Respiratory state: Difference between revisions
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== Coupling control states == | == Coupling control states == | ||
Coupling states and ''[[CCR]]'' of mitochondrial preparations: | ::: Coupling states and ''[[CCR]]'' of mitochondrial preparations: | ||
*[[OXPHOS]], [[LEAK]], [[ETS]] (''P, L, E'') - corrected for [[ROX]]. ''CCR'': ''[[L/E]]'', ''[[P/E]]'', ''[[L/P]]'' | ::::* [[OXPHOS]], [[LEAK]], [[ETS]] (''P, L, E'') - corrected for [[ROX]]. ''CCR'': ''[[L/E]]'', ''[[P/E]]'', ''[[L/P]]'' | ||
Coupling states of intact cells: | ::: Coupling states of intact cells: | ||
*[[ROUTINE]], [[LEAK]], [[ETS]] (''R, L, E'') - corrected for [[ROX]]. ''CCR'': ''[[L/E]]'', ''[[R/E]]'', ''[[L/R]]'' | ::::* [[ROUTINE]], [[LEAK]], [[ETS]] (''R, L, E'') - corrected for [[ROX]]. ''CCR'': ''[[L/E]]'', ''[[R/E]]'', ''[[L/R]]'' | ||
== Substrate control states == | == Substrate control states == | ||
Substrate control states, ''[[SCR]]'', are defined by substrate type (at saturating concentration) as '''pathway control states''': | :::: Substrate control states, ''[[SCR]]'', are defined by substrate type (at saturating concentration) as '''pathway control states''': | ||
*Intact cells: [[endogenous]], [[exogenous]] substrate control | ::::* Intact cells: [[endogenous]], [[exogenous]] substrate control | ||
*Mitochondrial preparations: specific substrate-inhibitor combinations for selectively stimulating electron entry though Complex I, CII, or other branches converging at the Q-junction, particularly with [[fatty acid oxidation]], alpha-[[glycerophosphate]], ([[Complex I-linked substrate state]], [[Complex II-linked substrate state]], etc.), or substrate combinations applied for reconstitution of [[TCA cycle]] function (e.g. [[Complex I&II-linked substrate state]], etc.). | ::::* Mitochondrial preparations: specific substrate-inhibitor combinations for selectively stimulating electron entry though Complex I, CII, or other branches converging at the Q-junction, particularly with [[fatty acid oxidation]], alpha-[[glycerophosphate]], ([[Complex I-linked substrate state]], [[Complex II-linked substrate state]], etc.), or substrate combinations applied for reconstitution of [[TCA cycle]] function (e.g. [[Complex I&II-linked substrate state]], etc.). | ||
Control by substrate concentration: '''Kinetic control states''': | ::: Control by substrate concentration: '''Kinetic control states''': | ||
*Kinetic substrate or adenylate control: Kinetic studies with variation of a specific substrate (reduced substrate supplying electrons to the ETS; ADP, Pi; O<sub>2</sub>; cytochrome ''c'') are analyzed by kinetic functions (e.g. hyperbolic), yielding apparent kinetic constants, such as ''J''<sub>max</sub>, ''K''<sub>m</sub>', ''c''<sub>50</sub>, or ''p''<sub>50</sub>. | ::::* Kinetic substrate or adenylate control: Kinetic studies with variation of a specific substrate (reduced substrate supplying electrons to the ETS; ADP, Pi; O<sub>2</sub>; cytochrome ''c'') are analyzed by kinetic functions (e.g. hyperbolic), yielding apparent kinetic constants, such as ''J''<sub>max</sub>, ''K''<sub>m</sub>', ''c''<sub>50</sub>, or ''p''<sub>50</sub>. | ||
*Kinetic inhibitor control: Kinetic studies with variation of a specific inhibitor yield apparent kinetic constants, such as the ''K''<sub>I</sub>'. | ::::* Kinetic inhibitor control: Kinetic studies with variation of a specific inhibitor yield apparent kinetic constants, such as the ''K''<sub>I</sub>'. | ||
== Classical respiratory states == | == Classical respiratory states == | ||
Chance and Williams (1955): | ::: Chance and Williams (1955): | ||
*[[State 1]], [[State 2]], [[State 3]], [[State 4]], [[State 5]]. ''CCR'': [[RCR]] | ::::* [[State 1]], [[State 2]], [[State 3]], [[State 4]], [[State 5]]. ''CCR'': [[RCR]] | ||
Derived respiratory states: | ::: Derived respiratory states: | ||
*[[State 3u]], [[State 4o]]. ''CCR'': [[UCR]] | ::::* [[State 3u]], [[State 4o]]. ''CCR'': [[UCR]] | ||
Thermodynamics of irreversible processes: | ::: Thermodynamics of irreversible processes: | ||
::::* [[Static head]], [[Level flow]] | |||
== MitoPedia: Respiratory states == | == MitoPedia: Respiratory states == | ||
* See the complete '''[[MitoPedia: Respiratory states]]''' | ::::* See the complete '''[[MitoPedia: Respiratory states]]''' | ||
::::* [[MitoPedia: SUIT]] |
Revision as of 09:17, 15 May 2016
Description
Respiratory states of mitochondrial preparations and intact cells are defined in the current literature in many ways and with a diversity of terms. Mitochondrial respiratory states must be defined in terms of both, the coupling control state and the substrate control state.
Reference: Gnaiger 2014 MitoPathways
MitoPedia concepts:
MiP concept,
Respiratory state,
SUIT concept
MitoPedia methods:
Respirometry,
Spectrophotometry
Coupling control states
- Coupling states and CCR of mitochondrial preparations:
- Coupling states of intact cells:
Substrate control states
- Substrate control states, SCR, are defined by substrate type (at saturating concentration) as pathway control states:
- Intact cells: endogenous, exogenous substrate control
- Mitochondrial preparations: specific substrate-inhibitor combinations for selectively stimulating electron entry though Complex I, CII, or other branches converging at the Q-junction, particularly with fatty acid oxidation, alpha-glycerophosphate, (Complex I-linked substrate state, Complex II-linked substrate state, etc.), or substrate combinations applied for reconstitution of TCA cycle function (e.g. Complex I&II-linked substrate state, etc.).
- Control by substrate concentration: Kinetic control states:
- Kinetic substrate or adenylate control: Kinetic studies with variation of a specific substrate (reduced substrate supplying electrons to the ETS; ADP, Pi; O2; cytochrome c) are analyzed by kinetic functions (e.g. hyperbolic), yielding apparent kinetic constants, such as Jmax, Km', c50, or p50.
- Kinetic inhibitor control: Kinetic studies with variation of a specific inhibitor yield apparent kinetic constants, such as the KI'.
Classical respiratory states
- Chance and Williams (1955):
- Derived respiratory states:
- Thermodynamics of irreversible processes:
MitoPedia: Respiratory states
- See the complete MitoPedia: Respiratory states
- MitoPedia: SUIT