N'Guessan 2004 Mol Cell Biochem

N'Guessan B, Zoll J, Ribera F, Ponsot E, Lampert E, Ventura-Clapier R, Veksler V, Mettauer B (2004) Evaluation of quantitative and qualitative aspects of mitochondrial function in human skeletal and cardiac muscles. Mol Cell Biochem 256-257:267-80.

» PMID: 14977187

N'Guessan B, Zoll J, Ribera F, Ponsot E, Lampert E, Ventura-Clapier R, Veksler V, Mettauer B (2004) Mol Cell Biochem

Abstract: Techniques and protocols of assessment of mitochondrial properties are of physiological and physiopathological important significance. A precise knowledge of the advantages and limitations of the different protocols used to investigate the mitochondrial function, is therefore necessary. This report presents examples of how the skinned (or permeabilized) fibers technique could be applied for the polarographic determination of the actual quantitative and qualitative aspects of mitochondrial function in human muscle samples. We described and compared the main available respiration protocols in order to sort out which protocol seems more appropriate for the characterization of mitochondrial properties according to the questions under consideration: quantitative determination of oxidative capacities of a given muscle, characterization of the pattern of control of mitochondrial respiration, or assessment of a mitochondrial defect at the level of the respiratory chain complexes. We showed that while protocol A, using only two levels of the phosphate acceptor adenosine diphosphate (ADP) concentration and the adjunction of creatine, could be used for the determination of quantitative changes in very small amount of muscle samples, the ADP sensitivity of mitochondrial respiration was underestimated by this protocol in muscles with high oxidative capacities. The actual apparent Km for ADP and the role of functional activation of miCK in ATP production and energy transfer in oxidative muscles, are well-assessed by protocol B (in the absence of creatine) together with protocol C (in the presence of creatine) that use increasing concentrations of ADP ranging from 2.5-2000 microM. Protocol D is well-adapted to investigate the potential changes at different levels of the respiratory chain, by the use of specific substrates and inhibitors. As can be seen from the present data and the current review of previous reports in the literature, a standardization of the respiration protocols is needed for useful comparisons between studies.

• Bioblast editor: Gnaiger E

MitoEAGLE V_O2max/BME data base

• Human vastus lateralis
• 4 females & males
• 47.4 years
• Active, regular endurance training without being involved in competition
• H
• M
• BME
• BMI
• V_O2max/BM = 44.6 mL·min^-1·kg^-1 (= V_O2peak/BM/0.93)
• Permeabilized muscle fibres; 22 °C; GM_P; m_d; conversions: Gnaiger 2009 Int J Biochem Cell Biol
• J_O2,P(NS) = 107 µmol·s^-1·kg^-1 wet muscle mass (37 °C)

• J_O2,P(GM) = 78 µmol·s^-1·kg^-1 wet muscle mass (37 °C)
• J_O2,P(NS) = J_O2,P(GM)/0.73
• Fiber wet mass to dry mass ratio = 3.5

• Human vastus lateralis
• 6 females & males
• 50.2 years
• Sedentary, no regular leisure or training sporting activity
• H
• M
• BME
• BMI
• V_O2max/BM = 31.7 mL·min^-1·kg^-1 (= V_O2peak/BM/0.93)
• Permeabilized muscle fibres; 22 °C; GM_P; m_d; conversions: Gnaiger 2009 Int J Biochem Cell Biol
• J_O2,P(NS) = 55 µmol·s^-1·kg^-1 wet muscle mass (37 °C)

• J_O2,P(GM) = 40 µmol·s^-1·kg^-1 wet muscle mass (37 °C)
• J_O2,P(NS) = J_O2,P(GM)/0.73
• Fiber wet mass to dry mass ratio = 3.5

References: BME and VO2max

» VO2max

	Reference
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Labels: MiParea: Respiration, Exercise physiology;nutrition;life style 

Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 

Regulation: ADP  Coupling state: OXPHOS  Pathway: N 

VO2max, BMI, BME, MitoEAGLE BME