S/NS pathway control ratio: Difference between revisions
No edit summary |
No edit summary |
||
Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|abbr=CII/CI&II | |abbr=CII/CI<small>&</small>II | ||
|description=The '''CII/CI&II [[substrate control ratio]]''' is obtained when [[rotenone]] (Rot) is added to [[CI&II]]-linked respiration in a defined coupling state. The reversed protocol, adding CI-linked substrates to a CII-linked [[background state]] does not provide a valid estimation of CII-linked respiration with succinate in the absence of Rot, since [[oxaloacetate]] accumulates as a potent inhibitor of [[succinate dehydrogenase]] (CII). | |description=The '''CII/CI<small>&</small>II [[substrate control ratio]]''' is obtained when [[rotenone]] (Rot) is added to [[CI&II]]-linked respiration in a defined coupling state. The reversed protocol, adding CI-linked substrates to a CII-linked [[background state]] does not provide a valid estimation of CII-linked respiration with succinate in the absence of Rot, since [[oxaloacetate]] accumulates as a potent inhibitor of [[succinate dehydrogenase]] (CII). | ||
|info=[[Gnaiger 2009 Int J Biochem Cell Biol]], [[Gnaiger | |info=[[Gnaiger 2009 Int J Biochem Cell Biol]], [[Gnaiger 2014 MitoPathways]] | ||
}} | }} | ||
{{MitoPedia methods | {{MitoPedia methods | ||
Line 12: | Line 12: | ||
= List of publications: CII and CI&II = | = List of publications: CII and CI<small>&</small>II = | ||
{{#ask:[[Category:Publications]] [[Substrate states::CII]] [[Substrate states::CI+II]] | {{#ask:[[Category:Publications]] [[Substrate states::CII]] [[Substrate states::CI+II]] | ||
|?Was published in year=Year | |?Was published in year=Year |
Revision as of 20:48, 31 August 2014
Description
The CII/CI&II substrate control ratio is obtained when rotenone (Rot) is added to CI&II-linked respiration in a defined coupling state. The reversed protocol, adding CI-linked substrates to a CII-linked background state does not provide a valid estimation of CII-linked respiration with succinate in the absence of Rot, since oxaloacetate accumulates as a potent inhibitor of succinate dehydrogenase (CII).
Abbreviation: CII/CI&II
Reference: Gnaiger 2009 Int J Biochem Cell Biol, Gnaiger 2014 MitoPathways
MitoPedia methods:
Respirometry
MitoPedia topics: "Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Respiratory control ratio"Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.