|
Β |
(57 intermediate revisions by 11 users not shown) |
Line 1: |
Line 1: |
| {{OROBOROS header page name}} | | {{Template:OROBOROS support page name}} |
| {{Publication | | {{Publication |
| |title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. | | |title=[[Image:O2k-Manual.jpg|right|70px|link=O2k-Manual|O2k-Manual]]O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. |
| |info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/7/77/MiPNet06.03_POS-Calibration-SOP.pdf |Bioblast pdf]] |authors=Oroboros | | |info=[[File:PDF.jpg|100px|link=https://wiki.oroboros.at/images/7/77/MiPNet06.03_POS-Calibration-SOP.pdf |Bioblast pdf]] Β» [http://www.bioblast.at/index.php/File:MiPNet06.03_POS-Calibration-SOP.pdf Versions] |
| |year=2016-02-09 | | |authors=Oroboros |
| | |year=2023-02-06 |
| |journal=Mitochondr Physiol Network | | |journal=Mitochondr Physiol Network |
| |abstract='''Gnaiger E (2016) O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. Mitochondr Physiol Network 06.03(15):1-8.''' Β» [http://www.bioblast.at/index.php/File:MiPNet06.03_POS-Calibration-SOP.pdf Versions] | | |abstract=Gnaiger E (2023) O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. Mitochondr Physiol Network 06.03(21):1-10. |
| Β | | {{MiPNet pdf page linking to MitoPedia}} |
| '''O2k-Protocols SOP''': High-resolution respirometry (HRR; Oroboros O2k) critically depends on maintenance POS-service and accurate calibration of the polarographic oxygen sensors ([[OroboPOS]], POS). Standard operating procedures (O2k-SOP) are described as part of the [[MitoFit Quality Control System]]. (1) Clean and ready-to-use O2k-Chambers are a basic requirement. (2) Quality control for evaluation of proper POS function (SOP: O2 sensor test including the [[stirrer test]]). (3) Accurate POS calibration ([[air calibration]] and [[zero calibration]]). This is part 1 of O2k Quality Control.
| |
| Β | |
| :Β» O2k-Protocols SOP: O2k Quality Control 2 Β»[[MiPNet14.06 Instrumental O2 background]]
| |
| :Β» O2k-Manual: [[MiPNet19.18D_O2k-calibration]]
| |
| :Β» Product: [[OroboPOS]], [[Oroboros O2k]], [[Oroboros O2k-Catalogue |O2k-Catalogue]]
| |
| :Β» Template: [[File:O2-calibration.xlsx|O2-calibration.xlsx]]
| |
| |mipnetlab=AT_Innsbruck_Oroboros | | |mipnetlab=AT_Innsbruck_Oroboros |
| }} | | }} |
| {{Labeling
| |
| |area=Instruments;methods
| |
| |instruments=Oxygraph-2k, O2k-Protocol
| |
| |additional=O2k-SOP
| |
| }}
| |
| <br />
| |
| [[File:Expand.png|right|45px |Click to expand or collaps]]
| |
| <div class="toccolours mw-collapsible mw-collapsed">
| |
| :::[[File:Questions.jpg|left|24px |Explanation of terms]] '''Β» Keywords'''
| |
| <div class="mw-collapsible-content">
| |
| ::: '''Instrument'''
| |
| ::::Β» [[O2k-Core]]
| |
| ::::Β» [[MiPNet19.18D O2k-calibration |O2k-Manual: O2k-calibration]]
| |
| ::::Β» [[MiPNet19.18B POS-service |O2k-Manual: POS-service]]
| |
| ::::Β» [[MiPNet19.03 O2k-cleaning and ISS |O2k-Protocols SOP: O2k-cleaning]]
| |
| ::::Β» [[OroboPOS]]
| |
| ::::Β» [[Polarographic oxygen sensor]]
| |
| ::::Β» [[Stirrer test]]
| |
|
| |
|
| ::: '''Concept'''
| | == Keywords == |
| ::::Β» [[Air calibration]]
| | {{Template:Keywords: Oxygen signal}} |
| ::::Β» [[Concentration]]
| |
| ::::Β» [[High-resolution respirometry]]
| |
| ::::Β» [[MiPNet06.03 POS-calibration-SOP |O2k-Protocols SOP: POS-calibration]]
| |
| ::::Β» [[MitoFit Quality Control System]]
| |
| ::::Β» [[O2k-SOP |O2k-Protocols SOP]]
| |
| ::::Β» [[Oxygen |Oxygen, dioxygen, O<sub>2</sub>]]
| |
| ::::Β» [[Oxygen sensor test]] | |
| ::::Β» [[Zero calibration]]
| |
| </div>
| |
| </div>
| |
| <br />
| |
| __TOC__
| |
|
| |
|
| {{Technical support integrated}}
| | ::::Β» [[Oxygen calibration - DatLab]] |
| <br>
| |
|
| |
|
| == Standard operating procedure ==
| |
|
| |
|
| ::::The O2 sensor test does not only serve to evaluate the function of the OroboPOS, but many other components of the O2k have to function according to specifications for a high-resolution oxygen signal to be obtained:
| | {{Labeling |
| ::::# [[USB-Cable 2.0\Type A-B]] not properly connected to the O2k and PC or Laptop.
| | |area=Respiration, Instruments;methods |
| ::::# [[O2k-Chamber]] not properly positioned, such that O2 sensors are not connected to the medium.
| | |instruments=Oxygraph-2k, O2k-Manual |
| ::::# [[OroboPOS-Membranes]] defective or not properly applied.
| | |additional=DatLab, O2k-SOP, DL7, Oxygen solubility, DL8, Β |
| ::::# [[OroboPOS-Connector]] contaminated gold contacts; plugs not properly connected to the sockets of the [[O2k-Main Unit]].
| | MitoFit 2021 Dark respiration, MitoFit 2021 Photosynthesis, DL8, PLoSONE2022ace-sce, MitoFit2022QC, POS |
| ::::# OroboPOS contacts not cleaned. In rare cases, [[Pen-Contact Oil]] might be used.
| | }} |
| ::::# [[OroboPOS-Electrolyte Powder]] contaminated, inappropriate water used for dissolving the powder.
| |
| ::::# [[O2-Zero Powder]] not properly handled; confused with [[OroboPOS-Polishing Powder]].
| |
| ::::# [[OroboPOS]] not properly serviced; not properly mounted to the [[OroboPOS-Connector]]; or defective POS head.
| |
| ::::# Room temperature not sufficiently stable.
| |
| ::::# [[Stirrer-Bar\white PVDF\15x6 mm]] not added to the chamber, or stuck and not rotating.
| |
| ::::# [[O2k-Barometric Pressure Transducer]] not properly calibrated.
| |
| ::::# [[O2k-Peltier Temperature Control]] defective electronics.
| |
| ::::# [[O2k-Electromagnetic Stirrer Twin-Control]] defective electronics.
| |
| ::::# [[O2k-Main Basic]] not properly connected; other defective hardware.
| |
| Β | |
| == Trouble shooting ==
| |
| :::'''If the signal remains off scale''' (9.99 V) or very low (< 1 V) at air saturation (25 to 37 Β°C; lower signals at lower temperatures): Many components may be responsible, and an electronic defect of the [[O2k-Main Unit]] must be carefully excluded.
| |
| ::::# Check settings for [[Gain]] (use Gain 1) and [[Polarization voltage]] (use 800 mV). If these settings were incorrect, the off-scale problem may be solved simply by using the standard settings.
| |
| ::::# Empty the chamber with the O2k running and connected to DatLab. Switch the stirrer off. Remove the [[OroboPOS-Connector]] with the attached [[OroboPOS]]. Leave the sensor attached to the POS connector, and the POS connector plugged into the O2k-Main Unit. Record the signal for some minutes. The raw signal should normalize to a value >1 V and <3 V (Gain 1). If so, the O2k-Chamber assembly was problematic (application problem), and re-assembly will solve the problem. Β» [https://www.youtube.com/watch?v=1JffCpapX6A O2k-Videosupport: Insert O2k-Chamber].
| |
| ::::# Remove the sensor head from the sensor connector, which remains plugged into the O2k-Main Unit. If the raw signal is not stable at 0 V, a defect of the [[OroboPOS-Connector]] is indicated.
| |
| ::::# Remove the plug of the [[OroboPOS-Connector]] from the [[O2k-Main Unit]]. A signal of 0.4 V, which remains stable when changing the gain, is a strong indication of an electronic problem in the O2k-Main Unit.
| |
| ::::## For O2k-Series D and higher: The signal should be 0 V, otherwise an electronic problem of the O2k-Main Unit is indicated.
| |
| ::::## For O2k-Series A to C: The signal should be off-scale (+/- 9.99 V), otherwise an electronic problem of the O2k-Main Unit is indicated.
| |
| Β | |
| :::'''If specifications given in the [[MiPNet06.03 POS-calibration-SOP |POS-SOP]] are not obtained''': switch components for locating the problem.
| |
| ::::# Switch the [[OroboPOS]] between O2k-Chambers A and B. This is a good opportunity to clean the gold contacts and apply [[Contact oil]] to the gold pin and thread connecting the [[POS connector]]s and sensors. It is not necessary to remove the seal tip and the mounted membrane from the OroboPOS. When disconnecting a sensor from the POS connector, prevent damage by electrostatic discharge by following the guidelines: [[MiPNet14.01 ESD-damage]].
| |
| ::::# Repeat the O2 sensor test. If the problem moved together with the sensor from one side to the other, the problem is located in the [[OroboPOS]] sensor.
| |
| ::::# If the problem remained on the same side, switch the [[POS connector]] together with the attached sensors between right and left side (A and B).
| |
| ::::# Repeat the O2 sensor test. If the problem moved together with the POS connector, it is located at the [[OroboPOS-Connector]].
| |
| Β | |
| == Oxygen solubility factor ==
| |
| [[Image:MiPNet06.03_Table.jpg|centre|frame|<sup>c</sup>O2* is the oxygen concentration at thermodynamic equilibrium with air under standard conditions of 100 kPa barometric pressure. ''F''<sub>M</sub> has been determined for MiR05 at 30 Β°C and 37 Β°C, and is assumed to be identical at 25 Β°C.]]
| |
| Β | |
| == Instrumental DL-Protocol ==
| |
| Β | |
| Instrumental [[DL-Protocols]] are used for calibrations and instrumental quality control, typically without experimental sample in the incubation medium.
| |
| Β | |
| {| class="wikitable" border="1"
| |
| |-
| |
| ! DL-Protocol
| |
| ! Description
| |
| ! DLP File
| |
| Β | |
| |-
| |
| | O2-calibration air
| |
| | OroboPos sensor test and calibration at air saturation
| |
| | O2-calibration_air.DLP
| |
| |}
| |
| Β | |
| A full list of DL-Protocols (Instrumental DL-Protocols and SUIT DL-Protocols) is displayedΒ in [[DL-Protocols]].
| |
| Β | |
| == References ==
| |
| ::::* Forstner H, Gnaiger E (1983) Calculation of equilibrium oxygen concentration. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York:321-33. - [[Forstner_1983_POS |Β»Bioblast link]]
| |
| ::::* [[Gnaiger 2008 POS]]
| |
| ::::* [[MiPNet19.18D O2k-calibration]]
| |
| Β | |
| ::: '''This article is cited in'''
| |
| ::::* Rodriguez JV, Pizarro MD, Scandizzi AL, Guibert EE, Almada LL, Mamprin ME (2008) Construction and performance of a minibioreactor suitable as experimental bioartificial liver. Artif Organs 32:323-8. [http://www.ncbi.nlm.nih.gov/pubmed/18370948 Β»PubMed 18370948]
| |
| Β | |
| Β | |
| Β | |
| [[File:O2k-QCS.jpg|350px|O2k-QCS]] Next step - O2k Quality Control 2: [[MiPNet14.06 Instrumental O2 background]]
| |
| Β | |
| Β | |
| [[Category:Technical service]]
| |