Difference between revisions of "Coupling-control ratio"
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{{MitoPedia | {{MitoPedia | ||
|abbr=''CCR'' | |abbr=''CCR'' | ||
|description='''Coupling control ratios''', ''CCR'', are [[flux control ratio]]s, ''FCR'', at a constant mitochondrial [[pathway control state]]. In mitochondrial preparations, there are three well-defined coupling states of respiration, ''L'', ''P'', ''E'' ([[LEAK]], [[OXPHOS]], [[ET-pathway]]). In | |description='''Coupling-control ratios''', ''CCR'', are [[flux control ratio]]s, ''FCR'', at a constant mitochondrial [[pathway-control state]]. In mitochondrial preparations, there are three well-defined coupling states of respiration, ''L'', ''P'', ''E'' ([[LEAK]], [[OXPHOS]], [[ET-pathway]]). In living cells, the OXPHOS state cannot be induced, but a [[ROUTINE]] state of respiration, ''R'', can be measured. The reference state, ''J''<sub>ref</sub>, is defined by taking ''J''<sub>ref</sub> as the maximum flux, i.e. flux in the ET state, ''E'', such that the lower and upper limits of the ''CCR'' are defined as 0.0 and 1.0. Then there are two mitochondrial ''CCR'', [[L/E|''L/E'']] and [[P/E|''P/E'']], and two ''CCR'' for living cells, [[L/E|''L/E'']] and [[R/E control ratio|''R/E'']]. | ||
|info=[[Flux control ratio]] | |info=[[Flux control ratio]] | ||
}} | }} | ||
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== Coupling control ratios related to excess capacities == | == Coupling control ratios related to excess capacities == | ||
:::* [[OXPHOS control ratio]], ''P/E'' | :::* [[OXPHOS-control ratio]], ''P/E'' | ||
:::: [[Excess E-P capacity |Excess ''E-P'' capacity]], ''ExP = E-P'' | :::: [[Excess E-P capacity |Excess ''E-P'' capacity]], ''ExP = E-P'' | ||
:::: [[Excess E-P capacity factor |Excess ''E-P'' capacity factor]], ''j<sub>ExP</sub>'' = ''ExP''/''E'' = 1-''P/E'' | :::: [[Excess E-P capacity factor |Excess ''E-P'' capacity factor]], ''j<sub>ExP</sub>'' = ''ExP''/''E'' = 1-''P/E'' | ||
:::* [[ROUTINE control ratio]], ''R/E'' | :::* [[ROUTINE-control ratio]], ''R/E'' | ||
:::: [[Excess E-R capacity |Excess ''E-R'' capacity]], ''ExR = E-R'' | :::: [[Excess E-R capacity |Excess ''E-R'' capacity]], ''ExR = E-R'' | ||
:::: [[Excess E-R capacity factor |Excess ''E-R'' capacity factor]], ''j<sub>ExR</sub>'' = ''ExR''/''E'' = 1-''R/E'' | :::: [[Excess E-R capacity factor |Excess ''E-R'' capacity factor]], ''j<sub>ExR</sub>'' = ''ExR''/''E'' = 1-''R/E'' |
Revision as of 11:58, 2 June 2020
Description
Coupling-control ratios, CCR, are flux control ratios, FCR, at a constant mitochondrial pathway-control state. In mitochondrial preparations, there are three well-defined coupling states of respiration, L, P, E (LEAK, OXPHOS, ET-pathway). In living cells, the OXPHOS state cannot be induced, but a ROUTINE state of respiration, R, can be measured. The reference state, Jref, is defined by taking Jref as the maximum flux, i.e. flux in the ET state, E, such that the lower and upper limits of the CCR are defined as 0.0 and 1.0. Then there are two mitochondrial CCR, L/E and P/E, and two CCR for living cells, L/E and R/E.
Abbreviation: CCR
Reference: Flux control ratio
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- Free OXPHOS capacity, ≈P = P-L
- OXPHOS coupling efficiency, j≈P = ≈P/P = 1-L/P
- Free ROUTINE activity, ≈R = R-L
- ROUTINE coupling efficiency, j≈R = ≈R/R = 1-L/R
- LEAK control ratio, L/E
- Free ET-capacity, ≈E = E-L
- ET-coupling efficiency, j≈E = ≈E/E = 1-L/E
- netOXPHOS control ratio, ≈P/E=(P-L)/E
- Free OXPHOS capacity, ≈P = P-L
- netROUTINE control ratio, ≈R/E=(R-L)/E
- Free ROUTINE activity, ≈R = R-L
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- More details » ET-coupling efficiency
- OXPHOS-control ratio, P/E
- Excess E-P capacity, ExP = E-P
- Excess E-P capacity factor, jExP = ExP/E = 1-P/E
- Excess E-R capacity, ExR = E-R
- Excess E-R capacity factor, jExR = ExR/E = 1-R/E
Compare
- » Coupling control factor, CCF = 1-CCR
- » Respiratory acceptor control ratio, RCR
References
- Bioblast links: Normalization - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Quantities for normalization
- » Count in contrast to Number
- » Mitochondrial marker
- » O2k-Protocols: mitochondrial and marker-enzymes
- » Citrate synthase activity
- Quantities for normalization
- General
- Related keyword lists
MitoPedia concepts:
Respiratory control ratio
MitoPedia methods:
Respirometry