O2k-Virtual Support self-study material

                

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O2k-Virtual Support self-study material

                  

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O2k-Virtual Support self-study material

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MitoPedia O2k and high-resolution respirometry: O2k-Open Support 

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O2k-Basic

=== Part 1.1: OroboPOS service and O2k instrumental

• O2k-Manual: O2k-FluoRespirometer
• O2k-Manual: POS Service
• O2k-Videosupport: OroboPOS
• O2k-Videosupport: Cathode cleaning
• O2k-Videosupport: Anode cleaning
• O2k-Videosupport: Membrane mounting
• O2k-Videosupport: Start DatLab 7
• O2k-Videosupport: Insert OroboPOS
• O2k-Videosupport: Insert O2k Chamber
• O2k-Videosupport: O2k Chamber volume calibration

Part 1.2: DatLab

• MitoPedia: DatLab
• DL-Protocols

Part 1.3: O2calibration and instrumental background

• Gnaiger 2008 POS
• SOP: O2k-cleaning and ISS
• SOP: O2-calibration
• O2k-Videosupport: QC1: POS calibration*
• SOP: O2 background

Part 2.1: Biological samples and experimental design

Pathway control and coupling control of mitochondrial respiration:

• Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis
• BEC 2020.1 Mitochondrial Physiology

Sample types,mitochondrial preparations and sample addition:

• Sample
• Mitochondrial preparations
• MiPNet17.03 Shredder vs Fibres
• O2k-Videosupport: Shredder tissue homogenate
• O2k-Videosupport: Fiber permeabilization
• Cell count and normalization in HRR
• O2k-Videosupport: Addition of biological sample to the respirometer chamber*

SUIT protocols, SUITbrowser, DL-Protocols:

• MitoPedia: SUIT
• Oroboros SUITbrowser
• O2k-Videosupport: How to find a DL-Protocol (DLP)
• O2k-Videosupport: Export DL-Protocol User (*.DLPU)

Part 2.2: Biological experiment and data analysis

• Oxygen_flux_analysis
• O2k-Videosupport: O2flux analysis with DatLab 7.4

O2k-Advanced

O2k-Applications: Simultaneous determination of O2 and H2O2fluxes

• Amplex UltraRed
• O2k-Videosupport: Smart Fluo-Sensor assembly*
• O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor*
• Calibration with H2O2
• H2O2flux analysis
• O2k-Videosupport: H2O2flux analysis with DatLab 7.4*

NextGen-O2k KOL Training

• O2k-Videosupport: SUIT-030_PB_ce_D069 demo experiment *
• O2k-Videosupport: SUIT-030_PB_ce_D070 demo experiment*

* exclusive materials and links will be sent upon registration