Difference between revisions of "O2k-Virtual Support self-study material"

Latest revision as of 12:50, 7 November 2023

O2k-Virtual Support self-study material

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

General information

To benefit optimally from the tutored training sessions of the O2k-Virtual Support, please make yourself familiar with Materials for self-study in advance.

O2k-Basic

Part 1.1: OroboPOS service and O2k instrumental

O2k-Manual: O2k-FluoRespirometer
O2k-Manual: POS Service
Step 1: O2k-Videosupport: OroboPOS from Perspex housing
Step 2: O2k-Videosupport: Cathode cleaning
Step 3: O2k-Videosupport: Anode cleaning
Step 4: O2k-Videosupport: Membrane mounting
Step 5: O2k-Videosupport: DL7 Start DatLab7
Step 6: O2k-Videosupport: DL7 Insert OroboPOS
Step 7: O2k-Videosupport: Insert O2k Chamber
Step 8: O2k-Videosupport: O2k Chamber volume calibration

Part 1.2: DatLab and O₂ calibration

Part 1.3: O₂ instrumental background

SOP: O₂ background

Part 2.1: Biological samples and experimental design

Pathway control and coupling control of mitochondrial respiration:

Sample types,mitochondrial preparations and sample addition:

SUIT protocols, SUITbrowser, DL-Protocols:

Part 2.2: Biological experiment and data analysis

O2k-Advanced

O2k-Applications: Simultaneous determination of O₂ and H₂O₂ fluxes

@@ Line 2: / Line 2: @@
 [[File:MITOEAGLE-representation.jpg|right|180px|link=http://www.mitoglobal.org/index.php/COST Action MitoEAGLE|COST Action MitoEAGLE]]
 __TOC__
+== General information ==
+:::: To benefit optimally from the tutored training sessions of the O2k-Virtual Support, please make yourself familiar with '''Materials for self-study''' in advance.
 == O2k-Basic ==
 === Part 1.1: OroboPOS service and O2k instrumental ===
 :::* [[O2k-FluoRespirometer|O2k-Manual: O2k-FluoRespirometer]]
 :::* [[MiPNet19.18B POS-service|O2k-Manual: POS Service]]
-:::* [https://www.youtube.com/watch?v=wMDcSypG3O4&feature=youtu.be O2k-Videosupport: OroboPOS]
+:::* Step 1: [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/wMDcSypG3O4]] [https://youtu.be/wMDcSypG3O4 O2k-Videosupport: OroboPOS from Perspex housing]
-:::* [https://www.youtube.com/watch?v=HC6MLXO08k8&feature=youtu.be O2k-Videosupport: Cathode cleaning]
+:::* Step 2: [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/HC6MLXO08k8]] [https://youtu.be/HC6MLXO08k8 O2k-Videosupport: Cathode cleaning]
-:::* [https://www.youtube.com/watch?v=sDoU69-SLq0&feature=youtu.be O2k-Videosupport: Anode cleaning]
+:::* Step 3: [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/sDoU69-SLq0]] [https://youtu.be/sDoU69-SLq0 O2k-Videosupport: Anode cleaning]
-:::* [https://www.youtube.com/watch?v=iGafaPa4OyA&feature=youtu.be O2k-Videosupport: Membrane mounting]
+:::* Step 4: [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/iGafaPa4OyA]] [https://youtu.be/iGafaPa4OyA O2k-Videosupport: Membrane mounting]
-:::* [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 O2k-Videosupport: Start DatLab 7]
+:::* Step 5: [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/0EfCReolzdk]] [https://youtu.be/0EfCReolzdk O2k-Videosupport: DL7 Start DatLab7]
-:::* [https://www.youtube.com/watch?v=pGzb0KfYwTQ&feature=youtu.be O2k-Videosupport: Insert OroboPOS]
+:::* Step 6: [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/pGzb0KfYwTQ]] [https://youtu.be/pGzb0KfYwTQ O2k-Videosupport: DL7 Insert OroboPOS]
-:::* [https://www.youtube.com/watch?v=1MSsI1ePMu8&feature=youtu.be O2k-Videosupport: Insert O2k Chamber]
+:::* Step 7: [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/1MSsI1ePMu8]] [https://youtu.be/1MSsI1ePMu8 O2k-Videosupport: Insert O2k Chamber]
-:::* [https://www.youtube.com/watch?v=DuaEJc7FBjk&feature=youtu.be O2k-Videosupport: O2k Chamber volume calibration]
+:::* Step 8: [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/zZI7O5zAruw]] [https://youtu.be/zZI7O5zAruw O2k-Videosupport: O2k Chamber volume calibration]
-=== Part 1.2: DatLab ===
+=== Part 1.2: DatLab and  O<sub>2</sub> calibration ===
 :::* [[MitoPedia: DatLab]]
+:::* [[MiPNet26.06 DatLab 7: Guide|O2k-Manual: DatLab 7 Guide]]
 :::* [[Run DL-Protocol/Set O2 limit|DL-Protocols]]
+:::* [[Gnaiger 2008 POS]]
+:::* [[MiPNet19.03 O2k-cleaning and ISS|SOP: O2k-cleaning and ISS]]
+:::* [[MiPNet06.03 POS-calibration-SOP|SOP: O<sub>2</sub>-calibration]]
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/onOIVpmOEoE]] [https://youtu.be/onOIVpmOEoE O2k-Videosupport: QC1: POS calibration]
-=== Part 1.3: O2calibration and instrumental background  ===
+=== Part 1.3: O<sub>2</sub> instrumental background  ===
-:::* [[Gnaiger 2008 POS]]
-:::* [[MiPNet19.03 O2k-cleaning and ISS|SOP: O2k-cleaning and ISS]]
+:::* [[MiPNet14.06 Instrumental O2 background|SOP: O<sub>2</sub> background]]
-:::* [[MiPNet06.03 POS-calibration-SOP|SOP: O2-calibration]]
-:::* O2k-Videosupport: QC1: POS calibration*
-:::* [[MiPNet14.06 Instrumental O2 background|SOP: O2 background]]
 === Part 2.1: Biological samples and experimental design ===
 '''Pathway control and coupling control of mitochondrial respiration:'''
-:::* [[Gnaiger 2020 MitoPathways|Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis]]
+:::* [[Gnaiger 2020 BEC MitoPathways|BEC 2020.2 Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis]]
 :::* [[BEC 2020.1 doi10.26124bec2020-0001.v1|BEC 2020.1 Mitochondrial Physiology]]
 '''Sample types,mitochondrial preparations and sample addition:'''
 :::* [[Sample]]
 :::* [[Mitochondrial preparations]]
-:::* [[MiPNet17.03 Shredder vs Fibres]]
+:::* [[MiPNet17.03 Shredder vs Fibres|Shredder ''vs'' Fibers]]
-:::* [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be O2k-Videosupport: Shredder tissue homogenate]
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/EjQGUr5M8v0]]  [https://youtu.be/EjQGUr5M8v0 O2k-Videosupport: Shredder tissue homogenate]
-:::* [https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be O2k-Videosupport: Fiber permeabilization]
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/iN38rOkiBUQ]]  [https://youtu.be/iN38rOkiBUQ O2k-Videosupport: Fiber permeabilization]
 :::* [[Cell count and normalization in HRR]]
-:::* O2k-Videosupport: Addition of biological sample to the respirometer chamber*
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/GX_SvnVGXgg]]  [https://youtu.be/GX_SvnVGXgg O2k-Videosupport: Addition of biological sample to the respirometer chamber]
 '''SUIT protocols, SUITbrowser, DL-Protocols:'''
 :::* [[MitoPedia: SUIT]]
 :::* [https://suitbrowser.oroboros.at/ Oroboros SUITbrowser]
-:::* [https://www.youtube.com/watch?v=8T33sp9KkJk O2k-Videosupport: How to find a DL-Protocol (DLP)]
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/8T33sp9KkJk]]  [https://youtu.be/8T33sp9KkJk O2k-Videosupport: How to find a DL-Protocol (DLP)]
-:::* [https://www.youtube.com/watch?v=Vd66dHx-MyI O2k-Videosupport: Export DL-Protocol User (*.DLPU)]
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/Vd66dHx-MyI]]  [https://youtu.be/Vd66dHx-MyI O2k-Videosupport: Export DL-Protocol User (*.DLPU)]
 === Part 2.2: Biological experiment and data analysis ===
-:::* [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4|Oxygen_flux_analysis]]
+:::* [[MiPNet19.14 SOP Hamilton_microsyringes|SOP: Hamilton microsyringes]]
-:::* [https://www.youtube.com/watch?v=HRAGvmaK01I O2k-Videosupport: O2flux analysis with DatLab 7.4]
+:::* [[MiPNet09.12 O2k-Titrations|O2k-Titrations]]
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/9j4ZtHmHiss]]  [https://youtu.be/9j4ZtHmHiss  O2k-Videosupport:  How to perform an experiment with a SUIT DL-Protocol (DLP)]
+:::* [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4|Oxygen flux analysis]]
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/HRAGvmaK01I]]  [https://youtu.be/HRAGvmaK01I O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4]
 == O2k-Advanced ==
-''' O2k-Applications: Simultaneous  determination  of  O2 and H2O2fluxes'''
+''' O2k-Applications: Simultaneous  determination  of  O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> fluxes'''
 :::* [[Amplex UltraRed]]
-:::* O2k-Videosupport: Smart Fluo-Sensor assembly*
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/nlVLXcbBRqs]]  [https://youtu.be/nlVLXcbBRqs O2k-Videosupport: Smart Fluo-Sensor assembly]
-:::* O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor*
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/eUukvgAi1yY]]  [https://youtu.be/eUukvgAi1yY O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor]
-:::* [[Amplex_UltraRed#Calibration_with_H2O2|Calibration with H2O2]]
+:::* [[Amplex_UltraRed#Calibration_with_H2O2|Calibration with H<sub>2</sub>O<sub>2</sub>]]
-:::* [[MiPNet24.10 H2O2 flux analysis|H2O2flux analysis]]
+:::* [[MiPNet24.10 H2O2 flux analysis|H<sub>2</sub>O<sub>2</sub> flux analysis]]
-:::* O2k-Videosupport: H2O2flux analysis with DatLab 7.4*
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/_Fv0VSA_c14]]  [https://youtu.be/_Fv0VSA_c14 O2k-Videosupport: H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4]
-== NextGen-O2k KOL Training ==
-:::* O2k-Videosupport: SUIT-030_PB_ce_D069 demo experiment *
-:::* O2k-Videosupport: SUIT-030_PB_ce_D070 demo experiment*
- * ''exclusive materials and links will be sent upon registration ''