Difference between revisions of "SUIT-023"
From Bioblast
(10 intermediate revisions by 3 users not shown) | |||
Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|info='''A: Determination of | |abbr=AOX-ce SHAM+CN | ||
|description= [[File:ce1;ce2SHAM;ce3KCN.png|400px]]| | |||
|info='''A: Determination of respiration through the CIII-CIV pathway''' | |||
}} | }} | ||
::: '''[[SUIT protocol pattern]]:''' ce1;ce2SHAM;ce3KCN | |||
::: '''[[SUIT protocol pattern]]:''' | |||
The SUIT-023 | The SUIT-023 provides an estimation of mitochondrial respiration in living cells through the CIII-[[Complex_IV| cytochrome ''c'' oxidase]] pathway (CIII-CIV), which is inhibited by [[Antimycin_A| antimycin A]] and [[cyanide| cyanide]]. This protocol must be complemented with the study in parallel of mitochondrial respiration through the Q-[[alternative oxidase]] pathway (Q-AOX; see [[SUIT-022]]). | ||
__TOC__ | __TOC__ | ||
Communicated by [[Huete-Ortega M]] | Communicated by [[Huete-Ortega M]], [[Gnaiger E]] (last update 2021-03-23) | ||
== Specific SUIT protocols == | == Specific SUIT protocols == | ||
=== SUIT-023 O2 ce D053 === | === SUIT-023 O2 ce D053 === | ||
[[File:ce1;ce2SHAM;ce3KCN.png|400px]] | [[File:ce1;ce2SHAM;ce3KCN.png|400px]] [[File:ce1;ce2SHAM;ce3KCN.v2.png|400px]] | ||
[[File:D053_O2_traces.png|400px]] | [[File:D053_O2_traces.png|400px]] | ||
* [[SUIT-023 O2 ce D053]] for | * [[SUIT-023 O2 ce D053]] for living microalgal cells (ce) | ||
{{Template:SUIT-023}} | {{Template:SUIT-023 O2 ce D053}} | ||
== Strengths and limitations == | == Strengths and limitations == | ||
:::* SUIT-023 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-non-resistant mitochondrial respiration due to the activity | :::* SUIT-023 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-non-resistant mitochondrial respiration due to the activity [[Complex_IV| cytochrome ''c'' oxidase (CIV)]] in cells. To complement the study with the estimation of the mitochondrial respiration through the Q-[[alternative oxidase]] pathway, it must be carried out in parallel to [[SUIT-022]]. | ||
:::+ This protocol can be used with many | :::+ This protocol can be used with many types of cells, although optimal concentrations of inhibitors have to be tested. | ||
:::+ Short duration of experiment | :::+ Short duration of experiment | ||
:::- | :::- No estimation of coupling control | ||
:::- The use of [[cyanide| cyanide]] requires | :::- The use of [[cyanide| cyanide]] requires careful cleaning of the chambers after use and careful handling of the chemical to guarantee health safety. | ||
== Compare SUIT protocols == | == Compare SUIT protocols == | ||
::::*[[SUIT-022 O2 ce D051]] for [[microalgal]] | ::::*[[SUIT-022 O2 ce D051]] for [[microalgal]] living cells | ||
Line 48: | Line 49: | ||
{{MitoPedia concepts | {{MitoPedia concepts | ||
|mitopedia concept= | |mitopedia concept=SUIT protocol, Recommended | ||
}} | }} | ||
{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Respirometry | |mitopedia method=Respirometry | ||
}} | }} | ||
Latest revision as of 12:44, 23 March 2021
Description
Abbreviation: AOX-ce SHAM+CN
Reference: A: Determination of respiration through the CIII-CIV pathway
- SUIT protocol pattern: ce1;ce2SHAM;ce3KCN
The SUIT-023 provides an estimation of mitochondrial respiration in living cells through the CIII- cytochrome c oxidase pathway (CIII-CIV), which is inhibited by antimycin A and cyanide. This protocol must be complemented with the study in parallel of mitochondrial respiration through the Q-alternative oxidase pathway (Q-AOX; see SUIT-022).
Communicated by Huete-Ortega M, Gnaiger E (last update 2021-03-23)
Specific SUIT protocols
SUIT-023 O2 ce D053
- SUIT-023 O2 ce D053 for living microalgal cells (ce)
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
ce2SHAM | ROUTINE OR ROX | ce1;ce2SHAM
| ||
ce3KCN | ROX | ce1;ce2SHAM;ce3KCN
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- SUIT-023 provides an estimation of the cyanide-and antimycin A-non-resistant mitochondrial respiration due to the activity cytochrome c oxidase (CIV) in cells. To complement the study with the estimation of the mitochondrial respiration through the Q-alternative oxidase pathway, it must be carried out in parallel to SUIT-022.
- + This protocol can be used with many types of cells, although optimal concentrations of inhibitors have to be tested.
- + Short duration of experiment
- - No estimation of coupling control
- - The use of cyanide requires careful cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.
Compare SUIT protocols
- SUIT-022 O2 ce D051 for microalgal living cells
References
MitoPedia concepts: SUIT protocol, Recommended
MitoPedia methods:
Respirometry