Sodium phosphate buffer: Difference between revisions
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* '''Oxygen solubility factor''' of Na-PB = '''0.95''' | * '''Oxygen solubility factor''' of Na-PB = '''0.95''' | ||
== Na-PB (50 mM, pH 7.1) | == Na-PB (50 mM, pH 7.1)== | ||
Dissolve 3.9 g H<sub>2</sub>NaO<sub>4</sub>P x 2 H<sub>2</sub>O in 500 ml a. dest and adjust pH with 10 M NaOH to 7.1. | Dissolve 3.9 g H<sub>2</sub>NaO<sub>4</sub>P x 2 H<sub>2</sub>O in 500 ml a. dest and adjust pH with 10 M NaOH to 7.1. | ||
* '''pH''' of Na-PB = '''7.1''' | * '''pH''' of Na-PB = '''7.1''' | ||
Revision as of 13:45, 23 April 2012
- high-resolution terminology - matching measurements at high-resolution
Sodium phosphate buffer
Description
Sodium phosphate buffer, Na-PB, for HRR with freeze-dried baker´s yeast
Abbreviation: Na-PB
MitoPedia methods:
Respirometry
MitoPedia topics:
Media for respirometry
Application in HRR
- experimental temperature = 28°C
- Oxygen solubility factor of Na-PB = 0.95
Na-PB (50 mM, pH 7.1)
Dissolve 3.9 g H2NaO4P x 2 H2O in 500 ml a. dest and adjust pH with 10 M NaOH to 7.1.
- pH of Na-PB = 7.1
