Difference between revisions of "SUIT-004"
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== Strengths and limitations== | == Strengths and limitations== | ||
:::*A succinate concentration of >10 mM may be required for saturating SE capacity. | |||
:::+ Linear coupling control (L-P-E) with N substrates (PM). | |||
:::+ Pathway control in ET state (N, NS and S pathways). | |||
:::+ This protocol can be extended with the Complex IV module. | |||
:::+ Short experimental time. | |||
- The substrate combination for maximum ET capacity (FNSGP) is not obtained in SUIT-004 in favor of reducing the experimental time. | |||
== Compare SUIT protocols == | == Compare SUIT protocols == |
Revision as of 11:17, 23 January 2019
Description
Abbreviation: NS(PM)
Reference: A: short RP1 »Versions
- SUIT-category: NS(PM)
- SUIT protocol pattern: orthogonal 1PM;2D;2c;3U;4S;5Rot;6Ama;8AsTm;9Az
Communicated by Doerrier C, Iglesias-Gonzalez J and Gnaiger E (last update 2019-01-21)
DLP applications
References
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1PM | PML(n) | N | CI | 1PM
|
2D | PMP | N | CI | 1PM;2D
|
2c | PMcP | N | CI | 1PM;2D;2c
|
3U | PME | N | CI | 1PM;2D;3U
|
4S | PMSE | NS | CI&II | 1PM;2D;3U;4S
|
5Rot | SE | S | CII | 1PM;2D;3U;4S;5Rot
|
6Ama | ROX | 1PM;2D;3U;4S;5Rot;6Ama
|
Step | Respiratory state | Pathway control | ET-Complex | Comment |
---|---|---|---|---|
## AsTm | AsTmE | CIV | CIV | |
## Azd | CHB |
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- A succinate concentration of >10 mM may be required for saturating SE capacity.
- + Linear coupling control (L-P-E) with N substrates (PM).
- + Pathway control in ET state (N, NS and S pathways).
- + This protocol can be extended with the Complex IV module.
- + Short experimental time.
- The substrate combination for maximum ET capacity (FNSGP) is not obtained in SUIT-004 in favor of reducing the experimental time.
Compare SUIT protocols
- SUIT-001 (RP1): SUIT-004 is a shortended version of RP1; without G, F and Gp.
MitoPedia concepts:
MiP concept,
SUIT protocol,
Recommended
MitoPedia methods:
Respirometry