Difference between revisions of "O2k-Virtual Support self-study material"

Revision as of 17:27, 19 October 2020

O2k-Virtual Support self-study material

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

General information

The materials for self-study, with the names and links to materials, are recommended to be studied before the respective training sessions of the Virtual O2k-Workshop.

O2k-Basic

Part 1.1: OroboPOS service and O2k instrumental

Part 1.2: DatLab

Part 1.3: O₂calibration and instrumental background

Part 2.1: Biological samples and experimental design

Pathway control and coupling control of mitochondrial respiration:

Sample types,mitochondrial preparations and sample addition:

Sample
Mitochondrial preparations
MiPNet17.03 Shredder vs Fibres
O2k-Videosupport: Shredder tissue homogenate
O2k-Videosupport: Fiber permeabilization
Cell count and normalization in HRR
O2k-Videosupport: Addition of biological sample to the respirometer chamber*

SUIT protocols, SUITbrowser, DL-Protocols:

Part 2.2: Biological experiment and data analysis

O2k-Advanced

O2k-Applications: Simultaneous determination of O₂ and H₂O₂ fluxes

Amplex UltraRed
O2k-Videosupport: Smart Fluo-Sensor assembly*
O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor*
Calibration with H₂O₂
H₂O₂ flux analysis
O2k-Videosupport: H₂O₂ flux analysis with DatLab 7.4*

NextGen-O2k KOL Training

O2k-Videosupport: SUIT-030_PB_ce_D069 demo experiment *
O2k-Videosupport: SUIT-030_PB_ce_D070 demo experiment*

* exclusive materials and links will be sent upon registration

@@ Line 61: / Line 61: @@
 :::* O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor*
 :::* [[Amplex_UltraRed#Calibration_with_H2O2|Calibration with H<sub>2</sub>O<sub>2</sub>]]
-:::* [[MiPNet24.10 H2O2 flux analysis|H´<sub>2</sub>O<sub>2</sub> flux analysis]]
+:::* [[MiPNet24.10 H2O2 flux analysis|H<sub>2</sub>O<sub>2</sub> flux analysis]]
 :::* O2k-Videosupport: H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4*