Flux baseline correction: Difference between revisions

Latest revision as of 11:50, 25 March 2022

Flux baseline correction

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Description

Flux baseline correction provides the option to display the plot and all values of the flux (or flow, or flux control ratio) as the total flux, J, minus a baseline flux, J₀.

J_V(bc) = J_V - J_V₀
J_V = (dc/dt) · ν^-1 · SF - J°_V

For the oxygen channel, J_V is O2 flux per volume [pmol/(s·ml)] (or volume-specific O₂ flux), c is the oxygen concentration [nmol/ml = µmol/l = µM], dc/dt is the (positive) slope of oxygen concentration over time [nmol/(s · ml)], ν^-1 = -1 is the stoichiometric coefficient for the reaction of oxygen consumption (oxygen is removed in the chemical reaction, thus the stoichiometric coefficient is negative, expressing oxygen flux as the negative slope), SF=1,000 is the scaling factor (converting units for the amount of oxygen from nmol to pmol), and J°_V is the volume-specific background oxygen flux (Instrumental background oxygen flux). Further details: Flux / Slope.

Abbreviation: bc

MitoPedia methods: Respirometry, Fluorometry

MitoPedia O2k and high-resolution respirometry: DatLab

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 |abbr=bc
 |description='''Flux baseline correction''' provides the option to display the plot and all values of the [[flux]] (or [[flow]], or [[flux control ratio]]) as the total flux, ''J'', minus a baseline flux, ''J''<sub>0</sub>.
   ''J<sub>V</sub>''(bc) = ''J<sub>V</sub>'' - ''J<sub>V</sub>''<sub>0</sub>
   ''J<sub>V</sub>'' = (d''c''/d''t'') · ''ν''<sup>-1</sup> · ''SF'' - ''J°<sub>V</sub>''
-For the oxygen channel, ''J<sub>V</sub>'' is O2 flux per volume [pmol/(s·ml)] (or volume-specific O<sub>2</sub> flux), ''c'' is the oxygen conentration [nmol/ml = µmol/l = µM], d''c''/d''t'' is the (positive) slope of oxygen concentration over time [nmol/(s · ml)], ''ν''<sup>-1</sup> = -1 is the stoichiometric coefficient for the reaction of oxygen consumption (oxygen is removed in the chemical reaction, thus the stoichiometric coefficient is negative, expressing oxygen flux as the negative slope), ''SF''=1,000 is the scaling factor (converting units for the amount of oxygen from nmol to pmol), and ''J°<sub>V</sub>'' is the volume-specific background oxygen flux ([[Instrumental background oxygen flux]]). ''Further details'': [[Flux / Slope]].
+For the oxygen channel, ''J<sub>V</sub>'' is O2 flux per volume [pmol/(s·ml)] (or volume-specific O<sub>2</sub> flux), ''c'' is the oxygen concentration [nmol/ml = µmol/l = µM], d''c''/d''t'' is the (positive) slope of oxygen concentration over time [nmol/(s · ml)], ''ν''<sup>-1</sup> = -1 is the stoichiometric coefficient for the reaction of oxygen consumption (oxygen is removed in the chemical reaction, thus the stoichiometric coefficient is negative, expressing oxygen flux as the negative slope), ''SF''=1,000 is the scaling factor (converting units for the amount of oxygen from nmol to pmol), and ''J°<sub>V</sub>'' is the volume-specific background oxygen flux ([[Instrumental background oxygen flux]]). ''Further details'': [[Flux / Slope]].
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