SUIT-018 O2 mt D054

high-resolution terminology - matching measurements at high-resolution

SUIT-018 O2 mt D054

Description

Abbreviation: NS(GM)

Reference: B: SUIT-018

SUIT number: D054_1GMS;2D;3Ama

O2k-Application: O2

MitoPedia: SUIT - SUIT reference protocol

SUIT-category: NS(GM)

SUIT protocol pattern: lineal 1GMS;2D;3Ama

SUIT-018 O2 mt D054 is a short protocol to study th influence of the fluorescence dye on the O₂ flux. During the development of this protocol the [O₂] will be modified injecting nitrogen in the chamber.

Communicated by Komlodi T  (last update 2019-03-05)

Steps and respiratory states

Step	State	Pathway	Q-junction	Comment - Events (E) and Marks (M)
1GMS	GMSL	NS	CI&CII	1GMS Respiratory stimulation by simultaneous action of type N substrates & succinate, with convergent electron flow in the NS-pathway for reconstitution of TCA cycle function. Non-phosphorylating resting state (LEAK state); LEAK respiration L(n) in the absence of ADP, ATP, AMP (no adenylates).
2D	GMSP	NS	CI&CII	1GMS;2D Respiratory stimulation by simultaneous action of type N substrates & succinate, with convergent electron flow in the NS-pathway for reconstitution of TCA cycle function. OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
3Ama	ROX			1GMS;2D;3Ama Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).

Strengths and limitations

• This protocol can be used as a control for SUIT-018 AmR mt D031, SUIT-018 AmR mt D040 and SUIT-018 AmR mt D041. The oxygen concentration should be adjusted to the matching protocol with the Amplex UltraRed assay.

+ Reasonable duration of the experiment.

- CIV activity and cytochrome c test can be performed.

Compare SUIT protocols

MitoPedia concepts: SUIT protocol, SUIT B, Find 

MitoPedia methods: Respirometry