O2k-Virtual Support self-study material

                  

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O2k-Virtual Support self-study material

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MitoPedia O2k and high-resolution respirometry: O2k-Open Support 

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General information

To benefit optimally from the tutored training sessions of the Virtual O2k-Workshop, please make yourself familiar with Materials for self-study in advance. These materials are either Open Access (OA) or will be sent upon registration (R).

O2k-Basic

Part 1.1: OroboPOS service and O2k instrumental

• O2k-Manual: O2k-FluoRespirometer (OA)
• O2k-Manual: POS Service (OA)
• O2k-Videosupport: OroboPOS (OA)
• O2k-Videosupport: Cathode cleaning (OA)
• O2k-Videosupport: Anode cleaning (OA)
• O2k-Videosupport: Membrane mounting (OA)
• O2k-Videosupport: Start DatLab 7 (OA)
• O2k-Videosupport: Insert OroboPOS (OA)
• O2k-Videosupport: Insert O2k Chamber (OA)
• O2k-Videosupport: O2k Chamber volume calibration (OA)

Part 1.2: DatLab

• MitoPedia: DatLab (OA)
• DL-Protocols (OA)

Part 1.3: O₂ calibration and instrumental background

• Gnaiger 2008 POS (OA)
• SOP: O2k-cleaning and ISS (OA)
• SOP: O₂-calibration (OA)
• O2k-Videosupport: QC1: POS calibration (R)
• SOP: O₂ background (OA)

Part 2.1: Biological samples and experimental design

Pathway control and coupling control of mitochondrial respiration:

• BEC 2020.2 Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis (OA)
• BEC 2020.1 Mitochondrial Physiology (OA)

Sample types,mitochondrial preparations and sample addition:

• Sample (OA)
• Mitochondrial preparations (OA)
• MiPNet17.03 Shredder vs Fibres (OA)
• O2k-Videosupport: Shredder tissue homogenate (OA)
• O2k-Videosupport: Fiber permeabilization (OA)
• Cell count and normalization in HRR (OA)
• O2k-Videosupport: Addition of biological sample to the respirometer chamber (R)

SUIT protocols, SUITbrowser, DL-Protocols:

• MitoPedia: SUIT (OA)
• Oroboros SUITbrowser (OA)
• O2k-Videosupport: How to find a DL-Protocol (DLP) (OA)
• O2k-Videosupport: Export DL-Protocol User (*.DLPU) (OA)

Part 2.2: Biological experiment and data analysis

• Oxygen_flux_analysis (OA)
• O2k-Videosupport: O₂ flux analysis with DatLab 7.4 (OA)

O2k-Advanced

O2k-Applications: Simultaneous determination of O₂ and H₂O₂ fluxes

• Amplex UltraRed (OA)
• O2k-Videosupport: Smart Fluo-Sensor assembly (R)
• O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor (R)
• Calibration with H₂O₂ (OA)
• H₂O₂ flux analysis (OA)
• O2k-Videosupport: H₂O₂ flux analysis with DatLab 7.4 (R)

NextGen-O2k KOL Training

• PB-Module Manual (R)

• O2k-Videosupport: SUIT-030_PB_ce_D069 demo experiment (R)

• DL-Protocol: SUIT-030_PB_ce_D069 (R)
• O2k-Videosupport: SUIT-030_PB_ce_D070 demo experiment (R)

• DL-Protocol: SUIT-030_PB_ce_D070 (R)

• Q-Module

• Q-Module manual: MiPNet24.12 NextGen-O2k: Q-Module
• CV software manual: MiPNet24.16 DatLab8.0: CV-Module
• Videos: Q-Module