Difference between revisions of "Warburg effect"
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:::* Ferreira LM (2010) Cancer metabolism: the Warburg effect today. Exp Mol Pathol 89:372-80. [PMID: 20804748] | :::* Ferreira LM (2010) Cancer metabolism: the Warburg effect today. Exp Mol Pathol 89:372-80. [PMID: 20804748] | ||
:::* Gnaiger E, Kemp RB (1990) Anaerobic metabolism in aerobic mammalian cells: information from the ratio of calorimetric heat flux and respirometric oxygen flux. Biochim Biophys Acta 1016:328-32. - [[Gnaiger 1990 Biochim Biophys Acta |Ā»Bioblast linkĀ«]] | |||
:::* Kim JW, Dang CV (2006) Cancerās molecular sweet tooth and the Warburg effect. Cancer Res 66:8927-30. Ā | :::* Kim JW, Dang CV (2006) Cancerās molecular sweet tooth and the Warburg effect. Cancer Res 66:8927-30. Ā |
Revision as of 11:20, 20 March 2020
Description
Requires definition
Reference: http://en.wikipedia.org/wiki/Warburg_effect
Oxygen and pH - Warburg versus Crabtree Effect
- Q: For quantification of aerobic glycolysis in intact cells, the measurement of proton production can be used as an indirect but continuous record of lactate production and corresponding acidification of the medium, while simultaneously monitoring oxygen concentration and oxygen consumption (MultiSensor-O2k). Is this related to the Warburg or Crabtree effect?
- A: Under various metabolic conditions, lactic acid production is the dominant mechanism causing acidification, hence the pH measurement is a good indirect indicator of aerobic glycolysis. An early paper summarizing the literature in this field states:
- "At high fructose concenĀtrations, respiration is inhibited while glycolytic end products accumulate, a phenomenon known as the Crabtree effect. It is commonly believed that this effect is restricĀted to microbial and tumour cells with uniquely high glycolytic capaciĀties (Sussman et al, 1980). HowĀever, inhibition of respiration and increase of lactate production are observed under aerobic condiĀtions in beating rat heart cell cultures (Frelin et al, 1974) and in isolated rat lung cells (Ayuso-Parrilla et al, 1978). Thus, the same general mechanisms responĀsible for the integraĀtion of respiration and glycolysis in tumour cells (Sussman et al, 1980) appear to be operating to some extent in several isolated mammalian cells." Gnaiger 1990 Biochim Biophys Acta
References
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- Warburg effect: "elevation of aerobic glycolysis seen in tumor cells"; "elevated lactate production under aerobic conditions".
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- Ferreira LM (2010) Cancer metabolism: the Warburg effect today. Exp Mol Pathol 89:372-80. [PMID: 20804748]
- Gnaiger E, Kemp RB (1990) Anaerobic metabolism in aerobic mammalian cells: information from the ratio of calorimetric heat flux and respirometric oxygen flux. Biochim Biophys Acta 1016:328-32. - Ā»Bioblast linkĀ«
- Kim JW, Dang CV (2006) Cancerās molecular sweet tooth and the Warburg effect. Cancer Res 66:8927-30.
- Liberti MV, Locasale JW (2016) The Warburg effect: how does it benefit cancer cells? Trends Biochem Sci 41:211ā8. [PubMed: 26778478]
- Potter M, Newport E, Morten KJ (2016) The Warburg effect: 80 years on. Biochem Soc Trans 44:1499ā505.
- Van der Heiden MG, Cantley LC, Thompson CB (2009) Understanding the Warburg effect: the metabolic requirements of cell proliferation. Science 324:1029-33.
- Warburg O (1956) On the origin of cancer cells. Science 123:309ā14.
- Warburg O (1956) On respiratory impairment in cancer cells. Science 124:269-70.
- Gentric G, Mieulet V, Mechta-Grigoriou F (2017) Heterogeneity in cancer metabolism: new concepts in an old field. Antioxid Redox Signal 26:462ā85. - http://online.liebertpub.com/doi/full/10.1089/ars.2016.6750
- Xing Yazhi, Zhao Shimin, Zhou Binhua P, Mi Jun (2015) Metabolic reprogramming of the tumour microenvironment. FEBS J 282:3892ā8. - http://onlinelibrary.wiley.com/doi/10.1111/febs.13402/full
MitoPedia methods: Respirometry