austria | Innsbruck | Gallee Leon |
| Austria | Innsbruck | Cardoso Luiza HD |
| Austria | Innsbruck | Perez Valencia Juan Alberto |
| Austria | Innsbruck | Schwarzer Christoph |
| Austria | Aldrans | Haider Markus |
| Austria | Innsbruck | Sobotka Ondrej |
| Austria | Klosterneuburg | Sazanov Leonid A |
| Austria | Innsbruck | Garcia-Souza Luiz F |
| Austria | Innsbruck | Meszaros Andras T |
| Austria | Innsbruck | Laner Verena |
| Austria | Innsbruck | Goebel G |
| Austria | Innsbruck | Gnaiger Erich |
| Belgium | Brussels | Sonveaux P |
| Belgium | Namur | Arnould Thierry |
| Belgium | Liege | Votion Dominique-Marie |
| Belgium | Leuven, Flanders | Baekelandt V |
| Belguim | Sart Tilman (Liège) | Kruse C |
| Brasil | Porto Alegre | Haas Clarissa |
| Brasilia | Limeira | Ropelle Eduardo R |
| Brazil | São Paulo | Crispim Marcell |
| Brazil | São Paulo | Kowaltowski Alicia J |
| Brazil | Santa Maria - RS | Goncalves Debora Farina |
| Brazil | Jaboticabal | Oliveira Marcos Tulio |
| Brazil | Sao Paulo | Silber Ariel Mariano |
| Brazil | Santa Maria - Rio Grande do Sul | Soares Felix Alexandre Antunes |
| Brazil | Rio de Janeiro | Galina Antonio |
| Brazil | Rio de Janeiro | Oliveira Marcus F |
| Brazil | Sao Paulo | Vercesi Anibal E |
| Brazil | Rio de Janeiro | Vieyra Adalberto |
| Canada | Winnipeg | Chowdhury Subir Roy |
| Canada | Guelph | Holloway Graham P |
| Canada | Ottawa | Darveau Charles-A |
| Canada | Edmonton | Han Woo Hyun |
| Canada | Vancouver, BC | Tausan Daniel |
| Canada | Quebec | Joseph Vincent |
| Canada | Charlottetown | Adiele Reginald C |
| Canada | Moncton, NB | Pichaud Nicolas |
| Canada | Rimouski | Blier Pierre U |
| Canada | Edmonton (Alberta) | Lemieux Helene |
| Canada | Edmonton | Zaugg Michael |
| Canada | Montréal | Breton Sophie |
| Canada | Antigonish | Weir M |
| Canada | Hamilton | Scott Graham R |
| Canada | Rimouski | Munro Daniel |
| Canada | Ottawa | Harper Mary-Ellen |
| Canada | Montreal, Quebec | Bergdahl Andreas |
| Canada | Vancouver | Bovard Josh |
| Canada | Edmonton | Lucchinetti Eliana |
| Canada | Saint John | Pulinilkunnil Thomas |
| Canada | Hamilton | Tarnopolsky Mark A |
| Chile | Coyhaique | Jana Prado Fabian |
| China | Xi`an | Liu Jiankang |
| China | Pukou, Nanjing, | Gan Zhenji |
| China | Tianjin | Zhang Yong |
| China | Fuzhou, Fujian Province | Chen Qi |
| Croatia | Zagreb | Rubelj Ivica |
| Croatia | Zagreb | Saric A |
| Croatia | Zagreb | Curik I |
| Croatia | Split | Ljubkovic M |
| Czech Republic | Pardubice | Rousar Tomas |
| Czech Republic | Plzen -Severni predmesti | Jedlicka Jan |
| Czech Republic | Prague | Drahota Zdenek |
| Czech Republic | Prague | Krizova J |
| Czech Republic | Vestec | Rohlena Jakub |
| Czech Republic | Pardubice | Majtnerova Pavlina |
| Czech Republic | Prague | Houstek Josef |
| Czech Republic | Ceske Budejovice | Zikova Alena |
| Czech Republic | Pardubice | Handl Jiri |
| Czech Republic | Hradec Kralove | Endlicher Rene |
| Czech Republic | Praha 2 | Danhelovska Tereza |
| Czech Republic | Vestec | Ezrova Zuzana |
| Czech Republic | Hradec Kralove | Kucera Otto |
| Czech Republic | Praha 2 | Zdrazilova Lucie |
| Czech Republic | Vestec | Davidova Eliska |
| Czech Republic | Prague 10 | Urban Tomas |
| Czech Republic | Hradec Kralove | Stankova Pavla |
| Czech Republic | Hradec Kralove | Cervinkova Zuzana |
| Czech rep | Hradec Kralove | Falaye T |
| Denmark | Copenhagen | Larsen Steen |
| Denmark | Aarhus N | Jespersen Nichlas Riise |
| Denmark | Copenhagen | Gonzalez-Franquesa Alba |
| Denmark | Copenhagen | Kraunsoee R |
| Denmark | Copenhagen | Soendergaard SD |
| Denmark | Hvidovre | Nehlin Jan O |
| Denmark | Copenhagen N | Mortensen OH |
| Denmark | Roskilde | Dalgaard Louise T |
| Denmark | Copenhagen N | Maise Chroeis K |
| Denmark | Copenhagen | Dela Flemming |
| Denmark | Aarhus N | Boetker Hans Erik |
| Denmark | Copenhagen N | Scheibye-Knudsen Morten |
| Denmark | Copenhagen | Nielsen B |
| Egypt | Cairo | Ali Sameh S |
| Egypt | Cairo | Abdel-Rahman Engy Ali |
| Estonia | Tallinn | Klepinin Aleksandr |
| Estonia | Tallinn | Puurand Marju |
| Estonia | Tallinn | Shevchuk Igor |
| Estonia | Tallinn | Vendelin Marko |
| Estonia | Tallinn | Laasmaa Martin |
| Estonia | Tartu | Paju Kalju |
| Estonia | Tallinn | Karro N |
| Estonia | Tallinn | Truu Laura |
| Estonia | Tallinn | Kaambre Tuuli |
| Estonia | Tallinn | Klepinina Lyudmila |
| Estonia | Tallinn | Tepp Kersti |
| Finland | Turku | Stier Antoine |
| Finland | Helsinki | Jackson Christopher Benjamin |
| Finland | Jyvaeskylae | Kainulainen Heikki |
| Finland | Helsinki | Suomalainen Wartiovaara Anu |
| France | Angers | Procaccio Vincent |
| France | Bordeaux | Amoedo Nivea Dias |
| France | Gueguen Naig | |
| France | Bordeaux | Sarlak Saharnaz |
| France | Maisons-Alfort Cedex | Prola Alexandre |
| France | Bordeaux cedex | Mourier A |
| France | Tours | Dumas Jean-Francois |
| France | Plouzané | Salin Karine |
| France | Bordeaux cedex | Rossignol Rodrigue |
| France | Paris | Armand Anne-Sophie |
| France | Angers | Spinazzi Marco |
| France | Martinique | Neviere Remi |
| France | Montpellier | Wrutniak-Cabello Chantal |
| France | Nouzilly | Collin-Chenot Anne |
| France | Paris | Bouillaud Frederic |
| France | Lille | Montaigne David |
| France | Martinique | Cano Sanchez Maria Consolacion |
| Germany | Regensburg | Renner-Sattler Kathrin |
| Germany | Munich | Zischka Hans |
| Germany | Gießen | Grewal Rekha |
| Germany | Magdeburg | Schoenfeld Peter |
| Germany | Marburg | Vogt Sebastian |
| Germany | Cologne | Ho Dieu Hien |
| Germany | Giessen | Schulz Rainer |
| Germany | Cologne | Wiesner Rudolf J |
| Germany | Ulm | Schaefer PM |
| Germany | Hannover | Das Anibh Martin |
| Germany | Jena | Szibor Marten |
| Germany | Wilhelmshaven | Salmon Pablo |
| Germany | Cologne | Maciej Sarah |
| Germany | Cologne | Trifunovic Aleksandra |
| Germany | Marburg | Ramzan Rabia |
| Germany | Düsseldorf | Goy C |
| Germany | Freiburg | Schuele R |
| Germany | Rostock | Sokolova Inna |
| Germany | Duesseldorf | Roden Michael |
| Germany | Duesseldorf | Haendeler Judith |
| Germany | Frankfurt | Osiewacz Heinz D |
| Germany | Frankfurt | Hamann Andrea |
| Germany | Giessen | Sommer Natascha |
| Germany | Wickert Anika | |
| Germany | Neuherberg | Einer C |
| Germany | Biberach an der Riß | Nold V |
| Germany | Gießen | Silaidos Carmina |
| Germany | Frankfurt | Warnsmann Verena |
| Germany | Duesseldorf | Jelenik T |
| Germany | Tuebingen | Kappler Lisa |
| Germany | Doermann Niklas | |
| Germany | Frankfurt am Main | Viel Christian |
| Germany | Cologne | Pesta Dominik |
| Germany | Mainz | Methner Axel |
| Germany | Bremerhaven | Mark Felix Christopher |
| Germany | Mainz | Maull Felicia |
| Germany | Duesseldorf | Piel Sarah |
| Greece | 15771 | Andreadou Ioanna |
| Greece | Athens | Trougakos Ioannis P |
| Greece | Heraklion | Tavernarakis Nektarios |
| Greece | Thessaloniki | Lazou Antigone |
| Greece | Athens | Gumeni Sentiljana |
| Hungary | Budapest | Tretter Laszlo |
| Hungary | Debrecen | Verebne Tar K |
| Hungary | Budapest | Komlodi Timea |
| Hungary | Szeged | Ferdinandy Peter |
| Hungary | Szeged | Boros Mihaly |
| Hungary | Budapest | Horvath G |
| Hungary | Budapest | Chinopoulos Christos |
| India | New Delhi | Vincent Vinnyfred |
| India | Kollam | Suravajhala Prashanth |
| India | Haryana | Chakrabarti Sasanka |
| India | Lucknow | Gayen Jiaur |
| India | Varanasi | Sonkar Vijay K |
| India | Hyderabad | Jha Rajan Kumar |
| India | New Delhi | Thakkar Himani |
| Iran | Tehran | Shirazi Reza |
| Iran | Tehran | Safaei Zahra |
| Ireland | Dublin | O'Gorman Donal |
| Ireland | Dublin | Porter Richard K |
| Israel | Beer Sheva | Mishmar Dan |
| Israel | Rishon Le Zion | Hachmo Yafit |
| Israel | Zrifin | Sova Marina |
| Israel | Jerusalem | Saada Reisch Ann |
| Italia | Udine | Salvadego Desy |
| Italien | Padova PD | Szabo Ildiko |
| Italy | Bologna | Porcelli AM |
| Italy | Milan | Clementi Emilio |
| Italy | Catania | Messina Angela |
| Italy | Ancona | Battino Maurizio |
| Italy | Catania | De Pinto Vito |
| Italy | Bologna | Gasparre G |
| Italy | Padova | Fernandez-Vizarra Erika |
| Italy | Bolzano/Bozen | Volani Chiara |
| Italy | Padova | Viscomi Carlo |
| Italy | Bari | Attimonelli M |
| Italy | Bolzano | Zanon Alessandra |
| Italy | Padova | Bernardi Paolo |
| Italy | St. Lorenzen | Harrison David K |
| Italy | Bologna | Genova Maria Luisa |
| Italy | Campobasso | Pallotta Maria Luigia |
| Italy | Udine | Grassi B |
| Italy | Rome | Scatena Roberto |
| Italy | Monserrato | Isola Raffaella |
| Italy | Bologna | Paterlini S |
| Italy | Milano | Bottoni P |
| Italy | Catania | Magri Andrea |
| Japan | Sapporo | Matsumoto J |
| Japan | Itabashi-ku Tokyo | Tanaka Masashi |
| Kitzbühel | Innsbruck | Fischer Michael J |
| Korea | Seoul | Pak Youngmi Kim |
| Korea | Incheon | Kwak Hyo Bum |
| Korea (South) | Seoul | Lee Hong Kyu |
| Latvia | Riga | Dambrova Maija |
| Latvia | Riga | Vilks Karlis |
| Latvia | Riga | Liepins Edgars |
| Latvia | Riga | Svalbe Baiba |
| Latvia | Riga | Volska Kristine |
| Latvia | Riga | Zvejniece Liga |
| Latvia | Riga | Stelfa Gundega |
| Latvia | Riga | Inashkina I |
| Latvia | Riga | Jansone Baiba |
| Luxembourg | Strassen | Devaux Yvan |
| Malaysia | Kuala Lumpur | Hassan Hazirah |
| Malta | Msida | Vella Joanna |
| Mexico | Tlalpan | Rodriguez-Enriquez Sara |
| Mexico | Mexico City | Aparicio Trejo OE |
| México | San Pedro Garza García, Nuevo Leon | Garcia-Rivas Gerardo |
| Netherlands | GA Wageningen | Grefte Sander |
| Netherlands | Maastricht | Grevendonk L |
| New Zealand | Wellington | Berridge Michael V |
| New Zealand | Auckland | Kaur Sarbjot |
| New Zealand | Auckland | Hickey Anthony J |
| New Zealand | Auckland | Ward Marie Louise |
| Norway | Bergen | Pettersen Nitschke Ina Katrine |
| Norway | Trondheim | Grill V |
| Norway | Trondheim | Lerfall Joergen |
| Norway | Bergen | Berge Rolf K |
| Norway | Bergen | Tronstad Karl Johan |
| Norway | Bergen | Haavik Jan |
| Norway | Trondheim | Smenes Benedikte Therese |
| Norway | Oslo | Eide L |
| Norway | Trondheim | Rognmo O |
| Norway | Bergen | Hoel Fredrik |
| Norway | Bergen | Dyrstad Sissel E |
| Norway | Tromsoe | Boardman Neoma T |
| Norway | Bergen | Blindheim Dan Filip |
| Norway | Bergen | Roesland Gro Vatne |
| Poland | Poznan | Jarmuszkiewicz Wieslawa |
| Poland | Gdańsk | Kaczor Jan Jacek |
| Poland | Warsaw | Wieckowski Mariusz R |
| Poland | Warsaw | Zablocki Krzysztof |
| Poland | Lodz | Watala Cezary |
| Poland | Warsaw | Sek Aleksandra |
| Poland | Warsaw | Karkucinska-Wieckowska Agnieszka |
| Poland | Warsaw | Dymkowska Dorota |
| Poland | Warsaw | Wasilewski M |
| Poland | Lodz | Labieniec-Watala Magdalena |
| Poland | Krakow | Dembinska-Kiec Aldona |
| Poland | Warsaw | Szewczyk Adam |
| Poland | Warsaw | Kampa Rafal Pawel |
| Poland | Warszawa | Jaskiewicz Anna |
| Poland | Warsaw | Bednarczyk Piotr |
| Portugal | Cantanhede | Rodrigues Ana Sofia |
| Portugal | Porto | Crisostomo Luis |
| Portugal | Porto | Moreira Bruno P |
| Portugal | Coimbra | Rolo Anabela Pinto |
| Portugal | Porto | Silva Ana Maria |
| Portugal | Cantanhede | Amorim Ricardo |
| Portugal | Coimbra | Dias Candida |
| Portugal | Porto | Beleza Jorge |
| Portugal | Coimbra | Duarte Filipe Valente |
| Portugal | Porto | Alves Marco G |
| Portugal | Coimbra | Palmeira Carlos |
| Portugal | Cantanhede | Teodoro Joao Soeiro |
| Portugal | Coimbra | Grilo Luis |
| Portugal | Coimbra | Oliveira Paulo J |
| Portugal | Coimbra | Minuzzi Luciele M |
| Portugal | Coimbra | Santos Diana |
| Portugal | Lisboa | Macedo Maria Paula |
| Portugal | Aveiro | Ferreira Rita Maria P |
| Portugal | Porto | Oliveira Jorge |
| Portugal | Aveiro | Vitorino Rui Miguel Pinheiro |
| Portugal | Porto | Oliveira Pedro Fontes |
| Qatar | Doha | Guarch Meritxell Espino |
| Republic of Ghana | Cape Coast | Tei BN |
| Republic of Korea | Daejeon | Park SH |
| Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
| Republic of Korea | Busan | Kim Hyoung Kyu |
| Republic of Korea | Busan | Han Jin |
| Republic of Serbia | Novi Sad | Andric Silvana |
| Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
| Republic of Serbia | Belgrade | Lalic Nebojsa M |
| Russia | Yoshkar-Ola | Dubinin M |
| Russia | Moscow | Vinogradov Andrey D |
| Russia | Moscow | Pavlova Nadia |
| Serbia | Belgrade | Savkovic U |
| Serbia | Belgrade | Mandic M |
| Serbia | Belgrade | Djordjevic M |
| Serbia | Belgrade | Misirkic Marjanovic Maja |
| Singapore | Singapore | Hausenloy Derek J |
| Singapore | Singapore | Singh Brijesh Kumar |
| Slovakia | Bratislava 45 | Ferko Miroslav |
| Slovakia | Košice | Cizmarova Beata |
| Slovakia | Bratislava | Sumbalova Zuzana |
| Spain | Valencia | Casado Pinna Marta |
| Spain | Barcelona | Goncalo Teixeira da Silva Rui |
| Spain | Barcelona | Bravo-Sagua Roberto |
| Spain | Barcelona | Zorzano Antonio |
| Spain | Sevilla | Bustos Matilde |
| Spain | Barcelona | Gil J |
| Spain | Barcelona | Gama Perez Pau |
| Spain | Granada | Quiles JL |
| Spain | Barcelona | Garcia-Roves Pablo Miguel |
| Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
| Spain | Barcelona | Perales Jose Carles |
| Spain | Madrid | Hernansanz-Agustin Pablo |
| Spain | Barcelona | De la Torre Lara J |
| Spain | A Coruña | Mayan MD |
| Spain | A Coruña | Blanco FJ |
| Spain | Granada | Hidalgo-Gutierrez A |
| Spain | Madrid | Moran M |
| Spain | Salamanca | Almeida Angeles |
| Spain | Madrid | Aragones Lopez J |
| Spain | Las Palmas de Gran Canaria | Calbet Jose AL |
| Spain | Madrid | Rial Eduardo |
| Sweden | Stockholm | Larsen Filip J |
| Sweden | Stockholm | Cardinale Daniele A |
| Sweden | Stockholm | Shabalina Irina G |
| Sweden | Gothenburg | Bhuvanachandran NS |
| Sweden | Stockholm | Nedergaard J |
| Sweden | Lund | Ehinger Johannes K |
| Switzerland | Lausanne | Canto Alvarez Carles |
| Switzerland | Lausanne | Gebara E |
| Switzerland | Lausanne | Place Nicolas |
| Switzerland | Bern | Nuoffer Jean-Marc |
| Switzerland | Zurich | Gorr Thomas A |
| Switzerland | Lausanne | Burtscher Johannes |
| Switzerland | Bern | Wyss RK |
| Switzerland | Lausanne | Lagarrigue Sylviane |
| Switzerland | Basel | Fornaro Mara |
| Switzerland | Lausanne | Morato Fornaguera L |
| Switzerland | Lausanne | Donnelly Chris |
| Switzerland | Basel | Bouitbir Jamal |
| Switzerland | Zürich | Zaugg Kathrin |
| Switzerland | Lausanne | Sandi Carmen |
| Switzerland | Lausanne | Weger M |
| Switzerland | Lausanne | Zanou Nadege |
| Switzerland | Lausanne | Zalachoras I |
| Switzerland | Lausanne | Amati Francesca |
| Taiwan | Changhua City | Wei Yau-Huei |
| The Netherlands | Groningen | Bakker Barbara M |
| The Netherlands | BT Amsterdam | Wuest Rob CI |
| The Netherlands | Maastricht | Schrauwen Patrick |
| The Netherlands | Maastricht | Nabben Miranda |
| The Netherlands | Delft | McMillan Duncan GG |
| UK | London | Rodriguez Enrique |
| UK | Cambridge | Brown Guy C |
| UK | London | Lane Nick |
| UK | Canterbury, Kent | Gourlay Campbell W |
| UK | London | Bettinazzi Stefano |
| USA | Gainesville | Neyroud D |
| USA | St. Louis | Abumrad Nada A |
| USA | Rochester | Lanza Ian R |
| USA | Winston-Salem | Ahn Bumsoo |
| USA | Birmingham | Moellering Douglas R |
| USA | Iowa City | Wagner Brett A |
| USA | Kansas City | Swerdlow Russell H |
| USA | Colorado Springs | Jacobs RA |
| USA | Milwaukee | Kandel Sunil Mani |
| USA | Stillwater | Davis Michael S |
| USA | San Diego; La Jolla | Molina Anthony JA |
| USA | Jupiter | Trivigno Catherine |
| USA | New Haven | Cavalcanti-de-Albuquerque Joao Paulo |
| USA | Fayetteville | Iyer Shilpa |
| USA | St. Louis | Pietka Terri A |
| USA | Baton Rouge | Irving Brian A |
| USA | Kansas City | Thyfault John P |
| USA | Philadelphia | Jang David H |
| USA | Davis | Adams Sean H |
| USA | San Diego | Cannon Daniel T |
| USA | Fayetteville | Rao RR |
| USA | Baton Rouge | Hand Steven C |
| USA | Corvallis | Newsom Sean A |
| USA | Columbus | Sims Carrie A |
| USA | Winston-Salem | Gonzalez-Armenta Jenny L |
| USA | Iowa City | Chaurasia Bhagirath |
| USA | Fairbanks | O'Brien Kristin |
| USA | Philadelphia | Falk Marni J |
| USA | Nashville | Wasserman David H |
| USA | Baltimore | Gonzalez-Freire Marta |
| USA | Denton | Dzialowski Edward M |
| USA | Greenville | Neufer P Darrell |
| USA | San Diego | Patel Hemal H |
| USA | Orlando | Coen Paul M |
| USA | Puskarich Michael A | |
| USA | Cleveland | Tandler Bernard |
| USA | Orlando | Goodpaster Bret H |
| USA | Berkeley | Williams Caroline M |
| USA | Louisville | Menze Michael A |
| USA | Denver | Van Hove Johan |
| USA | El Paso | Bajpeyi Sudip |
| USA | La Jolla | Valentine Joseph Marco |
| USA | Akron | Cohen Bruce H |
| USA | Warminster | Orynbayeva Zulfiya |
| USA | East Lansing | Zhang Yizhu |
| USA | Davis | Hellgren Kim T |
| USA | Philadelphia | Ganetzky Rebecca |
| USA | Pittsburgh | Jurczak Michael J |
| USA | Aurora | Sparagna Genevieve C |
| USA | Bethesda | Fessel Joshua Patrick |
| USA | Cambridge | Wohlwend Martin |
| USA | Los Angeles | Kwast Kurt E |
| USA | Needham | Brown David A |
| USA | Provo | Hancock Chad R |
| USA | Fairbanks | Coker Robert H |
| USA | Pittsburgh | Prochownik Edward V |
| USA | Galveston | Durham William J |
| USA | Miami | Fontanesi F |
| USA | Little Rock | MacMillan-Crow Lee Ann |
| USA | Boston | Kristal Bruce S |
| USA | Aurora | Ford Ellen |
| USA | Orlando | Pino Maria F |
| USA | Ann Arbor | Tyrrell Daniel J |
| USA | Louisville | Skolik Robert A |
| USA | Little Rock | Borsheim Elisabet |
| USA | Wakefield | Varricchio Frederick |
| USA | Aurora | Moreau Kerrie |
| USA | Fort Collins | Li Puma Lance C |
| USA | La Jolla | Schilling Jan M |
| USA | Omaha | Kim Julian KS |
| USA | Milwaukee | Dash Ranjan K |
| USA | Fort Collins | Chicco Adam J |
| USA | East Lansing | Bazil Jason N |
| USA | Portland | Garlid Keith D |
| USA | Torrance | Rossiter Harry B |
| USA | Cleveland | Hoppel Charles L |
| USA | Bethesda | Sharma Pushpa |
| USA | Winston-Salem | Tomar Dhanendra |
| USA | Orlando | Sparks Lauren M |
| USA | Little Rock | Parajuli Nirmala |
| USA | Chicago | Dayanidhi Sudarshan |
| USA | Rochester | Nair K Sreekumaran |
| USA | Durham | Li Pingan Andy |
| USA | New York | Galkin Alexander |
| ... further results | ||
Task groups
- TG leaders: suggestions discussed at the MitoFit Science Camp 2016 Kuehtai AT, and updated according to the MITOEAGLE MC Meeting 2016 Brussels BE.
- TG participants: summary of the feedback received.
TG1.1 Coupling states and coupling control ratios: consensus statements
- TG leaders: Renner-Sattler Kathrin DE / Gnaiger Erich AT / Hoppel Charles L US
- TG participants:
TG1.2 SUIT protocols: nomenclature consistent with concept of database
- TG leader: Doerrier Velasco Carolina A AT, Gnaiger Erich AT
- TG participants: Attimonelli Marcella IT, Krako Nina RS, Vendelin Marko EE
TG1.3 Experimental details in SUIT
- TG leaders: Chicco Adam J US/ Hickey Anthony J NZ/ Doerrier Velasco Carolina A AT
- TG participants: Casado Pinna Marta ES, Krako Nina RS
TG1.4 Instrumental platforms: comparisons
- TG leaders: Elmer Eskil SE (suggested), Molina Anthony JA US
- TG participants: Canto Carles CH (suggested), Genova Maria Luisa IT, Tronstad Karl Johan NO (suggested), Vendelin Marko EE, Zorzano Antonio ES (suggested)
TG1.5 Knowledge management system
- TG leaders: Drinnan Michael UK / Plattner Christina AT
- TG participants: Vendelin Marko EE, Garcia-Roves Pablo ES
TG1.6 Proficiency test
- TG leaders: Gnaiger Erich AT/ Doerrier Velasco Carolina A AT
- TG participants: Genova Maria Luisa IT, Plattner Christina AT
TG1.7 Documentation
- TG leaders: Arnould Thierry BE/ Votion Dominique-Marie BE
- TG participants:
TG1.8 Translation to community in general
- TG leader: Makrecka-Kuka Marina LV (Dissemination Coordinator), Rattan Suresh DK
- TG participants: Krako Nina RS, Irving Brian A US
TG1.9 Dissemination Scientific community
- TG leaders: Makrecka-Kuka Marina LV / Gnaiger Erich AT
- TG participants: Ali Sameh S EG, Cervinkova Zuzana CZ, Chicco Adam J US, Coen Paul M US, Eide Lars NO, Garcia-Roves Pablo M ES, Gasparre Giuseppe IT, Genova Maria Luisa IT, Haendeler Judith DE, Iyer Shilpa US, Krako Nina RS, Laner Verena AT, Mayan Maria D ES, Mishmar Dan IL, Nehlin Jan DK, Palmeira Carlos PT, Paterlini Serena IT, Porcelli Anna Maria IT, Rao Raj US, Schaefer Patrick DE, Tronstad Karl Johan NO, Trougakos Ioannis GR, Volani Chiara AT, Votion Dominique-Marie BE
TG1.10 Training, Short Term Scientific Missions
- TG leader: Labieniec-Watala Magdalena PL, Klaus Susanne DE
- TG participants: Ali Sameh S EG, Cervinkova Zuzana CZ, Chicco Adam J US, Coen Paul M US, Eide Lars NO, Garcia-Roves Pablo M ES, Gasparre Giuseppe IT, Genova Maria Luisa IT, Haendeler Judith DE, Iyer Shilpa US, Krako Nina RS, Mayan Maria D ES, Mishmar Dan IL, Nehlin Jan DK, Palmeira Carlos PT, Paterlini Serena IT, Porcelli Anna Maria IT, Rao Raj US, Schaefer Patrick DE, Tronstad Karl Johan NO, Trougakos Ioannis GR, Volani Chiara AT, Votion Dominique-Marie BE
Comments and discussion
- We are mostly interested in two WG: (1) Since we are working mostly with heart and muscles, we are interested in WG 2 - MITOEAGLE data repository in muscle and other tissues. (2) WG 1 - Standard operating procedures and user requirement document: Protocols, terminology, documentation. Since we have an expertise in fatty acid metabolism, we are specially interested in experimental protocols for the evaluation of mitochondrial capacities regarding fatty acid metabolism. Of course our parts of the WG are also in our interest. - Marina Makrecka-Kuka, Edgars Liepinsh (2016).
- My role in Mito-EAGLE would be mainly in WG 2 and 4, with a focus on skeletal and cardiac muscle and cultured cardiomyocytes and endothelial cells. I would particularly like to bridge the gap between the group leaders and young investigators, trying to represent the young people who are the active users of the OROBOROS equipment. This will fit in nicely with the goals in WG1. ... Major congratulations on obtaining this beautiful collaborative grant! Very glad to see this network getting the recognition it deserves! Looking forward to being part of this! - Rob C Wuest (2016).
- Please, find enclosed my declaration to participate with my team in the following workgroups: WG1, WG2, WG3, WG4. We are now involved and have been in not a distant past – in studying of the following cell /tissue types:
- Blood cells, with particular emphasis on platelets and lymphocytes – isolated from animals and humans.
- Endothelial cells, fibroblasts, neurocytes (neuroblastoma), astrocytes, cancer cell lines – predominantly cultured.
- Hepatocytes, cardiomyocytes, cell isolated from brain – isolated from lab animals. - Cezary Watala (2016).
- I have already contacted the portuguese CNC to nominate me for the MC of theaction. Concerning my active participation, taking into account the type of work we do in my lab I could be included in all WGs. As you know we work with mitochondria from liver, muscle and fat, as well as cultured cells from different origins (WG2-4). The elaboration of SOPs (WG1) we can certainly contribute (see the book I edited on Mitochondrial Bioenergetics and in which you contribute a chapter). I believe that everybody can be part of the WG5, but as the coordinators of the action, you and Sandra should decide what is best for the success of the action. - Carlos Palmeira (2016).
- I would propose my participation in the WG1: Standard operating procedures and user requirement document: Protocols, terminology, documentation, considering that the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. I could give my active contribution in the phase of making platform that will aggregate the Action’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases in particular tasks:
- Develop reccomendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalised service for data management, data mining and health-care conforming standards of data interpretation
- Provide a summary on strategic dissemination and an education programme for MITOEAGLE.
- Considering that we are in process of buying the oxygraph, thus broading capacities of my lab, I would be happy to open the door of my new lab for the innovative inter-laboratory ring test experiments and together with my team contribute in that phase of the MITOEAGLE Action. I look forward to the establishment of MitoGlobal EAGLE network where, as stated in the MoU, researchers will collaborate on mapping mitochondrial physiology and medicine. The creation of such a unique well-coordinated network of senior researchers and young investigators will definitely put an added value to existing data and publications, will increase quality and validity of the research, will bring new possibilities of data sharing and data mining and will hopefully bring us to the era of more economical research by saving resources through standardised and harmnonised protocols, where the measuremnts will not be wasted due to lack of comparability. - Nebojsa Lalic (2016).
- Our research interest is oriented to reproductive endocrinology; especially to mechanisms that govern steroidogenesis of testicular Leydig cells in disturbed homeostasis. We use several in vivo models:
- stress -/+ systemic or intratesticular blockade of different stress receptors (alpha1ADR; betaADR; GR)
- hypogonadal-hypogonadism -/+ testosterone-replacement therapy
- androgens anabolics treatment -/+ blockade od AR
- aged rats -/+ Viagra-treatment
- biological clock - Silvana Andric (2016).
- I took a look to the MoU and the different working groups. You know well my research focus and having this into account I am interested in actions related to Working group 1 (Respirometric reference protocols, Interlaboratory proficiency test, Instrumental platforms). Working group 2 and 3: I have generated multiple mitochondrial respiration data sets in different mouse tissues (mainly in skeletal muscle, hypothalamus, liver and white adipose tissue). Currently we are also performing these assays in white adipose tissue from human patients. Hence I am interested in all milestones of WG 2 and 3. WG 5 is related to WG 1 and as you know I have interest in training, dissemination and also together with my collaborators (if needed) we could help with data sets and a data interpretation. Overall, I have a general interest on this Action and my active role in MITOEAGLE could be discussed. I could be of help participating in some of the tasks where my expertise and interest fit best. We will be forming and interdisciplinary research group with four group leaders: JC Perales, R Guimera, M Sales-Pardo and PM Garcia-Roves with two different affiliations, University of Barcelona (JCP and PMG-R - Energy metabolism) and University Rovira I Virgili (RG and MS – Data base, statistics, data mining). - Pablo M Garcia-Roves (2016).
- I will be pleased to contribute to the tasks of WG1 by developing interlaboratory proficiency tests and instrumental platform comparison. Moreover, as a (potential) MC member of MitoEAGLE I can also offer visibility to the Action itself (i.e. WG5: MITOEAGLE dissemination and training, according to mitoglobal.org webpages) by establishing new interactions with the International Coenzyme Q10 Association (ICQA) where I act as a member of the Executive Committee. ICQA organizes biennal conferences on new highlights of Coenzyme Q research. In 2015, we held the conference in Bologna (http://conference2015.wix.com/icqaconference) and we included in the program also some scientific sessions dedicated to mitochondrial bioenergetics and diseases. I will be pleased to enforce cooperation between ICQA and MitoEAGLE. - Maria Luisa Genova (2016).
- We found the scientific content and objectives of the call very interesting and compatible with our wide expertise on oxysterol analysis in food and biological systems. Our group has a large experience on lipid composition and their degradation products (volatile and non-volatile), as well as on sensory analysis. Moreover, we are currently involved in the H2020-GERI-2014-1 project "PLOTINA" ("Promoting gender balance and inclusion in research, innovation and training") with Prof. Gallina Toschi (in cc) as coordinator; the project actually includes gendered case studies where gender and sex variables are considered. You can find further information about our expertises, publications and the PLOTINA project at the following webpages: https://www.unibo.it/sitoweb/maria.rodriguez/, https://www.unibo.it/sitoweb/tullia.gallinatoschi/, http://www.unibo.it/en/research/projects-and-initiatives/research-projects-horizon-2020-1/plotina - Maria Teresa Rodriguez Estrada (2016).
- As you know, our current/developing interests are with "evolution" (species comparisons) and "environment" (hypoxia and cold exposure), but have also experience and interest in assessing effects of "lifestyle" (diet, exercise) and "aging" as well (in rodents). - Adam J Chicco (2016).
- Congratulations on the considerable progress with MITOEAGLE! I am keen to participate in this exciting project and I’m sure I can make a valuable contribution to it's success. I have extensive experience performing respirometry assays in human muscle biopsies, myoblasts/tubes and mouse tissues in the contexts of aging, obesity, exercise and inactivity. I feel that I could contribute to any of the five working groups, but particularly to WG2, WG4, and WG5. I am also happy and willing to serve on the Management Committee if needed. Again, congratulations on organizing such an exciting project. - Paul M Coen (2016).
- I would be interested to be involved with WG1 and WG2. In particular, I am interested in contributing to developing protocols for multicenter quality control among labs, and for permeabilized muscle bundle respirometry. - Harry B Rossiter (2016).
- I think that we could contribute via participation in the evaluation of the protocols, and helping to develop them further. In addition, we have been working on the establishing a database that would allow us to keep protocol details, cell isolation and several other aspects. There is nothing very advanced, but we could contribute to establishing such data collection practice. In addition, we, as opposed to many others, happen to use Strathkelvin respirometer. This alternative hardware would allow us to verify whether the protocols work on them as well. - Marko Vendelin (2016).
Projects in progress
- Interlaboratory proficiency ring test
- Testing of software components
- Concepts and terminology on mitochondrial physiology
- Procedures and experimental protocols for the evaluation of mitochondrial capacities, library of protocols
- Data management system (DMS)
- Database
Next steps
- » MITOEAGLE 2016 Verona IT
- » MITOEAGLE 2017 Barcelona ES
- Best practices for implementation of analytical and process standards.
References
- General requirements for the competence of testing and calibration laboratories » ISO/IEC 17025:2005
