Difference between revisions of "TPP+ inhibitory effect"
From Bioblast
Line 3: | Line 3: | ||
|info=[[MiPNet14.05]] | |info=[[MiPNet14.05]] | ||
}} | }} | ||
{{Template:Keywords Membrane potential}} | {{Template:Keywords Membrane potential}} | ||
Revision as of 21:53, 10 September 2018
Description
A major task in establishing a procedure for measurement of mitochondrial membrane potential using probe molecules is the evaluation of inhibitory concentrations of the probe molecule on the activity of respiration. The TPP+ inhibitory effect (this also applies to TPMP+ and other indicator molecules) is frequently ignored. Accurate knowledge of a threshold concentration is required to evaluate the necessary limit of detection of TPP+, and for restriction of experimental TPP+ concentrations below the inhibitory range.
Reference: MiPNet14.05
Template:Keywords Membrane potential
MitoPedia methods:
Respirometry
MitoPedia topics:
Inhibitor