Description
Flux control ratios (FCR), are ratios of oxygen flux in different respiratory control states, normalized for maximum flux in a common reference state, to obtain theoretical lower and upper limits of 0.0 and 1.0 (0% and 100%).
For a given protocol or set of respiratory protocols, flux control ratios provide a fingerprint of coupling and substrate control independent of (i) mt-content in cells or tissues, (ii) purification in preparations of isolated mitochondria, and (iii) assay conditions for determination of tissue mass or mt-markers external to a respiratory protocol (CS, protein, stereology, etc.). FCR obtained from a single respirometric incubation with sequential titrations (sequential protocol; SUIT protocol) provide an internal normalization, expressing respiratory control independent of mitochondrial content and thus independent of a marker for mitochondrial amount. FCR obtained from separate (parallel) protocols depend on equal distribution of subsamples obtained from a homogenous mt-preparation or determination of a common mitochondrial marker.
Abbreviation: FCR
Reference: Gnaiger 2014 MitoPathways, Gnaiger 2009 Int J Biochem Cell Biol, Doerrier 2018 Methods Mol Biol
Flux control efficiency: normalization of mitochondrial respiration
- Β» More details: Flux control efficiency
DatLab
Unknown sample concentration and normalization per unit sample [x]
- In the DatLab 7.4 Excel template for oxygen flux analysis (O2 analysis template DL7.4):
- If the sample concentration is not yet known, the box βKnown sample concentrationβ can be unchecked, and the concentration will be considered by default as 1, with units [xΒ·mL-1]. In this way, flux can be normalized and FCRs can be obtained even if the sample concentration is unknown.
- Β» Read also: Extensive quantity; BEC 2020.1
- Β» More details: MiPNet24.06 Oxygen flux analysis - DatLab 7.4
FCR in DatLab plot
- The entire plot of oxygen flux can be converted to a FCR. Click on 'Flux/Slope' in the DatLab pull-down menu. Select chamber A or B 'O2 slope'. Select 'Flux control ratio, FCR' and select the mark that corresponds to the reference state. Change the scale under 'Graph/Scaling' (or press F6).
- Β» More details: Flux/Slope in DatLab
References
Bioblast link | Reference | Year |
---|---|---|
Doerrier 2018 Methods Mol Biol | Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, MΓ©szΓ‘ros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. https://doi.org/10.1007/978-1-4939-7831-1_3 | 2018 |
Gnaiger 2009 Int J Biochem Cell Biol | Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41:1837-45. https://doi.org/10.1016/j.biocel.2009.03.013 | 2009 |
Gnaiger 2020 BEC MitoPathways | Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002 | 2020 |
BEC 2020.1 doi10.26124bec2020-0001.v1 | Gnaiger E et al β MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1 | 2020 |
- Bioblast links: Normalization - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Rate
- Β» Normalization of rate
- Β» Flow
- Β» Oxygen flow
- Β» Flux
- Β» Oxygen flux
- Β» Flux control ratio
- Β» Coupling-control ratio
- Β» Pathway control ratio
- Β» Flux control efficiency
- Rate
- Quantities for normalization
- Β» Count in contrast to Number
- Β» Mitochondrial marker
- Β» O2k-Protocols: mitochondrial and marker-enzymes
- Β» Citrate synthase activity
- Quantities for normalization
- General
- Β» Extensive quantity
- Β» Specific quantity
- Β» Advancement
- Β» Motive unit
- Β» Iconic symbols
- General
- Related keyword lists
MitoPedia concepts:
Respiratory control ratio,
SUIT concept
MitoPedia methods:
Respirometry
MitoPedia O2k and high-resolution respirometry:
DatLab